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Palavra-chave Lipase (51) Protease (7) Amylase (5) Esterification (4) Immobilization (3) Optimization (3) Trypsin (3) Aspergillus niger (2) Characterization (2) Hydrolysis (2) Mutation (2) Pepsin (2) Pseudomonas (2) Purification (2) Solid-state fermentation (2) Yarrowia lipolytica (2) Agglutinin (1) Agricultural residues (1) Agroindustrial residues (1) Albumin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (42) Journal article (7) Text (1) Ano 2020 (2) 2018 (1) 2017 (3) 2016 (5) 2015 (3) 2014 (5) 2013 (6) 2012 (1) 2011 (2) 2010 (2) 2009 (4) 2008 (2) 2007 (3) 2006 (5) 2005 (2) 2004 (1) 2002 (2) 2001 (1) 2000 (1) Mais... País Brazil (42) Chile (7) France (1) Japan (1) Idioma Inglês (43) Português (7) Japonês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 51 Primeira ... 1 2 3 ... Última Influence of the pH of glutaraldehyde and the use of dextran aldehyde on the preparation of cross-linked enzyme aggregates (CLEAs) of lipase from Burkholderia cepacia Electron. J. Biotechnol. Caballero Valdés,Eduardo; Wilson Soto,Lorena; Aroca Arcaya,Germán. The preparation of cross-linked enzyme aggregates (CLEAs) of lipase has been a challenge due the low amount of lysine residues that lipases have on their surface. The results show that CLEAs prepared using dextran aldehyde (100-200KDa) have a higher hydrolysis activity and particle size (activities between 3186 ± 21 U/g of CLEA and 4800 ± 30 U/g of CLEA and particle sizes between 52.6 ± 18.7 µm and 126.2 ± 53.5 µm) than CLEAs prepared with glutaraldehyde (0.1 KDa) (activities between 894 ± 16 U/g of CLEA and 2874 ± 20 U/g of CLEA and particle sizes between 21.2 ± 5.1 µm and 83.4 ± 24.9 µm); Thermal stability assays of bioctalysts at 60ºC at pH 7.0 using phosphate buffer 25 mM showed that CLEAs prepared with dextran aldehyde have lower residual... Tipo: Journal article Palavras-chave: Albumin; Dextran aldehyde; Glutaraldehyde; Immobilisation; Lipase. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300010 Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica Electron. J. Biotechnol. Papanikolaou,Seraphim; Chevalot,Isabelle; Galiotou-Panayotou,Maria; Komaitis,Michael; Marc,Ivan; Aggelis,George. The aim of the present study was to assess the potential of valorisation of a solid industrial derivative of tallow, composed of saturated free-fatty acids ("stearin"), by fermentations carried out by the yeast Yarrowia lipolytica ACA-DC 50109 in order to produce microbial lipid, biomass and extra-cellular lipase. High quantities of biomass were produced (biomass yield of around 1.1 ± 0.1 g of total biomass produced per g of fat consumed) when the organism was grown in shake flasks, regardless of the concentration of extra-cellular nitrogen present. Cellular lipids accumulated in notable quantities regardless of the nitrogen availability of the medium, though this process was clearly favoured at high initial fat and low initial nitrogen concentrations. The... Tipo: Journal article Palavras-chave: Lipase; Microbial lipids; Single-cell protein; Tallow; Yarrowia lipolytica. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300010 Effect of methyl branching of C8H18 alkanes and water activity on lipase-catalyzed enantioselective esterification of ibuprofen Electron. J. Biotechnol. Gottemukkala,Vijay V; Saripella,Kalyan K; Kadari,Anil K; Neau,Steven H. The purpose of this research was to study the effect of the methyl branching of a high log P alkane solvent and the water activity in the organic medium on the initial rate and the enantioselectivity of ibuprofen esterification catalyzed by Candida rugosa lipase. Resolution of ibuprofen is important because S-(+)-ibuprofen has the desired pharmacological activity, whereas the R-(-)-enantiomer causes much of the side effects. The Candida rugosa lipase-catalyzed reaction in isooctane at 40ºC and 0.73 water activity gave the best results, both in terms of the initial reaction rate and the enantioselectivity of the reaction. An increase in water activity allowed a higher reaction rate and enantiomeric excess in each of the four solvents. An increase in methyl... Tipo: Journal article Palavras-chave: Enantioselective; Esterification; Ibuprofen; Lipase; Solvent-effect; Water activity. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100002 Yeast lipases: enzyme purification, biochemical properties and gene cloning Electron. J. Biotechnol. Vakhlu,Jyoti. Lipases are placed only after proteases and carbohydrases in world enzyme market and share about 5% of enzyme market. They occur in plants, animals and microorganisms and are accordingly classified as plant, animal and microbial lipases. Wherever they exist, they function to catalyze hydrolysis of triglycerides to glycerol and fatty acid. Like carbohydrases and proteases, lipases of microbial origin enjoy greater industrial importance as they are more stable (compared to plant and animal lipases) and can be obtained in bulk at low cost. Majority of yeast lipases are extracelluar, monomericglycoproteins with molecular weight ranging between ~33 to ~65 kD. More than 50% reported lipases producing yeast, produce it in the forms of various isozymes. These... Tipo: Journal article Palavras-chave: Candida; Geotrichum; Lipase; Trichosporon; Yeast. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100010 Immobilization and stability studies of a lipase from thermophilic Bacillus sp: The effect of process parameters on immobilization of enzyme Electron. J. Biotechnol. Nawani,Neerupma; Singh,Rajvinder; Kaur,Jagdeep. A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60&ordm;C and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30% v/v each). The activity (assayed by determining the release of pNP from pNP laurate) was stimulated up to 60% of these solvents in enzyme reaction mixture. The catalytic properties of this thermostable enzyme can be further improved via the use of different immobilization techniques and reaction conditions. Enzyme was immobilized on different solid supports and their enzyme activity and stability was compared. The enzyme was adsorbed on silica and HP-20 beads followed by cross-linking with gluteraldehyde on HP-20,... Tipo: Journal article Palavras-chave: Esterification; Immobilization; Lipase; Thermostability. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500011 Comparative esterification of phenylpropanoids versus hydrophenylpropanoids acids catalyzed by lipase in organic solvent media Electron. J. Biotechnol. Cassani,Julia; Luna,Héctor; Navarro,Arturo; Castillo,Edmundo. The esterification of phenylpropanoid and hydrophenylpropanoid acids, catalyzed by candida antarctica lipase B (CAL-B), with several alcohols has demonstrated that the substitution pattern on the aromatic ring has a very significant influence on the reactivity of the carboxyl group due, mainly, to electronic effects, when compared to the unsaturated acids with the hydrogenated acids. It is also clear that in the saturated acids there still remain some unclear effects related to the aromatic substituents. Tipo: Journal article Palavras-chave: CAL-B; Electronic effects; Esterification; Lipase; Phenylpropanoid acids. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400004 Thermostable lipase from a newly isolated Staphylococcus xylosus strain; process optimization and characterization using RSM and ANN Electron. J. Biotechnol. Khoramnia,Anahita; Lai,Oi Ming; Ebrahimpour,Afshin; Tanduba,Carynn Josue; Voon,Tan Siow; Mukhlis,Suriati. Normal feed forward back-propagation artificial neural network (ANN) and cubic backward elimination response surface methodology (RSM) were used to build a predictive model of the combined effects and optimization of culture parameters for the lipase production of a newly isolated Staphylococcus xylosus. The results demonstrated