|
|
|
|
|
| Mondeguer, Florence; Antignac, Jean-philippe; Guitton, Yann; Monteau, Fabrice; Le Borgne, Sabrina; Hess, Philipp. |
| The bioactivity of phytoplankton toxins that accumulate in shellfish is almost always tested on animal model (mice assay). In spite of these advantages, the ability of this test to explain the nature of the bioactivity remains limited. Besides, the current sanitary control based on targeted methods of identification and quantification (LC-MS/MS) of known toxins, do not permit to detect unknown toxins. In order to cope with this need of identification of unknown toxic substances, a next approach based on global and differential metabolomic profiling was proposed. The first results obtained from extracts that have shown a positive toxicity in mice without the substances potentially responsible for these toxic effects have been measured by targeted methods,... |
| Tipo: Text |
Palavras-chave: Métabolomique; LC-HRMS; Biotoxines marines; Biomarqueur. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00077/18784/16352.pdf |
| |
|
|
| Mondeguer, Florence. |
| Metabolomics is a new approach to assess the contamination of organisms, environmental compartments or foodstuffs, and the impact marine pollution, (natural or anthropogenic), can have on them. Through the analysis of the variation of the metabolite profiles present in the cell, tissues, organs or even the whole organism it is possible to define a physiological state, a stage of development, or the level and nature of a contamination. Generally, analysis of metabolites correlates to experimental design built around a clear biological or environmental question, needs to be supported by sensitive and reproducible tools, and requires access to libraries "house" or international. Often, the non-targeted approach allows for evaluation of many hypotheses. Thus,... |
| Tipo: Text |
Palavras-chave: Biotoxines; Biomarqueurs; Métabolomique; Microalgues; Moules; Spectrométrie de masse haute résolution (HRMS); Workflow. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00358/46892/46777.pdf |
| |
|
|
|
