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Determination of drying characteristics and kinetics of bitter kola (garcinia kola) using page’s model 111
Chinaka, Ehiem James.
Single-layer drying characteristics and kinetics of garcinia kola was studied with slice thicknesses of 1mm, 3mm, 5mm and 7mm at temperatures 45oC, 55oC, 65oC and 75oC using natural convention laboratory oven . The results obtained revealed that garcinia kola slices dried in the falling rate period. Moisture ratio, characteristics drying rate constant, drying time, effective diffusivity and activation energy were significantly (p
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural & Bioresources Engineering/Postharvest Processing and Storage Engineering Garcinia kola; Kinetics; Diffusion; Activation energy; Page’s model.
Ano: 2014 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2987
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Adsorption of p-cresol on Granular Activated Carbon 111
Lalitendu Das; North Carolina State University Biological and Agricultural Engineering; Praveen Kolar; North Carolina State University; Jason Osborne; North Carolina State University Department of Statistics; John Classen; North Carolina State University Biological and Agricultural Engineering.
Swine farming emit several odorous volatile organic compounds, one of which is p-cresol. Considering the layout of a swine barn, adsorption is one of the most suitable technologies for mitigating organic pollutants. In this study, commercial granular activated carbon (GAC) was tested as an adsorbent for removing p-cresol from aqueous solution. The objectives were to: 1) determine the combined effect of temperature, pH, and adsorbent dose on adsorption, (2) investigate the effect of volatile fatty acids and aldehydes on adsorption of p-cresol, (3) determine adsorption kinetics and isotherms, (4) study the effect of solvent on adsorption, (5) propose a possible mechanism of adsorption, and (6) discuss practical implications and design calculations for...
Palavras-chave: Agricultural Engineering Adsorption; P-cresol; Activated carbon; Kinetics; Isotherms; RSM.
Ano: 2012 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2171
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Requirements of mixed tangerine (Citrus tangerine) and pineapple (Ananas comosus) powdered peel wastes fermentation for citric acid production 111
Kumar, Prasanna GV; Paul, Sanjib Kumar; Khobragade, Chetan B; Bania, Bhargav Kumar; Sengupta, Debopriyo Kumar.
In this study, mixed substrate submerged fermentation of fruit peel wastes for the production of citric acid is reported. Powders of the peel wastes of tangerine and pineapple were used as substrates. Methanol (1-3% w/w) and sucrose (5-15% w/w) were supplemented in the fermentation media (pH on the range of 2.0-6.0) with moisture content of 90% (wet basis). Effect of substrate mix, pH of the media, methanol and sucrose concentrations on the yield of citric acid was studied, and their optimal levels for the maximum yield of citric acid were identified based on Taguchi method.  Methanol as stimulant in the medium and ratio of substrates in the mix had the highest influence on the yield of citric acid.  Fermentation media with tangerine and pineapple peel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromatography; Kinetics; Mixed substrate fermentation; Optimization; Synergism; Taguchi method.
Ano: 2020 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/5487
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Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes 45
Adona,P.R.; Leal,C.L.V..
The effect of concentration and exposure period of bovine oocytes to butyrolactone I (BLI) on meiotic block and in vitro maturation (IVM) kinetics was studied. In experiment 1, all oocytes were at germinal vesicle stage (GV), after 6h in culture with 0, 50 and 100µM BLI. After 12h, all oocytes cultured with 50 and 100µM BLI remained in GV. After 24h, less oocytes were in GV with 50µM (82%) than with 100µM BLI (99%, P<0.05). In experiment 2, after 6h IVM, 93% of control oocytes (IVM only) were in GV, while treated oocytes (100µM BLI for 6, 12 or 24h prior to IVM) showed less oocytes in GV with increased exposure period to BLI prior to IVM (83 and 73%, for 6h and 12h, P<0.05). For a 24h inhibition, GV rates were similar to 12h (70%, P>0.05). After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Meiosis; Oocyte; Butyrolactone I; Kinetics; Nuclear maturation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300011
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In situ dry matter degradability of three tropical forages of green chopped and ensiled forms 45
Cavalcante,D.R.; Perin,F.B.; Benedetti,E..
