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A mathematical framework for group analysis of von Willebrand factor multimeric composition following luminography BJMBR
Lopes,A.A.; Soares,R.P.S.; Maeda,N.Y..
The objective of the present study was to establish a method for quantitative analysis of von Willebrand factor (vWF) multimeric composition using a mathematical framework based on curve fitting. Plasma vWF multimers from 15 healthy subjects and 13 patients with advanced pulmonary vascular disease were analyzed by Western immunoblotting followed by luminography. Quantitative analysis of luminographs was carried out by calculating the relative densities of low, intermediate and high molecular weight fractions using laser densitometry. For each densitometric peak (representing a given fraction of vWF multimers) a mean area value was obtained using data from all group subjects (patients and normal individuals) and plotted against the distance between the peak...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Von Willebrand factor; Luminography; Densitometry; Curve fitting; Pulmonary hypertension.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100002
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