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| Oliveira Neto,M.G.; Santos,H.A.; Fraga,R.E.; Pacheco,A.S.; Sampaio,G.P.; Moura-Costa,L.F.; Meyer,R.; Silva,M. Costa; Trindade,S.C.; Vale,V.L.C.. |
| ABSTRACT This study aimed to evaluate aspects of host immune response using an experimental infection model of Corynebacterium pseudotuberculosis (CP) in C57/Black6 wild-type and knockout for nitric oxide (KO-NO) mice. 28 mice were evaluated: 4 wild-type controls; 10 wild-type infected with CP; 4 KO-NO controls; 10 KO-NO infected with CP. Infection procedures were carried out by intraperitoneal inoculation using 107. Infected C57/Black6 KO-NO mice began to die after the 5° day post-inoculation, up until the 14º day. Neutrophils were found in increased numbers in the infiltrate of KO-NO murine peritoneal cavities. Examination of splenic tissue revealed an accumulation of lymphocytes, predominantly CD8 T-cells, in experimental animal groups. KO-NO animals... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nitric oxid; Knockout mice; Corynebacterium pseudotuberculosis; Caseous limphadenitis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601565 |
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| Hynes,R.O.; Bader,B.L.; Hodivala-Dilke,K.. |
| Many growth factors and their protein kinase receptors play a role in regulating vascular development. In addition, cell adhesion molecules, such as integrins and their ligands in the extracellular matrix, play important roles in the adhesion, migration, proliferation, survival and differentiation of the cells that form the vasculature. Some integrins are known to be regulated by angiogenic growth factors and studies with inhibitors of integrin functions and using strains of mice lacking specific integrins clearly implicate some of these molecules in vasculogenesis and angiogenesis. However, the data are incomplete and sometimes discordant and it is unclear how angiogenic growth factors and integrin-mediated adhesive events cooperate in the diverse cell... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vasculogenesis; Angiogenesis; Integrins; Knockout mice. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500002 |
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| Oliveira,S.C.; Harms,J.S.; Rech,E.L.; Rodarte,R.S.; Bocca,A.L.; Goes,A.M.; Splitter,G.A.. |
| Cellular immune responses are a critical part of the host's defense against intracellular bacterial infections. Immunity to Brucella abortus crucially depends on antigen-specific T cell-mediated activation of macrophages, which are the major effectors of cell-mediated killing of this organism. T lymphocytes that proliferate in response to B. abortus were characterized for phenotype and cytokine activity. Human, murine, and bovine T lymphocytes exhibited a type 1 cytokine profile, suggesting an analogous immune response in these different hosts. In vivo protection afforded by a particular cell type is dependent on the antigen presented and the mechanism of antigen presentation. Studies using MHC class I and class II knockout mice infected with B. abortus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: T cell subsets; Brucella abortus; Knockout mice; Cytokines; Intracellular pathogens; Genetic immunization. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100010 |
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| Liu,Yunqiang; Tao,Dachang; Lu,Yongjie; Yang,Yuan; Ma,Yongxin; Zhang,Sizhong. |
| The mouse testis-enriched Znf230 gene, which encodes a type of RING finger protein, is present primarily in the nuclei of spermatogonia, the acrosome and the tail of spermatozoa. To investigate the role of Znf230 in spermatogenesis, we generated Znf230-deficient mice by disrupting Znf230 exon-5 and exon-6 using homologous recombination. The homozygous Znf230-knockout (KO) mice did not exhibit Znf230 mRNA expression and Znf230 protein production. Znf230 KO mice exhibited no obvious impairment in body growth or fertility. Male Znf230 KO mice had integral reproductive systems and mature sperm that were regular in number and shape. The developmental stages of male germ cells of Znf230 KO mice were also normal. We further examined variations in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Znf230; Knockout mice; Spermatogenesis; Kctd14. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500014 |
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