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| Cachumba,Jorge Javier Muso; Antunes,Felipe Antonio Fernandes; Peres,Guilherme Fernando Dias; Brumano,Larissa Pereira; Santos,Júlio César Dos; Silva,Silvio Silvério Da. |
| ABSTRACT L-asparaginase (EC 3.5.1.1) is an enzyme that catalysis mainly the asparagine hydrolysis in L-aspartic acid and ammonium. This enzyme is presented in different organisms, such as microorganisms, vegetal, and some animals, including certain rodent's serum, but not unveiled in humans. It can be used as important chemotherapeutic agent for the treatment of a variety of lymphoproliferative disorders and lymphomas (particularly acute lymphoblastic leukemia (ALL) and Hodgkin's lymphoma), and has been a pivotal agent in chemotherapy protocols from around 30 years. Also, other important application is in food industry, by using the properties of this enzyme to reduce acrylamide levels in commercial fried foods, maintaining their characteristics (color,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: L-asparaginase; Microbial production; Industrial production; Pharmaceutical application; Acrylamide. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500077 |
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| Amena,S.; Vishalakshi,N.; Prabhakar,M.; Dayanand,A.; Lingappa,K. |
| L-asparaginase is an anti-neoplastic agent used in the lymphoblastic leukaemia chemotherapy. In the present study a novel strain, Streptomyces gulbargensis was explored for the production of extra-cellular L-asparaginase using groundnut cake extract. The optimum pH, temperature, inoculum size and agitation speed for enzyme production were pH 8.5, 40ºC, 1x10(8)spores/ml and 200 rev/min respectively. Maltose (0.5%) and L-asparagine (0.5%) proved to be the best carbon and nitrogen sources respectively. The enzyme was purified 82.12 fold and the apparent molecular weight of the enzyme was found to be 85 kDa. The optima pH and temperature for the enzyme were 9.0 and 40ºC respectively. The enzyme was more stable at the alkaline pH than at the acidic one and it... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: L-asparaginase; Streptomyces gulbargensis; Groundnut cake extract; Optimization; Purification. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100025 |
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