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| Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Santana,José Carlos Curvelo; Tambourgi,Elias Basile. |
| The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reversed micelles; Liquid-liquid extraction; Bromelain; Pineapple; Optimization; Surface response. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021 |
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| Ribeiro,Marcela Zanella; Silva,Daniel Pereira; Vitolo,Michele; Roberto,Inês Conceição; Pessoa-Jr,Adalberto. |
| Glucose-6-phosphate dehydrogenase (G6PDH) is an important enzyme used in biochemical and medical studies and in several analytical methods that have industrial and commercial application. This work evaluated the extraction of G6PDH in aqueous two-phase system (ATPS) of poly(ethyleneglycol) (PEG)/phosphate buffer, using as enzyme source a medium prepared through commercial baker's yeast disruption. Firstly, the effects of PEG molar mass on the enzyme partition and of homogenization and rest on the system equilibrium were investigated. Afterwards, several ATPS were prepared using statistical analysis (2² factorial design). The results, including kinetic and thermodynamic parameters for the G6PDH activity, showed partial purification of this enzyme in ATPS... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aqueous two-phase systems; Glucose-6-phosphate dehydrogenase; Liquid-liquid extraction; Saccharomyces cerevisiae. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100016 |
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| Sanson,Ananda Lima; Silva,Suéllen Cristina Rennó; Martins,Matheus Coutinho Gonçalves; Giusti-Paiva,Alexandre; Maia,Patrícia Penido; Martins,Isarita. |
| A liquid-liquid extraction (LLE) combined with high-performance liquid chromatography-diode array detection method for simultaneous analysis of four chemically and structurally different antineoplastic drugs (cyclophosphamide, doxorubicin, 5-fluorouracil and ifosfamide) was developed. The assay was performed by isocratic elution, with a C18 column (5 µm, 250 x 4.6 mm) and mobile phase constituted by water pH 4.0- acetonitrile-methanol (68:19:13, v/v/v), which allowed satisfactory separation of the compounds of interest. LLE, with ethyl acetate, was used for sample clean-up with recoveries ranging from 60 to 98%. The linear ranges were from 0.5 to 100 µg mL-1, for doxorubicin and 1 to 100 µg mL-1, for the other compounds. The relative standard deviations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antineoplastic drugs/simultaneous determination in plasma; Liquid-liquid extraction; High-performance liquid chromatography/diode array detector. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200017 |
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