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| Rodrigues,Jaqueline Polizeli; Frei,Fernando; Navarro,Italmar Teodorico; Silva,Luciana Pereira; Marcelino,Monica Yonashiro; Andrade-Junior,Heitor Franco de; Faria,Carolina Arruda de; Santos,Marislene; Ribeiro-Paes,João Tadeu. |
| Background Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Toxoplasmosis; Enzyme-linked immunosorbent assay; Indirect fluorescent antibody test; Seroprevalence; Epidemiology. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100311 |
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| Antonello,A.M.; Cadore,G.C.; Pivoto,F.L.; Camillo,G.; Braunig,P.; Sangioni,L.A.; Pompermayer,E.; Gondim,L.F.P.; Vogel,F.S.F.. |
| The aim of this study was to examine the intra-uterine exposure to Sarcocystis spp. antigens, determining the number of foals with detectable concentrations of antibodies against these agents in the serum, before colostrum ingestion and collect data about exposure of horses to the parasite. Serum samples were collected from 195 thoroughbred mares and their newborns in two farms from southern Brazil. Parasite specific antibody responses to Sarcocystis antigens were detected using the indirect immunofluorescent antibody test (IFAT) and immunoblot analysis. In 84.1% (159/189) of the pregnant mares and in 7.4% (14/189) of foals we detected antibodies anti-Sarcocystis spp. by IFAT. All samples seropositive from foals were also positive in their respective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine protozoal mieloencephalitis; Transplacental infection; Seroprevalence; Indirect fluorescent antibody test; Immunoblot. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200271 |
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