a high predictive accuracy of artificial neural network compared to response surface methodology. The optimum operating condition obtained from the ANN model was found to be at 30ºC incubation temperature, pH 7.5, 60 hrs incubation period, 1.8% inoculum size and 60 rpm agitation. The lipase production increased 3.5 fold for optimal medium. The produced enzyme was characterized biochemically and this is the first report about a... Tipo: Journal article Palavras-chave: Artificial neural network; Characterization; Lipase; Optimization; Response surface methodology; Staphylococcus xylosus. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500015 Analysis of castor bean ribosome-inactivating proteins and their gene expression during seed development Genet. Mol. Biol. Loss-Morais,Guilherme; Turchetto-Zolet,Andreia Carina; Etges,Matheus; Cagliari,Alexandro; Körbes,Ana Paula; Maraschin,Felipe dos Santos; Margis-Pinheiro,Márcia; Margis,Rogério. Ribosome-inactivating proteins (RIPs) are enzymes that inhibit protein synthesis after depurination of a specific adenine in rRNA. The RIP family members are classified as type I RIPs that contain an RNA-N-glycosidase domain and type II RIPs that contain a lectin domain (B chain) in addition to the glycosidase domain (A chain). In this work, we identified 30 new plant RIPs and characterized 18 Ricinus communis RIPs. Phylogenetic and functional divergence analyses indicated that the emergence of type I and II RIPs probably occurred before the monocot/eudicot split. We also report the expression profiles of 18 castor bean genes, including those for ricin and agglutinin, in five seed stages as assessed by quantitative PCR. Ricin and agglutinin were the most... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agglutinin; Evolution; Lipase; Ricinus communis; Ricin; RIPs. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100011 Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development ArchiMer Tovar Ramirez, Dariel; Zambonino-infante, Jose-luis; Cahu, Chantal; Gatesoupe, Joel; Vazquez Juarez, R. Recent results have demonstrated that live yeast in diets improved gut maturation in sea bass larvae. In this study, we tested the absence and two levels of live yeast in sea bass larvae diets. Specimens were fed from time of mouth opening to 37 days after hatching a diet of 0%, 1.1%, or 5.7% wet weight of live yeast (Debaryomyces hansenii CBS 8339). Yeast incorporation improved survival 10%, and reduced malformed larvae. In groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group. Final mean weight in groups fed 1.1% yeast was twice that of other groups. Activities and concentrations of mRNA trypsin and lipase were higher in the two groups fed yeast than in the control group, whereas activity and concentration of mRNA... Tipo: Text Palavras-chave: Polyamines; Lipase; Trypsin; Amylase; Enzymes; Gut maturation; Fish larvae. Ano: 2004 URL: https://archimer.ifremer.fr/doc/2004/publication-831.pdf 乳脂肪およびその分別物のリパーゼ分解 OAK 丹治, 幹男; 大西, 正男; 司城, 不二; Tanji, Mikio; Ohnishi, Masao; Tsukasaki, Fuji. 微生物起源の市販リパーゼを用いて、天然のバターフレーバーを強化する目的で乳脂肪を分解した。 1）市販酵素であるパラターゼAとリパーゼFを比較すると、 リパーゼFの方が分解率が高く、フレーバーも良好であった。 また、酵素添加量を増やすことにより短時間で分解率を上げることができた。 2）リパーゼFでバターオイルを分解した壌合、水相量の違い（10％と20％）、水道水の使用および水酸化物添加による加水分解率への影響はほとんどなかった。 3）リパーゼ分解により遊離した脂肪酸としては11種が検出され、そのうち主なものはパルミチン酸、ステアリン酸およびオレイン酸であった。24hと48hの分解時間で比較すると、遊離した脂肪酸量には違いがあったが、その組成はほとんど変わらなかった。またショートニング希釈およびマドレーヌでの実用試験においても、分解時間によるフレーバーの違いは単に強度的なものだけであった。 4）乳脂肪から分別された低融点画分を同様にリパーゼで分解すると、分解率はバターオイルの場合と比べて多少低かったが、遊離した短・中鎖の脂肪酸組成には差がなかった。官能試験では、刺激臭が弱くなり風味的にマイルドで、またマドレーヌでの実用試験にいでもフレーバー改善効果が認められた。しかし、これはバターフレーバー成分が分別の過程で低融点性画分に多く移行したことによると判断された。 Palavras-chave: バター; 乳脂肪; リパーゼ; 加水分解; フレーバー; Butter; Milk fat; Lipase; Hydrolysis; Flavor. Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1251 Enzimas digestivas exógenas na alimentação de juvenis de tambaqui PAB Nunes,Érica da Silva Santiago; Cavero,Bruno Adan Sagratzki; Pereira-Filho,Manoel; Roubach,Rodrigo. O objetivo deste trabalho foi avaliar o efeito da adição na ração das enzimas digestivas exógenas amilase, lipase e protease sobre o desempenho de tambaqui, em três experimentos independentes. Em cada experimento foi avaliado o efeito da inclusão de uma enzima na ração em diferentes porcentagens: 0,0, 0,05, 0,1 e 0,2%, cada uma com quatro repetições, com 28 dias de duração. Foram realizadas biometrias do peso inicial, para homogeneização da amostra, e do peso final a fim de verificar o efeito dos tratamentos sobre o desempenho dos peixes. Em cada unidade experimental foram estocados dez peixes, alimentados duas vezes ao dia (8 e 14h) até a saciedade aparente, com ração comercial extrusada com 28% de proteína bruta, triturada, adicionada de enzima e depois... Tipo: Info:eu-repo/semantics/article Palavras-chave: Colossoma macropomum; Piscicultura; Nutrição de peixes; Amilase; Lipase; Protease. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000100019 Effects of Lysophospholipid and Lipase Enzyme Supplementation to Low Metabolizable Energy Diets on Growth Performance, Intestinal Morphology and Microbial Population and Some Blood Metabolites in Broiler Chickens Rev. Bras. Ciênc. Avic. Movagharnejad,M; Kazemi-Fard,M; Rezaei,M; Teimuri-Yansari,A. ABSTRACT This experiment aimed to evaluate the impacts of dietary lysophospholipid (LPL) and lipase enzyme complementation based on low-energy diets on growth performance, intestinal morphology, blood metabolites, immune response, and carcass traits in broiler chickens. Two hundreds broiler chicks were assigned to a completely randomized design with five treatments and four replications with ten one-day old chicks. The five treatments were: positive control (PC) without LPL supplementation and adequate in all nutrients, negative control (NC) without LPL the reduced 150 kcal/kg of metabolizable energy, NC+ 0.15% LPL (LPL15), NC+ lipase (NCL), NC+ 0.15% LPL+ lipase (NCLL). Feeding LPL improved body weight gain and feed conversion ratio (FCR). In contrast,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler Chicken; Lipase; Metabolizable Energy; Growth Performance; Intestinal Morphology. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200316 Composition of gastrointestinal content, protease and lipase activities in summer and winter of four freshwater siluriforms (Teleostei: Actinopterygii) with two different feeding habits Rev. Bras. Zool. Almeida,Ana Paula Gottlieb; Zardo,Everton Luis; Toni,Candida; Behr,Everton Rodolfo; Silva,Leila Picolli da; Vieira,João Paes; Loro,Vania Lúcia; Baldisserotto,Bernardo. ABSTRACT The aim of this study was to determine the composition of gastrointestinal content and protease and lipase activities in summer and winter as well as to evaluate the relationship between digestive enzyme activity and centesimal composition of gastrointestinal content and feeding habits of two omnivorous species, Rhamdia quelen (Quoy & Gaimard, 1824) and Pimelodus maculatus (LaCèpede, 1803), and of two detritivorous species, Loricariichthys anus (Valenciennes, 1835) and Hypostomus commersoni (Valenciennes, 1836). The activities of pepsin, trypsin, chymotrypsin, and lipase, and the levels of proteins and lipids in the gastrointestinal tract, were evaluated. The enzyme activities were not related to the centesimal composition of gastrointestinal... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chymotrypsin; Fish; Lipase; Pepsin; Trypsin. