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| López Garrido, Serafín Jacobo. |
| El objetivo del presente estudio fue obtener un cultivo puro de la bacteria ruminal Streptococcus bovis a partir de líquido ruminal de ovinos alimentados con una dieta alta en sorgo (70 % en base seca). Además, se analizó su actividad fermentativa (producción de AGV y lactato) y el proceso de liofilizado como método de conservación, y se realizaron ensayos para expresar su bacteriófago. Para el aislamiento se usó un medio comercial para Streptococcus spp. (Merck) preparado bajo anaerobiosis. Para la identificación se usó el sistema API 20 Strep, reacción catalasa, morfología celular y tinción de Gram. Con la prueba API 20 Strep se comprobó que la bacteria aislada pertenece a la especie S. bovis. Esta bacteria tiene forma esférica a ovoide, de 0.8... |
| Tipo: Tesis |
Palavras-chave: Rumen; Streptococcus bovis; Aislamiento; Iiofilización; Bacteriófago; Doctorado; Ganadería; Isolation; Lyophilization; Bacteriophage. |
| Ano: 2008 |
URL: http://hdl.handle.net/10521/1402 |
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| Sardenne, Fany; Bodin, Nathalie; Metral, Luisa; Crottier, Anais; Le Grand, Fabienne; Bideau, Antoine; Brisset, Blandine; Bourjea, Jerome; Saraux, Claire; Bonhommeau, Sylvain; Kerzerho, Vincent; Bernard, Serge; Rouyer, Tristan. |
| Various protocols are currently used to study marine lipids, but there is a growing interest in working on dry samples that are easier to transport. However, reference protocols are still lacking for dry samples. In order to make recommendations on this use, lipid classes and fatty acids (FA) obtained from six analytical protocols using two different tissue states (dry vs wet) and three extraction methods (automat vs manual potter vs leaving the solvent to work on tissue) were compared. Three dry storage modes of tissue (freezer vs gas nitrogen vs dry room) during one and three months were also compared. These comparisons were made on seven marine species with different lipid profiles, including fishes, crustaceans and mollusks. Lipid classes and FA... |
| Tipo: Text |
Palavras-chave: Degradation; Freeze-drying; Lyophilization; Lipid class; Marine animal; Protocol. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00467/57898/60189.pdf |
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| Godínez, S.; Calderón, M.. |
| En este trabajo se utilizaron las especies Penicillium camemberti, Penicillium roqueforti, Aspergillus oryzae, Aspergillus niger y Mucor pusillus conservadas durante ocho años por los métodos de suelo, gel de sílice y liofilización, durante siete años por el método de suelo tradicional y modificado y por seis años en agua destilada, con el objetivo de determinar la influencia de estas técnicas de preservación sobre las características de dichas cepas al cabo de un largo período de tiempo. Para ello se realizaron determinaciones de viabilidad, características macromorfológicas y presencia de contaminantes bacterianos. Las mayores retenciones de viabilidad se obtuvieron en los métodos de gel de sílice, suelo modificado y liofilización. Se observaron... |
| Tipo: Journal Contribution |
Palavras-chave: Conservation; Soils; Lyophilization. |
| Ano: 2008 |
URL: http://hdl.handle.net/1834/4895 |
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| Rodrigues,Leticia M.; Januário,Jaqueline G. B.; Santos,Suelen S. dos; Bergamasco,Rita; Madrona,Grasiele S.. |
| ABSTRACT This study aimed to produce microcapsules using ‘jabuticaba’ byproduct through lyophilization, and evaluate the stability of the powder, intended for use in gelatin. Three formulations were prepared, including the pure lyophilized extract (E), capsule with maltodextrin (CapM), and capsule with maltodextrin and xanthan gum (CapMG). The storage stability was evaluated at 4 and 25 ºC, and in the presence and absence of light, regarding color parameters, phenolic compounds and monomeric anthocyanins. The microencapsulated powders were applied in gelatin and analyzed for sensorial acceptance. Following an investigation of the storage stability of the three powders at 4 °C for 60 days, the sample that presented the best result was then analyzed at 25 ºC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myrtaceae; Bioactive compounds; Lyophilization; Maltodextrin; Xanthan gum. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662018000600424 |
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| Olmos-Zúãiga,J.R.; Jasso-Victoria,R.; Díaz-Martínez,N.E.; Gaxiola-Gaxiola,M.O.; Sotres-Vega,A.; Heras-Romero,Y.; Baltazares-Lipp,M.; Baltazares-Lipp,M.E.; Santillán-Doherty,P.; Hernández-Jiménez,C.. |
| Various methods are available for preservation of vascular grafts for pulmonary artery (PA) replacement. Lyophilization and cryopreservation reduce antigenicity and prevent thrombosis and calcification in vascular grafts, so both methods can be used to obtain vascular bioprostheses. We evaluated the hemodynamic, gasometric, imaging, and macroscopic and microscopic findings produced by PA reconstruction with lyophilized (LyoPA) grafts and cryopreserved (CryoPA) grafts in dogs. Eighteen healthy crossbred adult dogs of both sexes weighing between 18 and 20 kg were used and divided into three groups of six: group I, PA section and reanastomosis; group II, PA resection and reconstruction with LyoPA allograft; group III, PA resection and reconstruction with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulmonary artery graft; Cryopreservation; Lyophilization; Blood vessel bioprosthesis; Prosthesis design; Vascular procedures. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200701 |
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| Muzzio,Cristian Rodrigo; Dini,Nicolás Gabriel; Simionato,Laura Daniela. |
| Determination of moisture content in lyophilized solids is fundamental to predict quality and stability of freeze-dried products, but conventional methods are time-consuming, invasive and destructive. The aim of this study was to develop and optimize a fast, inexpensive, noninvasive and nondestructive method for determination of moisture content in lyophilized mannitol, based on an NIR micro-spectrometer instead of a conventional NIR spectrometer. Measurements of lyophilized mannitol were performed through the bottom of rotating glass vials by means of a reflectance probe. The root mean standard error of prediction (RMSEP) and the correlation coefficient (R²pred), yielded by the pre-treatments and calibration method proposed, was 0.233% (w/w) and 0.994,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Near-infrared spectroscopy; Lyophilization; Multivariate analysis; Moisture content; Mannitol; Micro-spectrometer. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200010 |
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| Nunes,Claudinéia Ferreira; Ferreira,Juliano Lino; Fernandes,Mônique Carolina Nunes; Breves,Sâmera de Souza; Generoso,Andressa Leal; Soares,Bárbara Dantas Fontes; Dias,Mário Sérgio Carvalho; Pasqual,Moacir; Borem,Aluízio; Cançado,Geraldo Magela de Almeida. |
| Several extraction methods of genomic DNA for identification and characterization of genetic diversity in different plant species are routinely applied during molecular analysis. However, the presence of undesirable compounds such as polyphenols and polysaccharides is one of the biggest problems faced during the isolation and purification of high quality DNA in plants. Therefore, achievement of fast and accurate methods for DNA extraction is crucial in order to produce pure samples. Leaves of strawberry genotypes (Fragaria ananassa) have high contents of polysaccharides and polyphenols which increase the sample viscosity and decrease the DNA quality, interfering with the PCR performance. Thereby, in this study we evaluated the quality and amount of genomic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragaria ananassa; Genomic DNA; Polivinilpirrolidone; Lyophilization; PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800014 |
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