Dry matter (DM) ruminal degradability of corn (Zea mays L.), millet (Pennisetum glaucum (L. R. Br.) and sorghum (Sorghum bicolor L. Moench.) was evaluated using in situ technique with samples in green chopped and ensiled forms. Two crossbred fistulated (live weight of 480kg) dry cows participated. Samples of six grams in each forage were incubated in the rumen for 6, 24 and 96 hours. We estimated time zero (t0) washing the bags in water and it was used to calculate solubility. The experimental design followed a randomized block design with a split plot. We compared the average of DM through the SNK test at 5% probability. The results of disappearance (%) of dry matter forages of green chopped and ensiled forms in 0, 6, 24 and 96 hours were respectively:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromatology plant; Corn; Kinetics; Millet; Sorghum.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000100023
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Antioxidant activity of the extracts from Pycnoporus sanguineus mycelium 52
Borderes,Josiane; Costa,Alessandra; Guedes,Alessandro; Tavares,Lorena Benathar Ballod.
The aim of this work was to study the production of compounds with antioxidant activity by Pycnoporus sanguineus when cultivated in submerged fermentation using a potato dextrose broth plus peptone medium. The study evaluated the biomass production, glucose consumption, variation of medium pH and antioxidant activity. The antioxidant potential was tested through the DPPH method and β-carotene / linoleic acid system with extracts obtained from the mycelium at different times of cultivation (5, 10, 15, 20, 25 and 30 days). Maximum kinetic values of specific growth rate (0.289 day-1), biomass productivity (0.698 g.L-1.day-1) and the yield of glucose conversion into biomass (26.24 g.g-1) were obtained during the exponential growth phase. The highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Basidiomycetes; Submerged culture; Kinetics; Antioxidant compounds.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600012
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Production of spores of Trichoderma harzianum on sugar cane molasses and bagasse pith in solid state fermentation for biocontrol 52
Rodríguez-León,Jose A.; Domenech,F.; León,M.; Méndez,T.; Rodríguez,D. E.; Pandey,Ashok.
Solid state fermentation was carried out for the production of spores from Trichoderma harzianum No 53 using sugar cane bagasse pith as solid matrix and sugar cane molasses as carbon and energy source. Different nitrogen sources such as urea, (NH4)2SO4 , NH4H2PO4 and (NH4)2HPO4 were added in the media to test their effect on spores production. Among these, urea was found most useful that resulted high no of spores (1x10(9)/gDM). The influence of temperature and initial moisture of the substrate was studied through a 2² experimental plan design. No statistical differences were found within the range of 30-35ºC and 60-70% for temperature and moisture respectively. The biotechnological parameters of the process were derived from the Oxygen Uptake Rate (OUR)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Solid state fermentation; Sugar cane pith and molasses; Biocontrol; Kinetics.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100010
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Influence of passion fruit juice on colour stability and sensory acceptability of non-sugar Yacon-based pastes 52
Granato,Daniel; Castro,Inar Alves de; Piekarski,Flávia Vilas Boas Wiecheteck; Benincá,Cristina; Masson,Maria Lucia.
This work was aimed at studying the influence of PFJ and/or sodium disulphite on Yacon pulp colour, and evaluating the sensory attributes of Yacon non-sugar pastes. A colour kinetic evaluation of browning in 11 treatments containing Yacon tubers, yellow passion fruit juice (PFJ) and/or sodium disulphite, over the course of 240 minutes was studied to inactivate the PPOs activity. The evaluation of the colour alterations of Yacon tuber by the addition of variable amounts of PFJ and sodium disulphite enabled identification of efficient treatments for the inhibition of browning: T7 (300 g kg -1 PFJ), T8 (400 g kg -1 PFJ), and T10 (150 g kg -1 PFJ + 0.25 g kg -1 sodium disulphite). These treatments presented high colour intensity (C*) and an insignificant (P...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Smallanthus sonchifolius; Diabetes; Passion fruit; Sensory analysis; Kinetics; Polyphenol oxidases.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100020
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Ionically Bound Peroxidase from Peach Fruit 52
Neves,Valdir Augusto.
