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Borges,Graciele Segantini do Nascimento; Goulart,Luiz Ricardo. |
Genotype data of 477 animals of several swine races (Landrace - LD, Large White - LW, Pietrain - PI, LWXLDXPI, Piau, Monteiro, and unknown race) were obtained to determine the allele frequency of the obesity gene. Genotype data of 174 crossbred swine (LWXLDXPI) were also obtained, in order to assess its correlation with carcass evaluation data (lean meat percentage, backfat thickness at P2, loin eye area, adjacent fat area, total fat and meat). Finally, genotype data of 96 pure swine (Landrace, Large White and Pietrain) were collected, to establish its relation with meat quality (drip loss, meat color, texture analysis and intramuscular fat) and carcass evaluation data (lean meat percentage; ham, loin, shoulder and belly weights; and backfat thickness at... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Obese gene; Leptin gene; Quantitative traits. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100007 |
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Choudhary,Vivek; Kumar,Pushpendra; Bhattacharya,Tarun K.; Bhushan,Bharat; Sharma,Arjava. |
Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle. We used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique to screen for DNA polymorphisms of the leptin gene in 403 cattle belonging to various breeds of Bos indicus (Hariana, Sahiwal, Gir and Nimari cattle), Bos taurus (Holstein Friesian (HF) and Jersey cattle) as well as Bos taurus x Bos indicus crossbreds (½ HF x ½ Hariana). In all the cattle we amplified two regions of the leptin gene, a 522 bp fragment comprising the partial intron 2 and exon 3 and another 94 bp fragment consisting of part of exon 2. Digestion of 522 bp PCR products with the BsaAI restriction enzyme revealed three genotypes in... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Leptin gene; PCR-RFLP; Polymorphism; Bos indicus; Bos taurus. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500014 |
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