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	Provedor de dados Electron. J. Biotechnol. (2) BJM (1) Autor Dagbagli,Seval (1) Goksungur,Yekta (1) Klanrit,Preekamol (1) Moeini,Hassan (1) Nahvi,Iraj (1) Premjet,Siripong (1) Rungrattanakasin,Budsayachat (1) Tavassoli,Manoochehr (1) Thanonkeo,Pornthap (1) Thanonkeo,Sudarat (1) Mais... Palavra-chave Kluyveromyces lactis (3) Aspergillus fumigatus (1) B-galactosidase (1) BOD (1) Beta-galactosidase (1) Candida versatilis (1) Cellulosic material (1) Gene cloning (1) Kluyveromyces marxianus (1) Mixed culture (1) Response surface methodology (1) SCP (1) Shake flask culture (1) Synthetic medium (1) Thermotolerant fungus (1) Whey (1) Mais... Tipo do documento Journal article (2) Info:eu-repo/semantics/article (1) Ano 2018 (1) 2008 (1) 2004 (1) País Chile (2) Brazil (1) Idioma Inglês (2) Espanhol (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis BJM Rungrattanakasin,Budsayachat; Premjet,Siripong; Thanonkeo,Sudarat; Klanrit,Preekamol; Thanonkeo,Pornthap. Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and encoded a protein of 460 amino acid residues. The predicted molecular weight and the isoelectric point of the A. fumigatus Cel7 gene product were 48.19 kDa and 5.03, respectively. A catalytic domain in the N-terminal region and a fungal type cellulose-binding domain/module in the C-terminal region were detected in the predicted polypeptide sequences. Furthermore, a signal peptide with 20 amino acid residues at the N-terminus was also detected in the deduced... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus fumigatus; Cellulosic material; Gene cloning; Kluyveromyces lactis; Thermotolerant fungus. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300647 Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology Electron. J. Biotechnol. Dagbagli,Seval; Goksungur,Yekta. This paper investigates the production and optimization of b-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four fermentation parameters (agitation speed, pH, initial substrate concentration and incubation time) on b-galactosidase enzyme production. Results of the statistical analysis showed that the fit of the model was good in all cases. Maximum specific enzyme activity of 4218.4 U g-1 was obtained at the optimum levels of process variables (pH 7.35, agitation speed 179.2 rpm,... Tipo: Journal article Palavras-chave: B-galactosidase; Kluyveromyces lactis; Response surface methodology; Shake flask culture; Synthetic medium. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400011 Improvement of SCP production and BOD removal of whey with mixed yeast culture Electron. J. Biotechnol. Moeini,Hassan; Nahvi,Iraj; Tavassoli,Manoochehr. This research emphasizes on single cell protein (SCP) production and Biochemical Oxygen Demand (BOD) removal from whey with mixed yeast culture. For this purpose, 11 yeast strains were isolated from dairy products (M1-M11) and the strains were identified by morphological and physiological properties. These yeast strains were tested for their ability to reduce the BOD and to produce SCP from whey. Among these strains, K. lactis (M2) had the most SCP production from whey with the yield of 11.79 g/l. Ammonium sulphate as nitrogen source had an increasing effect on biomass yield. The mixed culture of the isolated yeast strains with Saccharomyces cerevisiae was used in order to increase the biomass yield and BOD removal. The highest biomass yield (22.38 g/l)... Tipo: Journal article Palavras-chave: Beta-galactosidase; BOD; Candida versatilis; Kluyveromyces lactis; Kluyveromyces marxianus; Mixed culture; SCP; Whey. Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300006 Registros recuperados: 3 Primeira ... 1 ... Última

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