Soluble, ionically bound peroxidase (POD) and polyphenoloxidase (PPO) were extracted from the pulp of peach fruit during ripening at 20°C. Ionically bound form was purified 6.1-fold by DEAE-cellulose and Sephadex G-100 chromatography. The purified enzyme showed only one peak of activity on Sephadex G-100 and PAGE revealed that the enzyme was purified by the procedures adopted. The purified enzyme showed a molecular weight of 29000 Da, maximum activity at pH 5.0 and at 40ºC. The calculated apparent activation energy (Ea) for the reaction was10.04 kcal/mol. The enzyme was heat-labile in the temperature range of 60 to 75ºC with a fast inactivation at 75ºC. Measurement of residual activity showed a stabilizing effect of sucrose at various temperature/sugar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peach peroxidases; Ripening; Purification; Kinetics; Heat stability.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100002
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Respirometry kinetics of phenol oxidation by Comamonas testosteroni Pb50 under various conditions of nutritional stress 52
Paca,Jan; Kosteckova,Alena; Pacova,Leona; Prell,Ales; Halecky,Martin; Paca Jr.,Jan; Stiborova,Marie; Kozliak,Evguenii; Soccol,Carlos Ricardo.
Kinetics of phenol biodegradation using suspended biomass of Comamonas testosteroni Pb50 (monoculture) was measured under conditions of nutrient abundance, limitation, and prolonged cell starvation in a fed-batch reactor, with phenol being the sole carbon and energy source. The pre-washed cells were applied for measurement of the phenol and oxygen uptake rates at varied starting phenol concentrations with the kinetic parameters calculated using the Haldane model. The results revealed that nutrient limitation significantly suppressed the maximum value of exogenous respiration rate while the endogenous respiration rate, affinity and tolerance to phenol increased. By contrast, cell starvation resulted in a drop of both the exogenous and endogenous respiration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Respirometry; Kinetics; Phenol; Nutrition stress.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600030
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Partial Purification and Characterization of β-glucosidase fromMonascus sanguineus 52
Dikshit,Rashmi; Tallapragada,Padmavathi.
The aim of the present work was to study the production and characterization of β-glucosidase from Monascus sanguineus. Agro-waste residues were screened to obtain the maximum yield of enzyme. Jack fruit seed was the best substrate for enzyme production. Studies on the optimization of pH and temperature showed acidic pH favorable for enzymatic activity, whereas the optimum temperature was 60°C. Enzyme kinetics studies with different concentration of pNPG showed the calculated value of Km approximately 0.89 mM with the non-linear regression and 0.98 mM with the linear regression techniques. The enzyme was predominantly inhibited by KCl (69.8%) and moderately inhibited by CaCl2(14.8%). Studies on the sensitivity for glucose showed that after 100 mM...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zymography; Agro-waste; Kinetics; Monascus sanguineus; PNPG.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200185
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Kinetics Study of Extracellular Detergent Stable Alkaline Protease from Rhizopus oryzae 52
Mushtaq,Zareena; Irfan,Muhammad; Nadeem,Muhammad; Naz,Mammona; Syed,Quratulain.
