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	Provedor de dados BJMBR (2) Autor Antunes-Rodrigues,J (1) Borges,B.C. (1) Brunialti,M.K.C. (1) Elias,L.L.K. (1) Fernandes,M.L. (1) Mendes,M.E. (1) Rorato,R. (1) Salomão,R. (1) Mais... Palavra-chave LPS tolerance (2) Astrocytes (1) Ferritin (1) Flagellin (1) Glial fibrillary acidic protein (1) Glutamine synthetase (1) IL-6 (1) Iba-1 (1) MALP-2 (1) Microglia (1) Phagocytosis (1) ROS (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Glial cell activity is maintained during prolonged inflammatory challenge in rats BJMBR Borges,B.C.; Rorato,R.; Antunes-Rodrigues,J; Elias,L.L.K.. We evaluated the expression of glial fibrillary acidic protein (GFAP), glutamine synthetase (GS), ionized calcium binding adaptor protein-1 (Iba-1), and ferritin in rats after single or repeated lipopolysaccharide (LPS) treatment, which is known to induce endotoxin tolerance and glial activation. Male Wistar rats (200-250 g) received ip injections of LPS (100 µg/kg) or saline for 6 days: 6 saline (N = 5), 5 saline + 1 LPS (N = 6) and 6 LPS (N = 6). After the sixth injection, the rats were perfused and the brains were collected for immunohistochemistry. After a single LPS dose, the number of GFAP-positive cells increased in the hypothalamic arcuate nucleus (ARC; 1 LPS: 35.6 ± 1.4 vs control: 23.1 ± 2.5) and hippocampus (1 LPS: 165.0 ± 3.0 vs control: 137.5... Tipo: Info:eu-repo/semantics/article Palavras-chave: LPS tolerance; Astrocytes; Microglia; Glial fibrillary acidic protein; Iba-1; Glutamine synthetase; Ferritin. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800014 Human monocytes tolerant to LPS retain the ability to phagocytose bacteria and generate reactive oxygen species BJMBR Fernandes,M.L.; Mendes,M.E.; Brunialti,M.K.C.; Salomão,R.. Tolerance to lipopolysaccharide (LPS) occurs when animals or cells exposed to LPS become hyporesponsive to a subsequent challenge with LPS. This mechanism is believed to be involved in the down-regulation of cellular responses observed in septic patients. The aim of this investigation was to evaluate LPS-induced monocyte tolerance of healthy volunteers using whole blood. The detection of intracellular IL-6, bacterial phagocytosis and reactive oxygen species (ROS) was determined by flow cytometry, using anti-IL-6-PE, heat-killed Staphylococcus aureus stained with propidium iodide and 2',7'-dichlorofluorescein diacetate, respectively. Monocytes were gated in whole blood by combining FSC and SSC parameters and CD14-positive staining. The exposure to... Tipo: Info:eu-repo/semantics/article Palavras-chave: IL-6; ROS; Phagocytosis; MALP-2; Flagellin; LPS tolerance. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900008 Registros recuperados: 2 Primeira ... 1 ... Última

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