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Transportation of Freeze-Dried Mouse Spermatozoa Under Different Preservation Conditions OAK
KAWASE, Yosuke; TACHIBE, Takanori; JISHAGE, Kou-ichi; SUZUKI, Hiroshi; 鈴木, 宏志.
Freeze-dried mouse spermatozoa can be used for normal embryonic development after injection into oocytes, thus indicating that freeze-drying is a useful method for the storage and transportation of genetic materials from animals. We recently reported that storage of freeze-dried mouse spermatozoa requires maintenance at temperatures lower than -80 C for long-term preservation and a pressure of 0.37 mbar at primary drying and that these conditions significantly improve the developmental rate to the blastocyst stage. In this study, we examined the influence of transportation and preservation conditions on freeze-dried spermatozoa. Freeze-dried spermatozoa stored for 2 or 2.5 years at 4 or -80 C were transported round trip overland between Shizuoka and...
Palavras-chave: Freeze-dried spermatozoa; Intra cytoplasmic sperm injection (ICSI); Mouse; Transportation.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3027
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