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	Provedor de dados BABT (3) Electron. J. Biotechnol. (1) Autor Ananthalakshmy,V. K (1) Boiardi,José Luis (1) Gunasekaran,P (1) Gunasekaran,P. (1) Gunasekaran,Paramasamy (1) Gurunathan,Sangiliyandi (1) Kannan,T.R. (1) Molinari,María Laura (1) Raj,K. Chandra (1) Sangiliyandi,G. (1) Mais... Palavra-chave Levansucrase (4) Levan (2) Sucrase (2) Zymomonas mobilis (2) Escherichia coli (1) Expression (1) Extracellular sucrase (1) Gluconic acid (1) In vitro mutagenesis (1) Keto gluconic acids (1) Levans (1) Polymerase (1) Selective modulation (1) Sucrose metabolism (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Journal article (1) Ano 2013 (1) 2004 (1) 1999 (2) País Brazil (3) Chile (1) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Levans production by Gluconacetobacter diazotrophicus Electron. J. Biotechnol. Molinari,María Laura; Boiardi,José Luis. Background: Growth of Gluconacetobacter diazotrophicus with glucose as carbon an energy source has been extensively studied. However, there are no reports in the literature describing growth of G. diazotrophicus in cultures containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. Biomass, levans, gluconic acid and keto gluconic acids production and levansucrase activity were determined in cultures with different sucrose concentration and nitrogen sources. Results: The biomass production was maximal in cultures containing 100 g x L-1 sucrose and inorganic nitrogen. Gluconic acid production was observed under all conditions tested, at levels up to 9 g x L-1 in cultures with sucrose excess and... Tipo: Journal article Palavras-chave: Gluconic acid; Keto gluconic acids; Levans; Levansucrase; Sucrose metabolism. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300007 Separation of levan-formation and sucrose-hydrolysis catalyzed by levansucrase of Zymomonas mobilis using in vitro mutagenesis BABT Sangiliyandi,G.; Kannan,T.R.; Raj,K. Chandra; Gunasekaran,P.. A levansucrase (SacB) of Zymomonas mobilis capable of sucrose hydrolysis but not levan formation was isolated through invitro mutagenesis of cloned sacB gene. When the sacB mutant gene was expressed in Escherichiacoli strains, only 50% of the sucrose-hydrolysing activity (2.0 U/mg) was produced, compared to the wild type levansucrase (4.0 U/mg). Sequencing of the sacB mutant gene revealed changes of two amino acid residues (Phe-102 to Leu and Trp-261 to Lys in the levansucrase). The absence of mutation at the site of Cys of SacB is contradictory to the inhibition kinetics that demonstrated the involvement of Cys in conferring the levan-forming activity to the SacB. The present finding is useful in understanding the mechanism of selective modulation of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Levansucrase; Polymerase; Sucrase; In vitro mutagenesis; Levan; Selective modulation. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400001 Optimization of levan production by Zymomonas mobilis BABT Ananthalakshmy,V. K; Gunasekaran,P. Effect of different fermentation conditions on levan production by Zymomonas mobilis B-4286 was studied. Levan production increased from 5.7-g/l to 12.6-g/l with an increase in initial sucrose concentration (50-150 g/l). Above 15% (20 and 25%) sucrose concentration, there was no increase in the biomass. The sucrose hydrolysis and levan production occurred even in the absence of significant growth of cells. Maximum amount of levan was produced (14.5 g/l) at pH 5 and 15 g /l at 25(0)C temperature. At temperature between 35(0)C and 40(0)C, levan production was not detected. Presence of glucose in the medium considerably reduced levan production (2.8 g/l) than fructose 6.7g/l. Tipo: Info:eu-repo/semantics/article Palavras-chave: Levan; Levansucrase; Sucrase; Zymomonas mobilis. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300004 Differential expression of Zymomonas mobilis sucrase genes (sacB and sacC) in Escherichia coli and sucrase mutants of Zymomonas mobilis BABT Gurunathan,Sangiliyandi; Gunasekaran,Paramasamy. The sacB and sacC genes encoding levansucrase and extracellular sucrase respectively were independently subcloned in pBluescript (high copy number) and in Z. mobilis-E. coli shuttle vector, pZA22 (low copy number). The expression of these genes were compared under identical background of E. coli and Z. mobilis host. The level of sacB gene expression in E. coli was almost ten fold less than the expression of sacC gene, irrespective of the growth medium or the host strain. In Z. mobilis the expression of sacB and sacC genes was shown to be subject to carbon source dependent regulation. The transcript of sacB and sacC was three fold higher in cells grown on sucrose than in cells grown on glucose/fructose. Northern blot analysis revealed that the transcript... Tipo: Info:eu-repo/semantics/article Palavras-chave: Zymomonas mobilis; Escherichia coli; Expression; Levansucrase; Extracellular sucrase. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300001 Registros recuperados: 4 Primeira ... 1 ... Última

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