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	Provedor de dados BJM (3) Autor Aleixo,José Antonio Guimarães (1) Cardoso,Martha Oliveira (1) Carvalhal,José Beiro (1) Conceição,Rita de Cássia dos Santos da (1) Fernández,Bárbara (1) Gilardoni,Liliana Rosa (1) Goularte,Fabiana Lemos (1) Jar,Ana María (1) Mendez,Laura (1) Moreira,Ângela Nunes (1) Morsella,Claudia (1) Mundo,Silvia Leonor (1) Nascimento,Vladimir Pinheiro do (1) Paolicchi,Fernando Alberto (1) Ramos,Roberta Juliano (1) Ribeiro,Aldemir Reginato (1) Rocha,Silvio Luís da Silveira (1) Santos,Luciana Ruschel dos (1) Mais... Palavra-chave Immunomagnetic separation (3) Salmonella (2) Cow's milk (1) Mycobacterium avium subsp. paratuberculosis (1) PCR (1) Rapid methods (1) Raw broiler parts (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2016 (1) 2008 (1) 2002 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR BJM Gilardoni,Liliana Rosa; Fernández,Bárbara; Morsella,Claudia; Mendez,Laura; Jar,Ana María; Paolicchi,Fernando Alberto; Mundo,Silvia Leonor. Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected and uninfected herds in Argentina. IMS-IS1 PCR results were analyzed and compared with those obtained from milk and fecal culture and serum ELISA. The extent of agreement between both tests was determined by the Kappa test. IMS-IS1 PCR showed a detection limit of 101 CFU of Map/mL of milk, when 50:50 mix of monoclonal and polyclonal antibodies were used to coat magnetic beads. All of the 118 samples... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium avium subsp. paratuberculosis; Immunomagnetic separation; PCR; Cow's milk. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200506 Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts BJM Ribeiro,Aldemir Reginato; Nascimento,Vladimir Pinheiro do; Cardoso,Martha Oliveira; Santos,Luciana Ruschel dos; Rocha,Silvio Luís da Silveira. This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunomagnetic separation; Salmonella; Raw broiler parts. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400012 Detection of salmonella sp in chicken cuts using immunomagnetic separation BJM Conceição,Rita de Cássia dos Santos da; Moreira,Ângela Nunes; Ramos,Roberta Juliano; Goularte,Fabiana Lemos; Carvalhal,José Beiro; Aleixo,José Antonio Guimarães. The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional plating in selective agar (IMS-plating). First, protein A-coated microspheres were sensitized with polyclonal antibodies against lipopolysacharide and flagella from salmonellae and used to standardize a procedure for capturing Salmonella Enteritidis from pure cultures and detection in selective agar. Subsequently, samples of chicken meat experimentally contaminated with S.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Immunomagnetic separation; Rapid methods. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100034 Registros recuperados: 3 Primeira ... 1 ... Última

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