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| Chevallier, Frédérik. |
| Cryopreservation tests were carried out for Platymonas "Batan" sp., P. suesica, Isochrysis aff galbana, Chlorella minutissima, Nannochloris occulata, Chaetoceros gracilis and C. "BA" sp. The focus of the protocol was based on the duration of incorporation and cryoprotector concentration (DMSO). Two methods were used to compare the effects of each freezing protocol for algae survival. The first method allows evaluating survival rate through orange acridine coloration and epifluorescence microscope readings. The second one requires culturing each sample and growth curbs are established through daily readings of algal solutions optical density. Freezing the samples was either done directly through liquid nitrogen freezing (-196°C) or through slow freezing,... |
| Tipo: Text |
Palavras-chave: Freezing; DMSO; Liquid nitrogen; Micro algae; Congélation; DMSO; Azote liquide; Micro algues. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/rapport-1747.pdf |
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| Brum,Francis Maria Rocho de; Randi,Aurea Maria. |
| Rumohra adiantiformis (Forst.) Ching is a fern (Dryopteridaceae) used in floral arrangements. Spores sterilized in 15% (v/v) solution of commercial sodium hypochlorite for 10 minutes and unsterilized spores were plunged in liquid nitrogen and held for 15 minutes and for 90 days. After the cryogenic treatments, spores were taken out of liquid nitrogen and rapidly thawed out in a water bath or slowly at room temperature and were cultured in Mohr's mineral solution as modified by Dyer, kept at 25 ± 2 ºC and a 16-hours photoperiod. Statistical differences were not observed in the germination of unsterilized spores immersed or not immersed in liquid nitrogen, but when the spores were previously sterilized, a severe inhibition of germination was observed in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Liquid nitrogen; Rumohra; Storage. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042006000300016 |
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| Surenciski,Mauro Rodrigo; Flachsland,Eduardo Alberto; Terada,Graciela; Mroginski,Luis Amado; Rey,Hebe Yolanda. |
| The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums (0.75 M sucrose (24 h), 0.5 M... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryoconservation; Liquid nitrogen; Calcium alginate; Activated charcoal; Sucrose. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100002 |
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| Mroginski,Luis A.; Sansberro,Pedro A.; Scocchi,Adriana M.; Luna,Claudia; Rey,Hebe Y.. |
| Tropical Ilex species have recalcitrant seeds. This work describes experiments demonstrating the feasibility of long-term conservation of Ilex brasiliensis, I. brevicuspis, I. dumosa, I. intergerrima, I. paraguariensis, I. pseudoboxus, I. taubertiana, and I. theezans through cryopreservation of zygotic rudimentary embryos at the heart developmental stage. The embryos were aseptically removed from the seeds and precultured (7 days) in the dark, at 27± 2ºC on solidified (0.8% agar) 1/4MS medium, [consisting of quarterstrength salts and vitamins of Murashige and Skoog (1962) medium] with 3% sucrose and 0.1 mg/l Zeatin. The embryos were then encapsulated in 3% calcium alginate beads and pretreated at 24 h intervals in liquid medium supplemented with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Encapsulation-dehydration; Germplasm preservation; Liquid nitrogen; Llex. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100004 |
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