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Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum Anais da ABC (AABC)
LLERENA,JUAN P.P.; ARAÚJO,PEDRO; MAZZAFERA,PAULO.
ABSTRACT Saccharum spontaneum has been used for the development of energy cane a crop aimed to be used for the production of second-generation ethanol, or lignocellulosic ethanol. Lignin is a main challenge in the conversion of cell wall sugars into ethanol. In our studies to isolate the genes the lignin biosynthesis in S. spontaneum we have had great difficulty in RT-PCR reactions. Thus, we evaluated the effectiveness of different additives in the amplification of these genes. While COMT and CCoAOMT genes did not need any additives for other genes there was no amplification (HCT, F5H, 4CL and CCR) or the yield was very low (CAD and C4H). The application of supplementary cDNA was enough to overcome the non-specificity and low yield for C4H and C3H, while...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomass; Cell wall; Energy cane; Lignin; Lignocellulosic ethanol; Sugarcane.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100509
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Ethanol from lignocellulosic biomass Ciencia e Investigación Agraria
Abril,Diana; Abril,Alejandro.
Ethanol is the liquid combustible that has become the most promising alternative substitute for gasoline because of the experience gained in its production, the possibility of mixing it with gasoline in different proportions, the possibility of using the existing gasoline distribution infrastructure, and the fact that major changes in engines are not required for its use. Lignocelluloses offer great potential as a biomass source for ethanol production, although their use still requires in-depth analysis with an objective and holistic focus that includes present and future technologic implications. The present article reviews current knowledge about the characteristics and sources of vegetable biomass, as well as the development and possibilities for...
Tipo: Journal article Palavras-chave: Alcoholic fermentation; Energy; Ethanol; Lignocellulosic ethanol; Vegetable biomass.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202009000200003
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