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Detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of Klebsiella pneumoniae BJM
Dalmolin,Tanise Vendruscolo; Bianchini,Bianca Vendruscolo; Rossi,Grazielle Guidolin; Ramos,Ana Carolina; Gales,Ana Cristina; Trindade,Priscila de Arruda; Campos,Marli Matiko Anraku de.
Abstract Carbapenems are considered last-line agents for the treatment of serious infections caused by Klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. We evaluated 27 isolates of K. pneumoniae resistant to carbapenems recovered from inpatients at the University Hospital of Santa Maria-RS from July 2013 to August 2014. We carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of efflux pump by broth microdilution and loss of porin by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Genetic similarity was evaluated by ERIC-PCR. High levels of resistance were verified by the minimum inhibitory concentration for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Carbapenemases; Loss of porins; Efflux pump.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300493
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