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Effect of chickpea (Cicer arietinum L.) germination on the major globulin content and in vitro digestibility Ciênc. Tecnol. Aliment.
Portari,Guilherme Vanucchi; Tavano,Olga Luisa; Silva,Maraiza A. da; Neves,Valdir Augusto.
Chickpea seed germination was carried out over a period of 6 days. Little variation in the nitrogen and total globulin content was observed. The major globulin (11 S type) showed higher variation after the 4th day of germination. The elution behaviour and distribution of the isolated major globulin fraction on Sepharose CL-6B chromatography showed little modification at the end of germination. On SDS-PAGE the peak eluted from Sepharose CL-6B showed changes in protein bands between 20 and 30 kDa and above 60 kDa, indicating protein degradation during the period. Proteolytic activity was detected in the albumin fraction of the seeds, which increased up to the fourth and then decreased up to the sixth day, when isolated chickpea total globulin and casein were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chickpea germination; Protein fractions; Major globulin; Protease activity; In vitro digestibility.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400029
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