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100322 Inclusion of exogenous enzymes to feathers during processing on the digestible energy content of feather meal for adult dogs R. Bras. Zootec. Pacheco,Gabriel Faria Estivallet; Pezzali,Júlia Guazzelli; Kessler,Alexandre de Mello; Trevizan,Luciano. ABSTRACT This study was conducted to determine the coefficient of total tract apparent digestibility (CTTAD) of nutrients and gross energy (GE), metabolizable energy (ME), coefficient of metabolizability of gross energy (CM of GE), and fecal characteristics of dogs fed diets with two levels of feather meal, with or without addition of an enzyme blend containing lipase and protease activity. Ten adult Beagle dogs (aged between 1 and 2 years and weighing 10.2±1.4 kg) were arranged in a 5 × 3 incomplete Latin square design with five treatments and three periods. The extruded basal diet was provided in equal amounts to all dogs (220 g/d), and 7.5 and 15% of hydrolyzed feather meal without enzymes (HFM) or HFM processed with addition of the enzyme blend (HFM... Tipo: Info:eu-repo/semantics/article Palavras-chave: By-product; Digestibility; Lipase; Protease; Protein source. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000600288 Efeito da suplementação enzimática em rações com farelo de soja e soja integral extrusada sobre a digestibilidade de nutrientes, o fluxo de nutrientes na digesta ileal e o desempenho de frangos R. Bras. Zootec. Garcia,Elis Regina de Moraes; Murakami,Alice Eiko; Branco,Antonio Ferriani; Furlan,Antonio Claudio; Moreira,Ivan. Três experimentos foram realizados com o objetivo de avaliar o efeito da suplementação enzimática em rações à base de milho e soja sobre o desempenho (Experimento 1), a digestibilidade de nutrientes (Experimento 2) e o desempenho e o fluxo de nutrientes na digesta ileal (Experimento 3) em frangos de corte. No Experimento 1, para o cálculo das rações experimentais, a composição química do farelo de soja (FS) e da soja integral extrusada (SIE) foi superestimada em 7% para os valores de EM e PB e 5% para Met, Met+Cis e Lis (Trat. C, D e E) e 9% para EM, 7% para PB e 5% para Met, Met+Cis e Lis (Trat. F, G e H), quando houve a adição de enzimas (Allzyme Vegpro<FONT FACE=Symbol>â</FONT> e Allzyme Lipase<FONT FACE=Symbol>â</FONT>).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Complexo multienzimático; Farelo de soja; Frango de corte; Lipase; Soja integral extrusada. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982000000500020 Assessment of lipolytic activity of isolated microorganisms from the savannah of the Tocantins Biological Sciences Soares, Marysa de Kássia Guedes; Facundes, Bruna Coelho; Chagas Júnior, Aloísio Freitas; Silva, Ezequiel Marcelino da. Current study assesses the biodiversity and selects lipase-producer microorganisms with industrial interest, from the savannah of the state of Tocantins, Brazil. Seventeen pequi microorganisms (Caryocar brasiliense) were isolated in the decomposition stage and 35 microorganisms were retrieved from the soil fraction under the collected pequi. Yarrowia lypolitica strain was used as positive control in all assays. The 52 strains were subjected to tests in a solid medium with Tween 20 for checking halos formed by crystals, indicating lipase production by inoculated strains. Another test to confirm lipase producers was conducted in microplates with liquid medium and enriched with p-nitrophenyl palmitate (pNPP) monitored at 410 ηm. The AS16 and AP5 strains... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Caryocar brasiliense; Yarrowia lypolitica; P-nitropheny lpalmitate. Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/24324 Aqueous extract of Psidium guajava leaves: phenolic compounds and inhibitory potential on digestive enzymes Anais da ABC (AABC) SIMÃO,ANDERSON A.; MARQUES,TAMARA R.; MARCUSSI,SILVANA; CORRÊA,ANGELITA D.. ABSTRACT Leaves of Psidium guajava L. (guava) have been widely used in the popular way for prevention and treatment of various diseases. Thus, the objective of this study was to evaluate the inhibitory potential of leaves aqueous extract from three cultivars of P. guajava (Pedro Sato, Paluma and Século XXI) on α-amylase, α-glycosidase, lipase, and trypsin enzymes, in the presence or not of simulated gastric fluid and to determine the content of phenolic compounds by high performance liquid chromatography. All cultivars presented the same composition in phenolic compounds, but in different proportions. The compounds identified are gallic acid, epigallocatechin gallate, syringic acid, o-coumaric acid, resveratrol, quercetin, and catechin (which was the major... Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plants; Obesity; Α-amylase; Α-glycosidase; Lipase; Trypsin. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502155 Atividade lipolítica do leite com células somáticas ajustadas para diferentes níveis Arq. Bras. Med. Vet. Zootec. Santos,M.V.; Oliveira,C.A.F.; Augusto,L.F.B.; Aquino,A.A.. Estimou-se a origem da atividade lipolítica do leite com contagens de células somáticas (CCS) ajustadas para diferentes níveis. A partir de lotes de leite cru com baixa (100.000 cel/ml) e alta (1.000.000 cel/ml) CCS, formaram-se três tratamentos: A) leite com baixa CCS; B) leite com alta CCS; C) leite originalmente com baixa CCS e com adição de células somáticas. O leite foi submetido à pasteurização e armazenado por 21 dias sob refrigeração a 6ºC. Durante o período de armazenamento, foram coletadas amostras nos dias 1, 7, 14 e 21 para avaliação da concentração de ácidos graxos livres (AGL). Não foi observado efeito de tratamento sobre a concentração de AGL, durante todo o período de armazenamento e nem em cada dia de coleta isoladamente, cujas médias de... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leite; Células somáticas; Atividade enzimática; Lipase. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400002 Efeitos do período de jejum entre o nascimento e o alojamento de pintos de corte e da adição de óleo à ração sobre o desenvolvimento do trato gastrintestinal e concentração de lipase Arq. Bras. Med. Vet. Zootec. Cançado,S.V.; Baião,N.C.. Os objetivos deste trabalho foram determinar o efeito de três períodos de jejum (zero, 24 e 48 horas) entre o nascimento e o alojamento de pintos e da adição de óleo à ração sobre o crescimento alométrico dos órgãos digestivos e estudar a atividade da lipase no pâncreas e intestino delgado. Foram utilizados 720 pintos de corte machos, criados de um a 15 dias de alojamento. As avaliações do crescimento dos órgãos digestivos foram feitas nos dias zero, três, seis, nove, 12 e 15 de idade. As avaliações da concentração de lipase no pâncreas foram feitas nos dias zero, três, seis, nove, 12 e 15, e no intestino delgado nos dias zero, três e seis. O delineamento experimental foi em arranjo fatorial 3 x 2 (três períodos de jejum e dois tipos de ração). O... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pintos de corte; Trato gastrintestinal; Lipase; Jejum. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000600011 Reatividade in vitro de lipase submetida a diferentes tratamentos tecnológicos Arq. Bras. Med. Vet. Zootec. Ferreira,V.P.A.; Ferreira,W.M.; E.O.S.,Saliba; Teixeira,A.O.. Analisou-se a atividade enzimática in vitro de uma lipase em diferentes condições de temperatura (simulando o processo de peletização) e pH (acidez gástrica) e na presença de íons minerais e metálicos. Para avaliar a estabilidade térmica, a enzima foi incubada a 80(0)C por quatro tempos (0, 5, 10 e 15 minutos). O efeito do pH foi estudado submetendo-se a enzima a quatro valores de pH (7,3, 2,0, 3,2 e 5,0). Para avaliar o efeito da lipase na presença de íons minerais e metálicos, incubou-se a enzima em diferentes soluções contendo cálcio, cobre, cobalto, manganês e ferro. Frente às diferentes condições, a lipase conservou atividade parcial, mas não pôde ser considerada uma enzima ideal em função do efeito inibidor promovido pelo pH ácido e pelas soluções... Tipo: Info:eu-repo/semantics/article Palavras-chave: Atividade; Lipase; Íons; PH; Temperatura. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300011 Registros recuperados: 51 Primeira ... 1 2 3 ... Última

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