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| Registros recuperados: 33 | |
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| MELO NETO,BERNARDO; LEITÃO,JOSEANA M.S.R.; OLIVEIRA,LUCIANO G.C.; SANTOS,SÉRGIO E.M.; CARNEIRO,SABRINA M.P.; RODRIGUES,KLINGER A.F.; CHAVES,MARIANA H.; ARCANJO,DANIEL D.R.; CARVALHO,FERNANDO A.A.. |
| ABSTRACT Zanthoxylum rhoifolium Lam. (Rutaceae) has been traditionally used in the treatment of microbial infections and parasitic diseases. In the present study, the antileishmanial effect induced by the ethanol extract of stem barks from Z. rhoifolium (ZR-EEtOH) and its n-hexane fraction (ZR-FHEX) on infection and infectivity of murine macrophages by promastigote forms of Leishmania amazonensis were investigated. In different set of experiments, macrophages or promastigotes were pretreated with ZR-EEtOH or ZR-FHEX at non-lethal concentrations for 24 hours, and then macrophages were submitted to infection by promastigotes. Moreover, their effects on activation of macrophages, as well as on the DNA content, size and number of promastigotes by flow... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Macrophages; Nitric oxide; Promastigote; Phagocytic activity; Zanthoxylum. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501851 |
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| Espinoza,Rodrigo A.; Silva‑Valenzuela,Cecilia A.; Amaya,Fernando A.; Urrutia,Ítalo M.; Contreras,Inés; Santiviago,Carlos A.. |
| Abstract Background Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI-8 at the same genomic location. Because the interaction with macrophages is a critical step in Salmonella pathogenicity, in this study we investigated the role played by SPI-13 and SPI-8 in the interaction of S. Enteritidis and S. Typhi with cultured murine (RAW264.7) and human (THP-1) macrophages. Results Our results showed that SPI-13 was required for internalization of S. Enteritidis in murine but not human macrophages. On the other hand, SPI-8 was not required for the interaction of S. Typhi... |
| Tipo: Journal article |
Palavras-chave: Salmonella; Enteritidis; Typhi; SPI-13; SPI-8; Macrophages; RAW264.7; THP-1. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100402 |
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| Galvão-Lima,Leonardo J.; Espíndola,Milena S.; Soares,Luana S.; Zambuzi,Fabiana A.; Cacemiro,Maira; Fontanari,Caroline; Bollela,Valdes R.; Frantz,Fabiani G.. |
| Abstract Objectives: Three decades after HIV recognition and its association with AIDS development, many advances have emerged – especially related to prevention and treatment. Undoubtedly, the development of Highly Active Antiretroviral Therapy (HAART) dramatically changed the future of the syndrome that we know today. In the present study, we evaluate the impact of Highly Active Antiretroviral Therapy on macrophage function and its relevance to HIV pathogenesis. Methods: PBMCs were isolated from blood samples and monocytes (CD14+ cells) were purified. Monocyte-Derived Macrophages (MDMs) were activated on classical (MGM-CSF+IFN-γ) or alternative (MIL-4+IL13) patterns using human recombinant cytokines for six days. After this period, Monocyte-Derived... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Innate immune response; HAART; Macrophages. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100042 |
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| Jhamb,Sarbjit Singh; Goyal,Amit; Singh,Prati Pal. |
| BACTEC 460 has now been phased out, so the search for an alternative is imperative. We have determined the activity of standard anti-tuberculosis drugs against intramacrophage Mycobacterium tuberculosis, in vitro, by using BACTEC 460 and MGIT 960 methods. The minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol and streptomycin against intracellular M. tuberculosis H37Rv were found to be 0.2, 0.8, 8.0, and 5.0 µg/mL, respectively, by both methods. These results show a significant (p < 0.001) concordance between minimum inhibitory concentrations obtained by these two different methods. MGIT 960 system uses a robust florescence quenching-based oxygen sensor, requires no radioisotope, is safe, and relatively easy to operate. Apparently,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: BACTEC 460; Macrophages; MGIT 960; Mycobacterium tuberculosis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300336 |
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| Herzog-Soares,Joanna D'Arc A; Freire,Ronald Bastos. |