In this study, extracellular alkaline protease was produced from Rhizopus oryzae in submerged fermentation using dairy waste (whey) as a substrate. Fermentation kinetics was studied and various parameters were optimized. The strain produced maximum protease at initial medium pH of 6.0 medium depth of 26 mm, inoculum size of 2% at incubation temperature of 35ºC for 168 h of fermentation. Alkaline protease was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of alkaline protease was 69 kDa determined by 10% SDS-PAGE. The optimum pH and temperature of alkaline protease was 9.0 and 40ºC, respectively. Metal profile of the enzyme showed that the enzyme was non-metallic in nature. The Km ,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kinetics; Purification; Characterization; Protease; Detergent stability; Rhizopus oryzae.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200175
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A broad pH range and processive chitinase from a metagenome library 56
Thimoteo,S.S.; Glogauer,A.; Faoro,H.; de Souza,E.M.; Huergo,L.F.; Moerschbacher,B.M.; Pedrosa,F.O..
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein. The MetaChi18A enzyme is a 92-kDa protein with a conserved active site domain of glycosyl hydrolases family 18. It hydrolyses colloidal chitin with an optimum pH of 5 and temperature of 50°C. Moreover, the enzyme...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Metagenomic; Aeromonas; Kinetics.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100602
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Kinetics of acid-base parameters in conventional hemodialysis 56
Lugon,J.R.; Pereira,G.R.M.; Strogoff-de-Matos,J.P.; Peixoto,A.J..
Details about the acid-base changes in hemodialysis are scarce in the literature but are potentially relevant to adequate management of patients. We addressed the acid-base kinetics during hemodialysis and throughout the interdialytic period in a cross-sectional study of adults undergoing conventional hemodialysis. Samples for blood gas analysis were obtained from the arterial limb of the arteriovenous fistula before the first session of the week (HD1), immediately at the end of HD1, and on sequential collections at 15, 30, 45, 60, and 120 min post-HD1. Additional blood samples were collected after ∼20 h following the end of the first dialysis and immediately prior to the initiation of the second dialysis of the week. Thirty adult patients were analyzed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemodialysis; Renal replacement therapy; Kinetics; Adequacy of dialysis; Acid-base.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100611
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Kinetics of high-Level of ß-glucosidase production by a 2-deoxyglucose-resistant mutant of Humicola lanuginosa in submerged fermentation 58
Bokhari,Syed Ali Imran; Latif,Farooq; Rajoka,Muhammad Ibrahim.
A 2-deoxyglucose-resistant mutant (M7) of Humicola lanuginosa was obtained by exposing conidia to γ-rays and permitting expression in broth containing 0.6% 2-deoxyglucose (DG) and cellobiose (1%) before plating on DG esculin-ferric ammonium citrate agar medium from which colonies showing faster and bigger blackening zones were selected. Kinetic parameters for enhanced ß-glucosidase (BGL) synthesis by M7 were achieved when corncobs acted as the carbon source. The combination between corncobs and corn steep liquor was the best to support higher values of all product formation kinetic parameters. Effect of temperature on the kinetic and thermodynamic attributes of BGL production equilibrium in the wild organismand M7was studied using batch process at eight...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SS-Glucosidase; Enthalpy; Entropy; Hemicellulose; Kinetics; Regulation; Submerged fermentation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400024
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Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor 58
Mukhtar,Hamid; Ikram-ul-Haq,.
We describe the simultaneous production of Bacillus subtilis based proteases and alpha amylase using a computer controlled laboratory scale 7.5 L batch bioreactor. The present strain is the first to be reported that concomitantly produces these two industrially important enzymes. The growth and sporulation of Bacillus subtilis was monitored and maximum production of alkaline protease and alpha amylase was found to coincide with maximum sporulation. Two types of proteases were detected in the fermentation broth; a neutral and an alkaline protease most active in a pH range of 7.0-8.0 and 8.0-10, respectively. Maximum production of proteases was observed at an incubation temperature of 37ºC while that of alpha amylase was observed at 40ºC. The optimum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Simultaneous; Sporulation; Kinetics; Fermentation; Agitation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300033
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Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001 58
Gumel,A.M.; Annuar,M.S.M.; Heidelberg,T..
Growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. Models with substrate inhibition terms described well the kinetics of its growth. Selected fatty acids (C8:0 to C18:1) and ammonium were used as carbon and nitrogen sources during growth and PHA biosynthesis, resulting in PHA accumulation of about 50 to 69% (w/w) and PHA yields ranging from 10.12 g L-1 to 15.45 g L-1, respectively. The monomer composition of the PHA ranges from C4 to C14, and was strongly influenced by the type of carbon substrate fed. Interestingly, an odd carbon chain length (C7) monomer was also detected when C18:1 was fed. Polymer showed melting temperature (Tm) of 42.0...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biopolymers; Kinetics; Polyesters; Polyhydroxyalkanoates; Pseudomonas.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200009
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Production of extracellular lipases by Rhizopus oligosporus in a stirred fermentor 58
Iftikhar,Tehreema; Niaz,Mubashir; Zia,Muhammad Anjum; Haq,Ikram ul.
The present investigation deals with the kinetics of submerged extracellular lipases fermentation by both wild and mutant strains of Rhizopus oligosporus var. microsporus in a laboratory scale stirred fermentor. Other parameters studied were inoculum size, pH, agitation and rate of aeration. It was found that the growth and lipases production was increased gradually and reached its maximum 9.07± 0.42ª U mL-1 (W) and 42.49 ± 3.91ª U mL-1 (M) after 30h of fermentation for both wild and mutant strain. There is overall increase of 109% (W) and 124% (M) in the production of extracellular lipases as compared to shake flask. Another significant finding of the present study is that the fermentation period is reduced to 30 h in case of wild and 23 h in case of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular lipases; Rhizopus oligosporus; Fermentor; Kinetics.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400034
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Preformulation of a liquid dosage formulation of captopril for pediatric use: drug-excipient compatibility and stability studies 60
Goes,Janaina da Silva; Freire,Fátima Duarte; Moura,Túlio Flávio Accioly de Lima e; Aragão,Cícero Flávio Soares; Raffin,Fernanda Nervo.
Currently, medications used in children are typically modified from pharmaceutical dosage forms designed for adults. Captopril is widely adapted to liquid formulations for use in hospitals. Its stability in the aqueous medium is reduced since it undergoes oxidation producing captopril disulfide (its main metabolite). The aim of this formulation study was to suggest favorable conditions for the development of a stable captopril formulation. The compatibility between the drug and excipients was evaluated by differential scanning calorimetry analysis (DSC). For studies in solution, different formulations were prepared according to a factorial design varying EDTA concentration, water purity and pH. The resultant formulations were stored at 60°C and analyzed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pediatric Formulation; Captopril/ pharmacokinetics; Thermal Analysis; Degradation Kinetics; Drug Stability; Drug Compounding/ utilization; Drug Utilization; Excipients/ pharmacokinetics; Dosage Forms; Drug Dosage Calculations; Kinetics; Enzyme Stability/drug effects.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100603
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Blackberry pomace microspheres: An approach on anthocyanin degradation 64
Santos,Suelen Siqueira dos; Paraíso,Carolina Moser; Madrona,Grasiele Scaramal.
ABSTRACT Blackberry pomace is a rich source of antioxidant compounds, including anthocyanins, but these compounds degrade easily in the presence of high temperatures. Therefore, the present study aimed to evaluate the effect of temperature on anthocyanin degradation in spray-dried blackberry pomace extract. Maltodextrin was used as a drying aid agent in a spray drying process to produce microspheres. The experiment was set up at Maringá-PR, Brazil (23º 25’ 31” S, 51º 56’ 19” W, 596 m altitude). The thermal stability of anthocyanins was evaluated in the presence and absence of copigments at different temperatures ranging from 70 °C to 100 °C using degradation kinetics. The role of maltodextrin in protecting anthocyanins during the spray drying process was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rubus fruticosus; Antioxidant analyses; Spray dryer; Kinetics.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542020000100406
Registros recuperados: 47
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