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Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification Biological Sciences
Prudente, Débora de Oliveira; Paiva, Renato; Nery, Fernanda Carlota; Máximo, Wesley Pires Flausino; Silva, Luciano Coutinho.
 Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study’s objective was to assess the behavior of lateral bud’s regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Botânica criopreservação de recursos genéticos vegetais Mangabeira; Recalcitrant species; Long-term conservation; Dehydration. Botânica.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620
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Physiological, biochemical, and ultrastructural aspects of Coffea arabica L. seeds under different cryopreservation protocols Ciência e Agrotecnologia
Figueiredo,Madeleine Alves de; Rosa,Sttela Dellyzete Veiga Franco da; Ricaldoni,Marcela Andreotti; Pereira,Cristiane Carvalho; Coelho,Stefânia Vilas Boas; Silva,Luciano Coutinho.
ABSTRACT Cryopreservation is a technique that may potentially conserve the germplasm of species of the Coffea genus for an indeterminate time. The aim of this study was to evaluate the physiological, biochemical and ultrastructural characteristics of cryopreserved seeds of Coffea arabica L., cultivar Catucaí amarelo IAC 62, which was subjected to different protocols regarding dehydration, precooling, cooling, rewarming and cathode water use. According to each protocol, the seeds were subjected to fast or slow drying to moisture contents of 17 or 20% (wet basis), cooled in different ways, and then immersed in liquid nitrogen for 24 hours. Different rewarming times in a water bath were also used. Physiological, biochemical and ultrastructural analyses were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cathodic water; Dehydration; Long-term conservation; Ultrafast cooling..
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100203
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