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Constitutive nitric oxide synthase (cNOS) activity in Langerhans islets from streptozotocin diabetic rats BJMBR
Fonovich de Schroeder,T.M.; Carattino,M.D.; Frontera,M.; Catanzaro,O.L..
Nitric oxide synthase activity was measured in Langerhans islets isolated from control and streptozotocin diabetic rats. The activity of the enzyme was linear up to 150 µg of protein from control rats and was optimal at 0.1 µM calcium, when it was measured after 45 min of incubation at 37oC in the presence of 200 µM arginine. Specific activity of the enzyme was 25 x 10-4 nmol [3H]citrulline 45 min-1 mg protein-1. Streptozotocin diabetic rats exhibited less enzyme activity both in total pancreas homogenate and in isolated Langerhans islets when compared to control animals. Nitric oxide synthase activity measured in control and diabetic rats 15 days after the last streptozotocin injection in the second group of animals corresponded only to a constitutive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitric oxide synthase; Nitric oxide; Langerhans islets; Streptozotocin; Diabetes.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500004
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Effect on hyperglycemia and pancreas cells of chaya aqueous extracts from two different regions in streptozotocin-induced diabetes rats BJPS
Guzmán,Eduardo Lozano; González,José Cruz Calderón; Flores,Maribel Cervantes; Carrillo,Ada Sandoval; Pescador,María Guadalupe Nieto; Cruz,Francisco Javier Moreno.
Cnidoscolus chayamansa is a native plant of the Mayan region, which is also cultivated in other places like northern Mexico, Tunisia and India. Many properties are attributed to Mayan Chaya, such as aid in the control of glycemia in diabetics. Thus this study aimed to evaluate the hypoglycemic effects of chaya aqueous extracts in a model of streptozotisin-induced diabetic Wistar rats. Chaya aqueous extracts were collected from plants cultivated in Quinta Roo (Mayan region) and Durango (northern Mexico), and in this study we compare their effect with metformin (as a control). Additionally, we compared the extracts mass profiles from both regions by high-resolution liquid chromatography coupled to a triple quadrupole tandem mass detector (HPLC-MS/MS QQQ)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetes; Chaya; Langerhans islets; Hypoglycemia; HPLC.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100619
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