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| Suganuma, Keisuke; Yamasaki, Shino; Molefe, Nthatisi Innocentia; Musinguzi, Peter Simon; Davaasuren, Batdorj; Mossaad, Ehab; Narantsatsral, Sandagdorj; Battur, Banzragch; Battsetseg, Badgar; Inoue, Noboru. |
| Dourine is caused by Trypanosoma equiperdum via coitus with an infected horse. Although dourine is distributed in Equidae worldwide and is listed as an internationally important animal disease by the World Organization for Animal Health (OIE), no effective treatment strategies have been established. In addition, there are no reports on drug discovery, because no drug screening system exists for this parasite. A new T.?equiperdum strain was recently isolated from the genital organ of a stallion that showed typical symptoms of dourine. In the present study, we adapted T.?equiperdum IVM-t1 from soft agarose media to HMI-9 liquid media to develop a drug screening assay for T.?equiperdum. An intracellular ATP-based luciferase assay using CellTiter-Glo reagent... |
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Palavras-chave: Colorimetric assay; Drug screening system; Liquid culture; Luciferase assay; Trypanosoma equiperdum. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4538 |
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| ZHANG,XIN; MA,YAN-NI; ZHANG,JUN-WU. |
| In vitro suspension culture procedures for erythroid progenitor cells make it possible for us to obtain large cultures of erythrocyte populations for the investigation of globin gene switching. In this study we aimed to establish optimized culture systems for neonatal and adult erythroblasts and to explore the globin expression patterns in these culture systems. To culture CD34+ cells purified from human umbilical cord blood (CB) and adult bone marrow (BM), we respectively replaced the fetal bovine serum (FBS) with human cord serum and human adult serum. These CD34+ cells were then induced to erythroid differentiation. All the globin mRNA (including á-, ξ-, β-, γ-and ε-globin), the hemoglobin (Hb)-producing erythroid... |
| Tipo: Journal article |
Palavras-chave: Adult bone marrow; Cord blood; Erythroid progenitor; Globins; Liquid culture. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100005 |
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| Ohtsuki,Takashi; Himeji,Mika; Fukazawa,Harumi; Tanaka,Miho; Yamamoto,Hisako; Mimura,Akio. |
| Production of baicalein, baicalin and wogonin by liquid culture of Scutellaria baicalensis cells derived from the plant root was studied. The maximum production obtained were 119 mg/L of baicalein at two week, 1372 mg/L of baicalin at eight week, and 14 mg/L of wogonin at two week. In addition, the production of baicalin was drastically increased to 1000 mg/L level at 3-week culture, and the extremely high production rate (339 mg/L•week) was obtained. In the comparison of total antioxidative activities among baicalein, baicalin and wogonin, evaluated by thiocyanate method, it was suggested that the location of hydroxyl groups both at 5- and 6-position contributed to enhancement of radical scavenging activity, and/or methoxylation at 8-position diminished... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant; Flavonoids; Liquid culture; High-yield production; Scutellaria baicalensis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200005 |
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| Muñoz,Manuel; Seemann,Peter; Jara,Gloria; Riegel,Ricardo. |
| Rhodophiala C. Presl (Amaryllidaceae) is a genus of attractive flowering geophytes native to South America. They have ornamental value, but most species are little known and have conservation problems. The objective of this study was to optimize a micropropagation process to support the use and preservation of three Chilean native species, R. montana (Phil.) Traub, R. splendens (Rengifo) Traub., and R. ananuca (Phil.) Traub. The research evaluated the feasibility of implementing liquid medium culture and assessed the influence of different tissue culture systems on the shoot production and biomass increment of small bulbs. Three experiments were carried out. The first one determined the influences of flask size and volume of media; the second compared... |
| Tipo: Journal article |
Palavras-chave: TIS; Liquid culture; Micropropagation; Acclimatization. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400014 |
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