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Registros recuperados: 14
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Effects of plant lectins on in vitro fibroblast proliferation BABT
Sell,Ana Maria; Costa,Celso Paulino da.
Lectins are carbohydrate-binding proteins that have been isolated from various sources and presented a wide spectrum of biological activities. The effects of four lectins, namely, Phaseolus vulgaris phytohemagglutinin, PHA, wheat germ agglutinin, WGA, Artocarpus integrifolia seed lectins, jacalin and artocarpin, on in vitro fibroblasts proliferation were investigated. The lectins did not influence the initial cell adhesion to the plate. PHA and WGA at 10-20 µg/mL concentrations significantly decreased fibroblasts proliferation. At these concentrations, they caused morphological alterations on cells and over 80 µg/mL, promoted cell death. Neither jacalin nor artocarpin significantly affected cell proliferation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lectins; Fibroblasts; Proliferation; MTT.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300006
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Screening for carbohydrate-binding proteins in extracts of Uruguayan plants BJMBR
Plá,A.; Alonso,E.; Batista-Viera,F.; Franco Fraguas,L..
The presence of carbohydrate-binding proteins, namely lectins, ß-galactosidases and amylases, was determined in aqueous extracts of plants collected in Uruguay. Twenty-six extracts were prepared from 15 Uruguayan plants belonging to 12 Phanerogam families. Among them, 18 extracts caused hemagglutination (HAG) that was inhibited by mono- and disaccharides in 13 cases, indicating the presence of lectins. The other 8 extracts did not cause any HAG with the four systems used to detect HAG activity (rabbit and mouse red cells, trypsin-treated rabbit and mouse red cells). For the extracts prepared from Solanum commersonii, HAG activity and HAG inhibition were similar for those prepared from tubers, leaves and fruits, with the chitocompounds being responsible for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phanerogam families; Uruguayan plants; Asialofetuin-Sepharose; Carbohydrate-binding proteins; Lectins; SS-Galactosidases; Amylases.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700005
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Antifungal activity of lectins against yeast of vaginal secretion BJM
Gomes,Bruno Severo; Siqueira,Ana Beatriz Sotero; Maia,Rita de Cássia Carvalho; Giampaoli,Viviana; Teixeira,Edson Holanda; Arruda,Francisco Vassiliepe Sousa; Nascimento,Kyria Santiago do; Lima,Adriana Nunes de; Souza-Motta,Cristina Maria; Cavada,Benildo Sousa; Porto,Ana Lúcia Figueiredo.
Lectins are carbohydrate-binding proteins of non-imune origin. This group of proteins is distributed widely in nature and they have been found in viruses, microorganisms, plants and animals. Lectins of plants have been isolated and characterized according to their chemical, physical-chemical, structural and biological properties. Among their biological activities, we can stress its fungicidal action. It has been previously described the effect of the lectins Dviol, DRL, ConBr and LSL obtained from the seeds of leguminous plants on the growth of yeasts isolated from vaginal secretions. In the present work the experiments were carried out in microtiter plates and the results interpreted by both methods: visual observations and a microplate reader at 530nm....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Sensitivity; Lectins.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200042
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Isolation and characterization of novel lectins from Canavalia ensiformis DC and Dioclea grandiflora Mart. ex Benth. seeds Braz. J. Plant Physiol.
Melgarejo,Luz Marina; Vega,Nohora; Pérez,Gerardo.
Two lectins were isolated from Canavalia ensiformis and Dioclea grandiflora seeds. Gel filtration produced a fraction corresponding to Con A or D. grandiflora lectin while erythroagglutination assays revealed a distinct fraction presenting a lectin that agglutinates human red blood cells (RBCs) but not rabbit RBCs. Hydrophobic interaction chromatography showed that the latter fraction yielded a protein that readily agglutinates human erythrocytes; the lectin was also purified by affinity chromatography on Lac-Sepharose showing similar properties to that of the Phenyl-Sepharose-purified lectin. Despite minor differences (carbohydrate content or A1%1cm), the two lectins showed similar molecular properties in that they consisted of two non-covalently linked...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diocleinae; Leguminoseae; Characterization; Lectins.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000300006
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The importance of heat against antinutritional factors from Chenopodium quinoa seeds Ciênc. Tecnol. Aliment.
