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Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits Ciência Rural
Barbosa,Marcílio Souza; Barbosa,Suzanne Nunes; Nagamoto,Nilson Satoru; Forti,Luiz Carlos; lmeida,Cícero.
ABSTRACT: Atta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ITS; DNA barcoding; Leaf-cutter; Ants.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500150
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