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| Pereira,Daniela Isabel Brayer; Botton,Sônia de Avila; Azevedo,Maria Isabel de; Monteiro,Danieli Urach; Weiblen,Carla; Machado,Vanessa Schopf; Donatti,Michele Guimarães; Loeck,Alci Enimar. |
| Leaf-cutting ants of the genera Atta and Acromyrmex determine serious agricultural problems and live on symbiosis with Leucoagaricus gongylophorus. The aim of this study is to identify morphological and molecularly, as well as to verify the genotypic variability of the symbiotic fungus cultivated by A. heyeri and A. ambiguus from three different regions of the state of Rio Grande do Sul, Brazil. Fungus gardens were collected and fragments of mycelia were grown in selective medium. Total DNA was extracted and amplification of the ITS region was performed by PCR using universal primers. After DNA sequencing, the chromatograms were assembled and phylogenetic analyzes were performed by the Neighbor-Joining method. A total of six isolates of L. gongylophorus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Basidiomycete; Leucoagaricus gongylophorus; Internal transcribed spacer.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701256 |
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