| Macrophages exposed to 10 mug/mL citrinin (CTR) or 0.01 mug CTR mixed with 0.04 mug aflatoxin B1 (AFB1) for a period of 2 h at 37ºC, were infected with 10(6) Toxoplasma gondii tachyzoites/muL. The parasites were treated with mycotoxins (2 h at 37ºC) before being added to the macrophage culture. The number of tachyzoites was quantified 2, 24, 48, 72 and 96 h after infection. During the first 2 hours, 59% infectivity was observed in the control. After exposure to CTR or the mixture of toxins (CTR-AFB1), macrophages were infected with 77.5% and 75% of the inoculated tachyzoites, respectively. Similarly, 72.3% of the cells were infected when cultured together with previously treated parasites. The treatment with CTR-AFB1 gave rise to 2.9 times more tachyzoites... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Imunossuspression; Macrophages; Citrinin; Toxoplasma gondii. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100007 |
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| Neumann,E.; Oliveira,M.A.P.; Cabral,C.M.; Moura,L.N.; Nicoli,J.R.; Vieira,E.C.; Cara,D.C.; Podoprigora,G.I.; Vieira,L.Q.. |
| Probiotics are formulations containing live microorganisms or microbial stimulants that have some beneficial influence on the maintenance of a balanced intestinal microbiota and on the resistance to infections. The search for probiotics to be used in prevention or treatment of enteric infections, as an alternative to antibiotic therapy, has gained significant impulse in the last few years. Several studies have demonstrated the beneficial effects of lactic acid bacteria in controlling infection by intestinal pathogens and in boosting the host's nonspecific immune response. Here, we studied the use of Lactobacillus acidophilus UFV-H2b20, a lactic acid bacterium isolated from a human newborn from Viçosa, Minas Gerais, Brazil, as a probiotic. A suspension... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactobacillus; Macrophages; Kupffer cells; Probiotics. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200009 |
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| Reis,D.S.; Souza,M.A.; Mineo,J.R.; Espindola,F.S.. |
| Actin-based motor protein requirements and nitric oxide (NO) production are important features of macrophage activity during phagocytosis or microbicidal processes. Different classes of myosins contribute directly or indirectly to phagocytosis by providing mechanical force for phagosome closure or organelle movement. Recent data have shown the presence of myosins IC, II, V and IXb in phagosomes of bone marrow-derived murine macrophages. In our investigation we demonstrated the presence of different classes of myosins in J774 macrophages. We also analyzed the effect of gamma interferon (IFN-gamma), with or without calcium ionophore or cytochalasin B, on myosins as well as on inducible nitric oxide synthase (iNOS) expression and NO production. Myosins IC,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Myosins; Macrophages; IFN-gamma; INOS. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200009 |
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| Curi,R.; Newsholme,P.; Pithon-Curi,T.C.; Pires-de-Melo,M.; Garcia,C.; Homem-de-Bittencourt Jr.,P.I.; Guimarães,A.R.P.. |
| Eric Newsholme's laboratory was the first to show glutamine utilization by lymphocytes and macrophages. Recently, we have found that neutrophils also utilize glutamine. This amino acid has been shown to play a role in lymphocyte proliferation, cytokine production by lymphocytes and macrophages and phagocytosis and superoxide production by macrophages and neutrophils. Knowledge of the metabolic fate of glutamine in these cells is important for the understanding of the role and function of this amino acid in the maintenance of the proliferative, phagocytic and secretory capacities of these cells. Glutamine and glucose are poorly oxidized by these cells and might produce important precursors for DNA, RNA, protein and lipid synthesis. The high rate of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutamine; Lymphocytes; Macrophages; Neutrophils; Metabolic fate. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100002 |
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| Gazzinelli,R.T.; Talvani,A.; Camargo,M.M.; Santiago,H.C.; Oliveira,M.A.P.; Vieira,L.Q.; Martins,G.A.; Aliberti,J.C.S.; Silva,J.S.. |
| Toxoplasma gondii and Trypanosoma cruzi are intracellular parasites which, as part of their life cycle, induce a potent cell-mediated immunity (CMI) maintained by Th1 lymphocytes and IFN-<FONT FACE="Symbol">g</font>. In both cases, induction of a strong CMI is thought to protect the host against rapid parasite multiplication and consequent pathology and lethality during the acute phase of infection. However, the parasitic infection is not eliminated by the immune system and the vertebrate host serves as a parasite reservoir. In contrast, Leishmania sp, which is a slow growing parasite, appears to evade induction of CMI during early stages of infection as a strategy for surviving in a hostile environment (i.e., inside the macrophages which are... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Toxoplasma gondii; Trypanosoma cruzi; Macrophages; Cytokines; Nitric oxide. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100012 |
