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	Provedor de dados Electron. J. Biotechnol. (1) Autor Arenas,Gloria (1) Díaz,Mauricio (1) Marchetti,Stefano (1) Marshall,Sergio H (1) Venturini,Elena (1) Palavra-chave Antimicrobial (1) Cecropin (1) Fusion (1) Intein (1) Peptide (1) Soluble (1) Mais... Tipo do documento Journal article (1) Ano 2012 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Intein-mediated expression of cecropin in Escherichia coli Electron. J. Biotechnol. Díaz,Mauricio; Venturini,Elena; Marchetti,Stefano; Arenas,Gloria; Marshall,Sergio H. Different strategies have been used to overcome the difficulties to produce antimicrobial peptides. Here we used Intein Mediated Purification with an Affinity Chitin-binding Tag (IMPACT-System, New England Biolabs) for the expression of the antimicrobial peptide cecropin to reduce its sensitivity to intracellular proteases and use its inducible self-cleaving capability to remove the carrier. Cecropin was cloned into suitable expression vector pTYB11, and expression induced by IPTG in Escherichia coli ER2566. The use of 22ºC induction allowed the expression of cecropin with its intein carrier in soluble form. Cell extracts were purified by chitin affinity chromatography and intein-mediated splicing of the target protein was achieved by thiol addition,... Tipo: Journal article Palavras-chave: Antimicrobial; Cecropin; Fusion; Intein; Peptide; Soluble. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200003 Registros recuperados: 1 Primeira ... 1 ... Última

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