Silva,José Antonio da; Pompeu,Dávia Guimarães; Costa,Olavo Flores da; Gonçalves,Daniel Bonoto; Spehar,Carlos Roberto; Marangoni,Sérgio; Granjeiro,Paulo Afonso.
Chenopodium quinoa seeds have high protein content. The nutritional value of quinoa is superior compared with traditional cereals. Its essential amino acid composition is considered next to the ideal, and its quality matches that of milk proteins. In this study, the seed storage proteins from Chenopodium quinoa were extracted, fractionated, partially purified, and characterized. The structural characterization was performed by Tricine-SDS-PAGE and two-dimensional electrophoresis, and it confirmed the presence of proteins of molecular weight of 30 and 7kDa, probably corresponding to lectins and trypsin inhibitors, respectively. The functional characterization of these proteins evidenced their activity as antinutritional factors due to their in vitro...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chenopodium quinoa; Seeds; Lectins; Protease inhibitor; In vitro digestibility.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100074
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Anti-nutritional factors and digestibility of protein in Cayocar brasiliense seeds Ciênc. Tecnol. Aliment.
SILVESTRINI,Virgínia Campos; GONÇALVES,Daniel Bonoto; GRANJEIRO,Paulo Afonso; SILVA,José Antonio da.
Abstract The Brazilian Cerrado presents a wide variety of natural products, including the Pequi (Caryocar brasiliense). The important factor that determines the dietary and nutritional ideality of protein is degestitibility. This work aims to evaluate the protein digestibility of Pequi seeds and the presence of anti-nutritional factors. The protein Pequi almonds were extracted, toasted and untoasted. Evaluation digestibility was structurally characterized by SDS-PAGE 15%, which can be used to analyze simulated gastric fluid (SFG) and digestion in intestinal fluid (SFI) in the presence of pepsin and trypsin/chymotrypsin. The extract from Pequi almonds showed inhibitory activity and was not detected hemagglutination. The intensity of the bands according to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caryocar brasiliense; Almonds; In vitro digestibility; 2S albumin; Peptidase inhibitor; Lectins.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000400632
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Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss) Gayana
Gómez,Leonardo A; Cortés,Raúl; Smith,Carlos T.
This work studied the effect of the lectin phytohaemagglutinin (PHA) and bacterial lipopolysaccharide (LPS) on the activity of nonspecific cytotoxic cells (NCCs) and macrophages of peripheral blood in the rainbow trout. NCCs are cells of the innate immunity, homologues to Natural Killer (NK) cells of mammals, capable of eliminating cells infected with intracellular pathogens and also tumoral cells. Their lytic activity is mediated by the perforin/granzyme system and the Fas receptor-Fas ligand (FasR-FasL) system. Activity of NCCs was evaluated by their capacity to lyse HL-60 tumor cells. On the other hand, macrophages eliminate microorganisms by several mechanisms, including the production of reactive oxygen species (ROS) and nitric oxide (NO). Results...
Tipo: Journal article Palavras-chave: Interleukins; Innate immunity; FasR-FasL; Lectins; Leukocytes.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382013000100007
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Destoxificação da torta de mamona por processos químicos alcalinos em escala semi-industrial e seu uso na alimentação de pequenos ruminantes. Infoteca-e
POMPEU, R. C. F. F.; ARAÚJO, R. A. de; LUCAS, R. C.; NEIVA, J. N. M.; EGITO, A. S. do; CÂNDIDO, M. J. D.; ROGERIO, M. C. P.; PEREIRA, P. L.; XIMENES, L. V.; GALVANI, D. B.; SALLES, H. O..
