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| Sapag,Amalia; Salinas-Luypaert,Catalina; Constenla-Muñoz,Carlos. |
| BACKGROUND: Loxoscelism is the envenomation caused by the bite of Loxosceles spp. spiders. It entails severe necrotizing skin lesions, sometimes accompanied by systemic reactions and even death. There are no diagnostic means and treatment is mostly palliative. The main toxin, found in several isoforms in the venom, is sphingomyelinase D (SMD), a phospholipase that has been used to generate antibodies intended for medical applications. Nucleic acid aptamers are a promising alternative to antibodies. Aptamers may be isolated from a combinatorial mixture of oligonucleotides by iterative selection of those that bind to the target. In this work, two Loxosceles laeta SMD isoforms, Ll1 and Ll2, were produced in bacteria and used as targets with the aim of... |
| Tipo: Journal article |
Palavras-chave: Antivenom; Loxosceles laeta; RNA aptamers; SELEX; SMD-Ll1; SMD-Ll2; Sphingomyelinase D. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100002 |
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| Arán-Sekul,Tomás; Rojas,José M.; Subiabre,Mario; Cruz,Victoria; Cortés,William; Osorio,Luis; González,Jorge; Araya,Jorge E.; Catalán,Alejandro. |
| Abstract Background Loxoscelism is a severe human envenomation caused by Loxosceles spider venom. To the best of our knowledge, no study has evaluated the presence of antibodies against Loxosceles venom in loxoscelism patients without treatment with antivenom immunotherapy. We perform a comparative analysis for the presence of antibodies capable of recognizing Loxosceles venom in a group of patients diagnosed with loxoscelism and in a group of people without loxoscelism. Methods The detection of L. laeta venom, Sicarius venom and recombinant phospholipases D from Loxosceles (PLDs) in sera from people with loxoscelism (Group 1) and from healthy people with no history of loxoscelism (Group 2) was evaluated using immuno-dot blot, indirect ELISA, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Loxosceles laeta; Heterophilic antibodies; Natural antibodies; Sicarius. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100311 |
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