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| Koltun,Alessandra; Erpen-Dalla Corte,Lígia; Mertz-Henning,Liliane Marcia; Gonçalves,Leandro SA. |
| ABSTRACT The burden of the current global challenge involving food security lies in the need to improve crop production. In this regard, biotechnology stands out as an essential tool to generate plants able to cope with pests, diseases, and harsh climatic conditions, and more efficient in the use of natural resources. An advanced approach to create genetic variability in a precise and targeted way, the genome-editing technique CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR associated proteins), has drawn the attention of breeders. The genome editing CRISPR/Cas system relies on a guiding RNA that directs a nuclease to generate a double-strand break (DSB) at a target DNA, activating the cell repair systems and eventually leading... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genome editing; Plant breeding; Biotechnology; Induced mutations; Non-transgenic. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000300290 |
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| López Colomba,E; Prina,A; Griffa,S; Ribotta,AN; Carloni,E; Tommasino,E; Luna,C; Biderbost,E; Grunberg,K. |
| Cenchrus ciliaris L., a forage grass of wide distribution in the north-west of Argentina, is a tetraploid (4x = 36) and obligate apomictic species. One way of obtaining novel germplasm is by induced mutations. In this work, physical and chemical mutations are combined with in vitro selection procedures seeking for new germplasm, with emphasis on salinity and drought tolerance. Mature seeds of Cenchrus ciliaris L. cv Biloela were subjected to treatments with X rays (400 Gy) and ethyl methanesulphonate (EMS) water solution (5.5 mM for 24 h). To perform in vitro selection, after 7 days of EMS or X rays treatments, germinated seeds were transferred to tubes containing Murashige and Skoog medium supplemented with NaCl or mannitol to simulate salinity and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cenchrus ciliaris; Genetic improvement; Induced mutations; In vitro selection; RAPDs analysis. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572011000100009 |
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