Resumo: Objetivou-se avaliar a destoxificação da torta de mamona bruta (TMB) por meio de dois produtos alcalinos em diferentes concentrações e seus efeitos sobre a composição química, degradabilidade in situ da MS e o fracionamento de proteínas. Utilizaram-se o hidróxido de cálcio [Ca(OH)2 ] e hidróxido de sódio (NaOH) em duas concentrações (60 g e 90 g), diluídos em quatro quantidades de água (1.000; 1.500; 2.000 e 2.500 ml de água por quilo de TMB). A eficácia dos tratamentos foi avaliada com base na presença das subunidades de ricina em gel a 4,19% de poliacrilamida (SDS-PAGE) em condição desnaturante por meio de análise densitométrica. Já a Ricinus aglutinina foi detectada por meio de bioensaios de hemaglutinação com hemácias de coelho. Observou-se que...
Tipo: Folhetos Palavras-chave: Subprodutos do biodiesel; Hidróxido de cálcio; Ricinnus communis; Caprino; Ovino; Alimento Para Animal; Ricinus Communis; Subproduto; Hidróxido de Sódio; Lectina; Ricina; Nutrição Animal; Alimento Alternativo; Animal nutrition; Sheep feeding; Goats; Biodiesel; Industrial byproducts; Calcium hydroxide; Lectins; Sodium hydroxide.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1117064
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Bonamia ostreae, parasite de l'huître plate, Ostrea edulis: sa position taxonomique parmi les parasites du groupe "microcell", analyses des interactions hôte/parasite chez plusieurs populations d'huîtres plates ArchiMer
Besnard-cochennec, Nathalie.
Bonamia ostreae is the causative agent of hemocyte disease. It has been detected first during mortalities occured in Franc in 1979. Bonamiosis spread rapidly among oysters farming in France and Europ. Another species, Bonamia sp. has been detected in New Zealand and Australia. These paasites were both included in the "microcell" group. This group contains 2 others parasites, Mikrocytos mackini affecting Crassostrea gigas in British Columbia (Canada) and M. roughleyi affecing Saccostrea glomerata in Australia. Structural and molecular caracterisation of all these parasites were realised. Results show tha B. ostreae, B. sp., B. exitiosus. Molecular tools are available for genus detection, Bonamia spp. and Mikrocytos and species identification (B. ostreae, B....
Tipo: Text Palavras-chave: Bonamia ostreae; Ostrea edulis; Taxonomie; Diagnotic; PCR; In situ hybridation; Hémocytes; Lectines; Phagocytose; Résistance; Bonamia ostreae; Ostrea edulis; Taxonomy; Diagnotic; PCR; In situ hybridization; Hemocytes; Lectins; Phagocytosis; Resistance.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00211/32223/30671.pdf
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Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry International Journal of Morphology
Ribeiro Melo-Júnior,Mario; Bezerra Cavalcanti,Carmelita de Lima; Teles de Pontes-Filho,Nicodemos; Carvalho Jr,Luiz Bezerra de; Carneiro Beltrão,Eduardo Isidoro.
This study aims to evalúate the egg-granuloma system in hepatic tissues using lectin histochemistry in experimental Schistosomiasis. Eight Swiss mice were infected with a local strain of Schistosoma mansoni, being submitted forty days later to a perfusión after which slices of liver (4|J.m) were prepared. The tissue samples were incubated with the following peroxidase conjugated lectins: Peanut agglutinin (PNA), Wheat Germ agglutinin (WGA), and Concanavalin A (Con A). All lectins recognized the glycoconjugates in the adult worm tegument. In the hepatic tissue, WGA presented the highest staining followed by PNA and Con A. The PNA presented the most intense staining of the egg-granuloma system while WGA stained the hepatic sinusoid cells and Con A bound...
