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Biotin-avidin sandwich elisa with specific human isotypes IgG1 and IgG4 for Culicidae mosquito blood meal identification from an epizootic yellow fever area in Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Marassá,AM; Paula,MB; Gomes,AC; Consales,CA.
With a view toward investigating the feeding behavior of Culicidae mosquitoes from an area of epizootic yellow fever transmission in the municipalities of Garruchos and Santo Antônio das Missões, Rio Grande do Sul State, Brazil, specimens were collected by aspiration from September 2005 to April 2007. The engorged females were submitted to blood meal identification by enzyme-linked immunosorbent assay (ELISA). A total of 142 blood-engorged samples were examined for human or monkey blood through species-specific IgG. Additional tests for specificity utilizing isotypes IgG1 and IgG4 of human monoclonal antibodies showed that only anti-human IgG1 was effective in recognizing blood meals of human origin. The results indicated a significant difference (p =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Culicidae; ELISA; Feeding habits; Isotypes; Yellow fever.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400008
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Lack of correlation between rabies virus replication in the brain and antibody isotype profile in genetically modified mice J. Venom. Anim. Toxins incl. Trop. Dis.
Consales,C. A.; Pereira,C. A.; Passos,E. C.; Carrieri,M. L.; Galina,N. M. F.; Sant'Anna,O. A..
The relationship among the phenotypes resistance to infection, virus replication in the brain and isotype production was investigated in genetically modified High (H) or Low (L) antibody responder mouse lines. Although they express the same innate susceptibility to rabies infection, these lines differ as to different viral replication rates in the central nervous system and L mice showed a higher permissible state. After intramuscular infection with the Pasteur rabies strain (PV), the H-L interline differences on the earlier stage of virus replication were 1000 and 80 folds on days 5 and 6, respectively. The isotype profile in sera of the experimentally infected mice reflected an interline difference of 25 folds for IgG2a throughout the infection period,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies; Infection; Isotypes; Nervous system.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300007
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Aging and immunoglobulin isotype patterns in oral tolerance BJMBR
Faria,A.M.C.; Ficker,S.M.; Speziali,E.; Menezes,J.S.; Stransky,B.; Verdolin,B.A.; Lahmann,W.M.; Rodrigues,V.S.; Vaz,N.M..
In the present review we address oral tolerance as an important biological phenomenon and discuss how it is affected by aging. Other factors such as frequency of feeding and previous digestion of the antigen also seem to influence the establishment of oral tolerance. We also analyze immunoglobulin isotypes of specific antibodies formed by tolerant and immunized animals of different ages submitted to different conditions of oral antigen administration. Isotypic patterns were studied as a parameter for assessing the pathways of B and T cell interactions leading to antibody production
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oral tolerance; Aging; Isotypes; Gut; Mucosa.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100005
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