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| Azevedo,R.B.; Lacava,Z.G.M.; Miyasaka,C.K.; Chaves,S.B.; Curi,R.. |
| Human and animal immune functions present sex dimorphism that seems to be mainly regulated by sex hormones. In the present study, the activities of the antioxidant enzymes total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were measured in intraperitoneal resident macrophages from adult male and female rats. In addition to comparing males and females, we also examined the regulation of these enzyme activities in macrophages by sex steroids. GSH-Px activity did not differ between male and female macrophages. However, both total SOD and CAT activities were markedly higher in females than in males (83 and 180%). Removal of the gonads in both males and females (comparison between castrated groups) increased the difference in... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Antioxidant enzymes; Macrophages; Sex hormones; Estrogen; Progesterone; Castration. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500018 |
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| Grando,F.C.C.; Felício,C.A.; Twardowschy,A.; Paula,F.M.; Batista,V.G.; Fernandes,L.C.; Curi,R.; Nishiyama,A.. |
| To determine the effects of saturated and unsaturated fatty acids in phosphatidylcholine (PC) on macrophage activity, peritoneal lavage cells were cultured in the presence of phosphatidylcholine rich in saturated or unsaturated fatty acids (sat PC and unsat PC, respectively), both used at concentrations of 32 and 64 µM. The treatment of peritoneal macrophages with 64 µM unsat PC increased the production of hydrogen peroxide by 48.3% compared to control (148.3 ± 16.3 vs 100.0 ± 1.8%, N = 15), and both doses of unsat PC increased adhesion capacity by nearly 50%. Moreover, 64 µM unsat PC decreased neutral red uptake by lysosomes by 32.5% compared to the untreated group (67.5 ± 6.8 vs 100.0 ± 5.5%, N = 15), while both 32 and 64 µM unsat PC decreased the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phosphatidylcholine; Lecithin; Macrophages; Fatty acids; Reactive oxygen species; Phagocytosis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700003 |
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| Pereira,C.A.; Modolell,M.; Frey,J.R.; Lefkovits,I.. |
| Macrophages are critical for natural immunity and play a central role in specific acquired immunity. The IFN-gamma activation of macrophages derived from A/J or BALB/c mice yielded two different patterns of antiviral state in murine hepatitis virus 3 infection, which were related to a down-regulation of the main virus receptor. Using cDNA hybridization to evaluate mRNA accumulation in the cells, we were able to identify several genes that are differently up- or down-regulated by IFN-gamma in A/J (267 and 266 genes, respectively, up- and down-regulated) or BALB/c (297 and 58 genes, respectively, up- and down-regulated) mouse macrophages. Macrophages from mice with different genetic backgrounds behave differently at the molecular level and comparison of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene array; MRNA; Mouse hepatitis virus 3 (MHV3); IFN-gamma; BALB/c mouse; A/J mouse; Macrophages. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200005 |
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| Neumann,E.; Ramos,M.G.; Santos,L.M.; Rodrigues,A.C.P.; Vieira,E.C.; Afonso,L.C.C.; Nicoli,J.R.; Vieira,L.Q.. |
| Lactobacillus delbrueckii UFV-H2b20 has been shown to increase clearance of bacteria injected into the blood of germ-free mice. Moreover, it induces the production of type 1 cytokines by human peripheral mononuclear cells. The objective of the present study was to investigate the production of inflammatory cytokines [interleukin-12 (IL-12 p40), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ)] triggered in vitro by live, heat-killed or lysozyme-treated L. delbrueckii UFV-H2b20 and in vivo by a live preparation. Germ-free, L. delbrueckii-monoassociated and lipopolysaccharide (LPS)-resistant C3H/HeJ mice were used as experimental models. UFV-H2b20 induced the production of IL-12 p40 and TNF-α by peritoneal cells and IFN-γ by spleen cells from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Probiotics; Cytokines; Macrophages; IFN-γ; IL-12; Lactobacillus. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400008 |
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| Registros recuperados: 33 | |
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