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| Wang,Xiu-Fang; Jin,Xia; Wang,Xiaoyan; Liu,Jing; Feng,Jingjing; Yang,QinQing; Mu,Wenli; Shi,Xiaojuan; Lu,Zhanjun. |
| The retrotransposon known as long interspersed nuclear element-1 (L1) is 6 kb long, although most L1s in mammalian and other eukaryotic cells are truncated. L1 contains two open reading frames, ORF1 and ORF2, that code for an RNA-binding protein and a protein with endonuclease and reverse transcriptase activities, respectively. In this work, we examined the effects of full length L1-ORF2 and ORF2 fragments on green fluorescent protein gene (GFP) expression when inserted into the pEGFP-C1 vector downstream of GFP. All of the ORF2 fragments in sense orientation inhibited GFP expression more than when in antisense orientation, which suggests that small ORF2 fragments contribute to the distinct inhibitory effects of this ORF on gene expression. These results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Green fluorescent protein gene; L1-ORF2; Transcription termination; Orientation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400003 |
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