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| Lima,C.C.V.; Ayres,M.C.C.; Pinheiro,R.R.; Costa,J.N.; Andrioli,A.; Souza,T.S.; Azevedo,D.A.A.; Santos,V.W.S.; Araújo,J.F.; Sousa,A.L.M.; Peixoto,R.M.; Damasceno,E.M.; Costa Neto,A.O.. |
| ABSTRACT With the objective of detecting the presence of caprine lentivirus (CLV) in ewe milk and in ram semen, ten matrixes and four reproducers experimentally infected with CLV were used. Samples of ewe milk were collected during the four months of lactation, five collections per animal, totaling 50 samples. Regarding the rams, eight semen collections were made per animal, during one year of experimentation, totaling 32 samples. The milk and semen samples were submitted to DNA extraction and the nested polymerase chain reaction test (nPCR) to detect CLV proviral DNA. Eight (16%) of the milk samples were positive in nPCR originating from two ewes. Only one (3.12%) semen sample was positive. The amplification products were sequenced, and were confirmed to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lentiviruses; Transmission; Cross infection. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200391 |
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| Lima,Carla Caroline Valença de; Ayres,Maria Consuêlo Caribé; Pinheiro,Raymundo Rizaldo; Costa,Joselito Nunes; Souza,Thiago Sampaio de; Pinheiro,Alice Andrioli; Azevedo,Dalva Alana Aragão; Santos,Vanderlan Warlington Souza dos. |
| ABSTRACT: This study was conducted to evaluate caprine arthritis encephalitis virus (CAEV) transmission among sheep using 15 lambs that were distributed in 2 experimental groups. The exposed group consisted of 10 lambs that remained with their mothers, who were experimentally infected with CAEV. The non-exposed group was characterized as the control group and was comprised of 5 lambs that remained with their CAEV-negative mothers. Blood samples were collected monthly from birth until 1 year of life. To evaluate the transmission, an agar gel immunodiffusion test (AGID), enzyme immunoassay (ELISA), immunoblotting (IB), and nested polymerase chain reaction (nPCR) techniques were used. The non-exposed group was negative in all of the tests throughout the whole... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lentiviruses; CAEV; MVV; Intra-species transmission; SRLV. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000450 |
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