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	Provedor de dados BJMBR (1) Autor Brunialti,M.K.C. (1) Fernandes,M.L. (1) Mendes,M.E. (1) Salomão,R. (1) Palavra-chave Flagellin (1) IL-6 (1) LPS tolerance (1) MALP-2 (1) Phagocytosis (1) ROS (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2010 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Human monocytes tolerant to LPS retain the ability to phagocytose bacteria and generate reactive oxygen species BJMBR Fernandes,M.L.; Mendes,M.E.; Brunialti,M.K.C.; Salomão,R.. Tolerance to lipopolysaccharide (LPS) occurs when animals or cells exposed to LPS become hyporesponsive to a subsequent challenge with LPS. This mechanism is believed to be involved in the down-regulation of cellular responses observed in septic patients. The aim of this investigation was to evaluate LPS-induced monocyte tolerance of healthy volunteers using whole blood. The detection of intracellular IL-6, bacterial phagocytosis and reactive oxygen species (ROS) was determined by flow cytometry, using anti-IL-6-PE, heat-killed Staphylococcus aureus stained with propidium iodide and 2',7'-dichlorofluorescein diacetate, respectively. Monocytes were gated in whole blood by combining FSC and SSC parameters and CD14-positive staining. The exposure to... Tipo: Info:eu-repo/semantics/article Palavras-chave: IL-6; ROS; Phagocytosis; MALP-2; Flagellin; LPS tolerance. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900008 Registros recuperados: 1 Primeira ... 1 ... Última

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