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Homogenate extraction of crude polysaccharides from Lentinus edodes and evaluation of the antioxidant activity Ciênc. Tecnol. Aliment.
KE,Leqin; CHEN,Haiyan.
Abstract Crude polysaccharides of Lentinus edodes were extracted using homogenate method. Factors affecting the yield of crude polysaccharides were investigated and optimized by response surface methodology. The homogenate extraction method was compared with traditional heating extraction method. The antioxidant activity of crude polysaccharides from Lentinus edodes was evaluated. Results showed that, the optimal conditions of homogenate extraction were as follows: solvent pH, 10; liquid-solid ratio, 30: 1 (mL: g), extraction time, 66 s; number of extraction, 1. Under these conditions, the yield of crude polysaccharides was (13.2 ± 0.9)%, which was 29.82% higher than that of traditional heating extraction. Crude polysaccharides of Lentinus edodes had good...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crude polysaccharides; Lentinus edodes; Homogenate extraction; Response surface methodology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000300533
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Cadmium determination in Lentinus edodes mushroom species Ciênc. Tecnol. Aliment.
Maihara,Vera Akiko; Moura,Patricia Landim da Costa; Catharino,Marília Gabriela Miranda; Moreira,Edson Gonçalves; Castro,Lilian Pavanelli; Figueira,Rubens Cesar Lopes.
Many studies have drawn attention to the occurrence and concentration of toxic elements found in the fruiting body of mushrooms. Some edible mushroom species are known to accumulate high levels of inorganic contaminants, mainly cadmium, mercury, and lead. There are about 2,000 known edible mushroom species, but only 25 of them are cultivated and used as food. In Brazil, the most marketed and consumed mushroom species are Agaricus bisporus, known as Paris champignon, Lentinus edodes, or Shitake and Pleurotus sp, also called Shimeji or Hiratake. In this study, the concentration of cadmium was determined in Lentinus edodes mushrooms from different cities in São Paulo state and some samples imported from Japan and China. The analyses were performed by graphite...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Mushrooms; Lentinus edodes; Atomic absorption spectrometry.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300019
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Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes Rev. Microbiol.
Correia,Marcos José; Pereira Junior,José Antônio de Sousa; Santos,Jefferson Cunha dos; Cavalcanti,Maria Auxiliadora de Queiroz.
A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes is discussed. Refinements were introduced in a simple method for quantitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by the enzymatic hydrolysis. Modifications in Avicel-dye preparation were enhanced and a spectrophotometer for direct OD measurement in agar test tubes used. The use of a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maximum wavelenght for RBBR (595 nm).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avicel; Cellulase; Remazol Blue; Lentinus edodes; Pleurotus ostreatus.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400010
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