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Li,RS; Tao,YJ; Liu,FJ; Hu,X; Xu,QL; Li,KY. |
The grass pea (Lathyrus sativus L.) and flatpod peavine (Lathyrus cicera L.) are the most economically important and widely cultivated Lathyrus species. However, their utilization is limited due to the presence of their endogenous toxin β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP). Thus, a β-ODAP free variety should be developed through some plant breeding technique like either mutational breeding or genetic-manipulation. In this circumstance, the plant regeneration of Lathyrus species becomes a bottleneck. In the present study, an efficient system for in vitro regeneration of L. sativus with high β-ODAP levels, and L. cicera with low β-ODAP levels, was developed from different explants... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flatpod peavine; Grass pea; Green-nodular callus; In vitro regeneration. |
Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000100012 |
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Ouyang,Lejun; Huang,Zhenchi; Zhao,Longyan; Sha,Yuee; Zeng,Fuhua; Lu,Xiangyang. |
The aim of the present study was to establish an efficient regeneration system for the hybrid E. urophylla x E. grandis by means of organogenesis. Stem segments from seedlings were used as explants and cultured in a modified Murashige and Skoog medium (MS), supplemented with 13.2 µM N-phenyl-N'-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285 µM indole-3-acetic acid (IAA). PBU was a useful growth regulator. After cultivating for 5 d, 96% explants formed callus. After 30 d, the calli obtained were transferred to MS medium containing different combinations of 6-benzyladenine (BA) and naphthalene acetic acid (NAA). Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained browning. In addition, a large percentage... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro regeneration; Bud induction; Proliferation; Substituted urea. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300001 |
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