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	Provedor de dados 58 (2) 52 (1) 59 (1) Autor Andrade,Agda (1) Bonfim,Tania M.B. (1) Brand,Debora (1) Dall’Agnol,Monique (1) Dall´Agnol,Monique (1) Gaensly,Fernanda (1) Kawaichi,Marisa Emiko (1) Martinez,Marina B. (1) Martinez,Marina Baquerizo (1) Newton,Salete (1) Picheth,Geraldo (1) Tokano,Dorismey Vieira (1) Venâncio,Emerson José (1) Vidotto,Marilda Carlos (1) Mais... Palavra-chave Iron uptake (4) Aerobactin receptor (1) Avian Escherichia coli (1) Biomass formation (1) EIEC (1) Enteroinvasive E. coli (1) Iron (1) Iron sources (1) IutA gene (1) Saccharomyces cerevisiae (1) Virulence factor (1) Yeast (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Info:eu-repo/semantics/other (1) Ano 2014 (1) 2008 (1) 2000 (1) 1999 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Cloning and characterization of the iron uptake gene iutA from avian Escherichia coli 52 Tokano,Dorismey Vieira; Kawaichi,Marisa Emiko; Venâncio,Emerson José; Vidotto,Marilda Carlos. The aim of this work was to isolate, clone and characterize the iron uptake gene iutA from avian pathogenic E. coli (APEC). The iutA gene was isolated from the strain APEC 9, serotype O2:H9, which was cloned in the expression vector pET101/D-TOPO. The gene of 2.2 Kb was sequenced (AY602767, which showed high similarity to the iutA gene from three plasmids, two from APEC, pAPEC-02-ColV (AY545598.4) and pTJ100 (AY553855.1), and one from a human invasive E. coli strain, the pColV K30. The recombinant protein IutA was over expressed in E. coli BL21(DE-3) and was solubilized with urea and purified by Ni-NTA column. This method produced a relatively high yield of r-IutA of approximately 74kDa, which was used to produce the antibody anti-IutA. This anti-IutA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian Escherichia coli; Iron uptake; Aerobactin receptor; Virulence factor; IutA gene. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300006 The uptake of different iron salts by the yeast Saccharomyces cerevisiae 58 Gaensly,Fernanda; Picheth,Geraldo; Brand,Debora; Bonfim,Tania M.B.. Yeasts can be enriched with microelements, including iron; however, special physicochemical conditions are required to formulate a culture media that promotes both yeast growth and iron uptake. Different iron sources do not affect biomass formation; however, considering efficacy, cost, stability, and compatibility with Saccharomyces cerevisiae metabolism, ferrous sulphate is recommended. Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Saccharomyces cerevisiae; Iron uptake; Iron sources; Biomass formation. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200016 The iron uptake mechanisms of enteroinvasive Escherichia coli 58 Andrade,Agda; Dall’Agnol,Monique; Newton,Salete; Martinez,Marina B.. Enteroinvasive Escherichia coli strains (EIEC) of different serotypes isolated from patients with acute diarrhea were examined for the ability to produce siderophores and iron-regulated outer membrane proteins (IROMP). For iron starvation cultures were grown at 37°C in LB supplied with 200 muM of <FONT FACE="Symbol">a</font>-alpha’dypirydil. All strains produced enterobactin and twelve (40%) produced aerobactin. The strains showed IROMP varying from 67-82 kDa. Proteins were either induced or stimulated by the iron starvation. Differences were observed in the electrophoretic profile among the serotypes, originating 5 electrophoretic profiles. All serotypes expressed proteins of 82 kDa (FepA) and 76 kDa (IutA) (except serotype O28ac:H- that did... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteroinvasive E. coli; Iron uptake. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300009 Uptake of iron from different compounds by enteroinvasive Escherichia coli 59 Dall´Agnol,Monique; Martinez,Marina Baquerizo. This work aimed the study of acquisition of iron from different compounds by EIEC strains. Seven serotypes were studied (O28ac.H-, O29:H-, O136:H-, O144:H-, O152:H-, O164:H- and O167:H7-), in a total of 31 strains. All serotypes were able to use distinct iron sources: hemin, hematin, hemoglobin, ovotransferin, lactoferrin, transferrin and ferritin. Tipo: Info:eu-repo/semantics/other Palavras-chave: EIEC; Iron; Iron uptake. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200012 Registros recuperados: 4 Primeira ... 1 ... Última

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