Tipo: Journal article Palavras-chave: Lectins; Egg-granuloma system; Liver; Schistosoma mansoni.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400030
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Localization of Sugar Residues in the Stomach of Three Species of Monkeys (Tupaiidae glis, Nycticebus cocang and Callithrix jacchus) by Lectin Histochemistry International Journal of Morphology
Fayed,Masoud Hassan; Elnasharty,Mohamed; Shoaib,Mahmoud.
The stomach of three species of non-human primates was investigated by lectin histochemistry to clarify the staining affinity and distribution patterns of their sugar residues. All gastric regions, with little differences between the deep and superficial parts of the same region, were rich in. in N-acetylglucosamine and/or neuraminic acid. Although, the superficial regions of the gastric mucosa were scanty in N-acetylgalactosamine, a- D-glucose and a -D-mannose, the deep parts of the gastric mucosa were rich in these sugars. In conclusion, there is a difference among the mucosubstances of surface and foveolar mucous cells, mucous neck cells, and gastric gland cells. This indicates heterogeneous composition of gastric mucus, or mucus molecules with...
Tipo: Journal article Palavras-chave: Histochemistry; Lectins; Non-human primates; Stomach.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100016
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RECOGNITION OF N-ACETYLGLUCOSAMINE (GLyNAc) AND POLY-N-ACETYLLACTOSAMINE RESIDUES IN VESSELS OF THE RAT PINEAL GLAND International Journal of Morphology
Ferreira-Medeiros,Mildred; Correa-Gillieron,Elenice.
Lectins are proteins with binding sites that recognize a specific sequence of sugar moieties in complex glycoconjugates. In the present study the tomato lectin ­ Lycopersicon esculentum (LEL) (a selective microglial and endothelial marker) has been reported to recognize specific residues of N-acetylglucosamine (GlyNAc) and poly-N-acetyllactosamine. In the pineal gland the biotinylated LEL was used to investigate the appearance of these sugar residues in the structures of the rats during their development and adult life. Our results showed that the binding of LEL occurred exclusively in the material adherents on surface of the endothelia of the vessels in the peripheral and central regions of the gland. An exception can be cited to rats in first postnatal...
Tipo: Journal article Palavras-chave: Pineal; Rat; Lectins; Development; Rosettes; Endothelia.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400008
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A first attempt to elucidate the amino acid sequence of some lichen lectins Phyton
Sacristán,M; Millanes,Am; Fontaniella,B; Legaz,Me; Vicente,C.
Secreted arginases from Evernia prunastri and Xanthoria parietina thalli, exhibiting lectin activity, have been purified to homogeneity. Analyses of both amino acid and glycoside composition are reported. Purified proteins have been subjected to tryptic digestion and some amino acid sequences have been analyzed. An undecapeptide from Evernia arginase, which shows some degree of homology with the domain to which Mn²+ binds, a heptapeptide and an undecapeptide from Xanthoria arginase have been analyzed, and homologies with some arginases from other fungi and plants have been found.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Evernia prunastri; Xanthoria parietina; Arginases; Lectins; Structure analysis.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572008000100021
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ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA Rev. Bras. Frutic.
Serquiz,Alexandre Coelho; Serquiz,Raphael Paschoal; Peixoto,Thayane Kerbele Oliveira das Neves; Santos,Elizeu Antunes dos; Rocha,Hugo Alexandre Oliveira.
ABSTRACT The benefits of various foods, such as fruits and vegetables, have been the focus of several studies aimed at improving welfare, health, and reducing the incidence of diseases. Therefore, the present paper objectified to investigate the presence of molecules of biotechnological and pharmacological interest in peel, seed, and pulp of Annona x atemoya Mabb. Aqueous extracts of the fruit parts were obtained with different buffers and assessed as to their protein and phenolic compounds content. The three parts of the fruit presented different proportions of these compounds when subject to different extraction conditions, with the highest concentrations of proteins being found in the seed and phenolic compounds in the peel of the studied fruit....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Annona x atemoya; Residues; Protease inhibitors; Lectins.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100101
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