Aurantiochytrium mangrovei Sk-02 was grown in a medium containing glucose (40 g/l), yeast extract (10 g/L) and sea salts (15 g/L) at temperatures ranging from 12 to 35°C. The fastest growth (µmax= 0.15 h-1) and highest fatty acid content of 415 mg/g-dry cell weight were found in the cells grown at 30°C. However, the cells grown at 12°C showed the highest percentage of polyunsaturated fatty acid (PUFA) (48.6% of total fatty acid). The percentage of docosahexaenoic acid (DHA) and pentadecanoic acid (C15:0) decreased with an increase in the growth temperature, whereas, palmitic acid (C16:0), stearic acid (C18:0) and DPA (C22:5n6) increased with an increase in the growth temperature. The composition of the major lipid class (%w/w) was slightly affected by the...
Total lipid content and lipid class composition were analyzed in gonads, liver and white muscle of yellowfin tuna (Thunnus albacares) throughout ovary development to understand its reproductive allocation strategy and to assess the relation between female condition and reproduction. A total of 112 females were collected onboard purse-seiner in the Western Indian Ocean from January to March 2009, from June to July 2009, and from April to May 2010. Gonads were characterized by highly variable total lipid contents ranging from 5 to 27 μg mg−1 of wet weight (ww) with a predominance of neutral lipids, mainly triacylglycerols (TAG) and sterol- and wax-esters. The different lipid classes in gonads described an accumulative pattern through the maturity process...
