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Production, purification and characterization of an ionic liquid tolerant cellulase from Bacillus sp. isolated from rice paddy field soil Electron. J. Biotechnol.
Sriariyanun,Malinee; Tantayotai,Prapakorn; Yasurin,Patchanee; Pornwongthong,Peerapong; Cheenkachorn,Kraipat.
Background: Lignocellulosic biomass is a renewable, abundant, and inexpensive resource for biorefining process to produce biofuel and valuable chemicals. To make the process become feasible, it requires the use of both efficient pretreatment and hydrolysis enzymes to generate fermentable sugars. Ionic liquid (IL) pretreatment has been demonstrated to be a promising method to enhance the saccharification of biomass by cellulase enzyme; however, the remaining IL in the hydrolysis buffer strongly inhibits the function of cellulase. This study aimed to isolate a potential IL-tolerant cellulase producing bacterium to be applied in biorefining process. Result: One Bacillus sp., MSL2 strain, obtained from rice paddy field soil was isolated based on screening of...
Tipo: Journal article Palavras-chave: Biorefinery; Cellulase; Ionic liquid; Lignocellulosic biomass.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100004
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Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene Electron. J. Biotechnol.
Ferreira,Verônica; Faber,Mariana de Oliveira; Mesquita,Sabrina da Silva; Pereira Jr,Nei.
In Brazil, the production of ethanol from sugarcane produces large amounts of lignocellulosic residues (bagasse and straw), which have been driving research and development for the production of second generation ethanol. In the present work, a recombinant Saccharomyces cerevisiae strain expressing the β-glucosidase gene from Humicola grisea was used for ethanol production from three different cellulosic sources by simultaneous saccharification and fermentation. Initially, a enzymatic pre-hydrolysis step was done with a solid:liquid ratio of 1:4, and an enzymatic load of 25 filter paper activity (FPU).g-1 of cellulosic substrate. Using sugarcane bagasse pretreated cellulignin, crystalline cellulose and carboxymethyl cellulose, 51.7 g L-1, 41.7 g...
Tipo: Journal article Palavras-chave: Bioethanol; Lignocellulosic biomass; Sugarcane bagasse derived cellulignin.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200005
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Thermotolerant fermenting yeasts for simultaneous saccharification fermentation of lignocellulosic biomass Electron. J. Biotechnol.
Choudhary,Jairam; Singh,Surender; Nain,Lata.
Lignocellulosic biomass is the most abundant renewable source of energy that has been widely explored as second-generation biofuel feedstock. Despite more than four decades of research, the process of ethanol production from lignocellulosic (LC) biomass remains economically unfeasible. This is due to the high cost of enzymes, end-product inhibition of enzymes, and the need for cost-intensive inputs associated with a separate hydrolysis and fermentation (SHF) process. Thermotolerant yeast strains that can undergo fermentation at temperatures above 40°C are suitable alternatives for developing the simultaneous saccharification and fermentation (SSF) process to overcome the limitations of SHF. This review describes the various approaches to screen and develop...
Tipo: Journal article Palavras-chave: Bioethanol; Biofuel; Enzymes; Genome shuffling; Lignocellulosic biomass.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300013
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Degradação catalítica de lignina kraft para determinação do potencial antioxidante para fins industriais. Infoteca-e
VAZ JUNIOR, S.; MATIAS, A. E. B.; SAMPAIO, B. L..
Resumo: A biomassa lignocelulósica apresenta-se como uma fonte promissora de matéria-prima renovável que, por meio dos processos de conversão, leva à obtenção de produtos de alto valor agregado, como derivados fenólicos. O presente trabalho apresenta um processo de conversão catalítica da lignina Kraft a produtos com potencial aplicação como agentes antioxidantes. As reações de hidrogenólise foram realizadas utilizando como solventes orgânicos metanol, etanol, isopropanol ou isopropanol:ácido fórmico (1:1 v/v). Para cada solvente, quatro catalisadores de metais de transição foram testados (Pd/CaCO3, Pd/C, Ru/C e In). Através das análises por UHPLC-ESI(+)--MS foram identificadas 21 substâncias nas frações isoladas, com composição predominantemente fenólica....
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Química verde; Catálise heterogênea; Biomassa lignocelulósica; Lignina Kraft; Lignocellulosic biomass; Heterogeneous catalysis; Biomassa; Lignina; Green chemistry.
Ano: 2021 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1134129
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Feedstock and inoculum characteristics and process parameters as predictors for methane yield in mesophilic solid-state anaerobic digestion Anais da ABC (AABC)
NIQUINI,GABRIELA R.; SILVA,SUZIMARA R.; COSTA JUNIOR,ESLY F.; COSTA,ANDRÉA O.S..
Abstract: In this study, several linear regression models were tested to predict the cumulative 30-day methane yield produced in mesophilic solid-state anaerobic digestion, employing diverse lignocellulosic biomass sources. Data collected from 13 studies were utilized, totalizing 86 experimental points, divided into regression and validation. Models containing higher order terms, the inverse of variables and interactions among all eleven input variables were tested. Simple linear models utilizing a single variable were unable to describe the methane production, giving an R² lower than 0.37. However, combinations of multiple variables and its inverses as only independent variable permitted an increase in simple linear models predictive capacity up to 63% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lignocellulosic biomass; Linear regression; Methane; Polynomial models; Solid-state anaerobic digestion.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700865
Registros recuperados: 5
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