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Probiotics association in the suckling and nursery in piglets challenged with Salmonella typhimurium BABT
Afonso,Esther Ramalho; Parazzi,Larissa José; Marino,Carolina Tobias; Martins,Simone Maria Massami Kitamura; Araújo,Lucio Francelino; Araújo,Cristiane Soares da Silva; Vilela,Flávio Garcia; Moretti,Aníbal de Sant'Anna.
This work aimed to study the probiotics association in 144 piglets from birth to 62 days old. In lactation, the design was completely randomized with two treatments, CTL, 1 mL of distilled water and ProbA, 5g in 15 ml of distilled water, both orally, and in the nursery in randomized block design, with 2x3 factorial arrangement of treatments, ProbA ProbB: 30g/ton of ProbB in the ration; CTL ProbB: 30g/ton of ProbB in the ration; ProbA ProbA, CTL CTL; ProbA CHA (challenged); CTL CHA. At 35 days of age the animal of the nursery were inoculated with Salmonella typhimurium orally. There was no effect of the parameters evaluated during the maternity. In nursery, the feed conversion was favorable to the ProbA. In the evaluation of fecal score, the challenged...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus subtilis; Bifidobacterium pseudolongum; Lactobacillus reuteri; Challenge. Piglets.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200010
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Molecular characterisation and biomass and metabolite production of Lactobacillus reuteri LPB P01-001: a potential probiotic BJM
Pancheniak,Elizete de F. R.; Maziero,Maike T.; Rodriguez-León,José A.; Parada,José L.; Spier,Michele R.; Soccol,Carlos R..
Lactobacillus reuteri LPB P01-001 was isolated from the gastrointestinal tract of wild swine and was characterised by biochemical testing and sequencing of gene 16S rRNA. A simple and low-cost culture medium based on cane sugar (2.5% p/v) and yeast extract (1% p/v) was used in the production of this probiotic. The fermentative conditions were a) pH control at 6.5 and b) no pH control; both were set at 37°C in a 12 L slightly stirred tank bioreactor. Fermentation parameters such as the specific growth rate, productivity and yield of biomass, lactic and acetic acid levels were determined. L. reuteri LPB P01-001 behaves as an aciduric bacteria because it grows better in a low pH medium without pH control. However, the lactic acid production yield was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Probiotic; Lactobacillus reuteri; Molecular characterisation; Fermentation parameters; Lactic and acetic acids.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100015
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Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb BJM
Sepová,H. Kiňová; Dubničková,M; Bilková,A; Bukovský,M; Bezáková,L.
The lactic acid bacterium E isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16S rDNA fragment and species-specific PCR as Lactobacillus reuteri. Its potential antimicrobial activity and ability to modulate immune system in vitro and in vivo was determined. The growth inhibition of potential pathogens decreased from Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica ser. Minnesota to Escherichia coli. The lowest inhibition activity was observed in the case of Candida albicans. The ability of L. reuteri E to modulate biological activities of human and mouse mononuclear cells was estimated in vitro and in vivo, respectively. The production of IL-1β by monocytes in vitro was significantly induced by L. reuteri...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus reuteri; Mononuclear cells; PCR; Antagonistic activity; Phagocytosis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300044
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