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| GOLUNSKI,SIMONE; SILVA,MARCELI F.; MARQUES,CAMILA T.; ROSSETO,VANUSA; KAIZER,ROSILENE R.; MOSSI,ALTEMIR J.; RIGO,DIANE; DALLAGO,ROGÉRIO M.; DI LUCCIO,MARCO; TREICHEL,HELEN. |
| ABSTRACT The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in the crude extract on the purification yield and purification factor were evaluated. Precipitation caused an activation of enzyme and allowed purification factors up to 2.4-fold for both alcohols. The purification factor was affected positively by the modification of the ionic strength of the medium to 0.5 mol.L-1 NaCl before precipitation with the alcohol (n-propyl or iso-propyl). A purification factor of 4.8-fold and an enzyme yield of 78.1 % could be achieved by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulinases; Ionic strength; Precipitation; Low cost.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100057 |
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| Fawzi,Eman Mohamed. |
| Thirty fungal species grown on Cichorium intybus L. root extract as a sole carbon source, were screened for the production of exo-inulinase activities. The thermophile Thielavia terrestris NRRL 8126 and mesophile Aspergillus foetidus NRRL 337 gave the highest production levels of inulinases I & II at 50 and 24 ºC respectively. Yeast extract and peptone were the best nitrogen sources for highest production of inulinases I & II at five and seven days of incubation respectively. The two inulinases I & II were purified to homogeneity by gel-filtration and ion-exchange chromatography with 66.0 and 42.0 fold of purification respectively. The optimum temperatures of purified inulinases I & II were 75 and 50 ºC respectively.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulinases; Production; Purification; Thermophile; Thermostability. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200028 |
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