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Expression pattern of drought stress marker genes in soybean roots under two water deficit systems Genet. Mol. Biol.
Neves-Borges,Anna Cristina; Guimarães-Dias,Fábia; Cruz,Fernanda; Mesquita,Rosilene Oliveira; Nepomuceno,Alexandre Lima; Romano,Eduardo; Loureiro,Marcelo Ehlers; Grossi-de-Sá,Maria de Fátima; Alves-Ferreira,Márcio.
The study of tolerance mechanisms for drought stress in soybean is fundamental to the understanding and development of tolerant varieties. Using in silico analysis, four marker genes involved in the classical ABA-dependent and ABA-independent pathways of drought response were identified in the Glycine max genome in the present work. The expression profiles of the marker genes ERD1-like, GmaxRD20A-like, GmaxRD22-like and GmaxRD29B-like were investigated by qPCR in root samples of drought sensitive and tolerant soybean cultivars (BR 16 and Embrapa 48, respectively), submitted to water deficit conditions in hydroponic and pot-based systems. Among the four putative soybean homologs to Arabidopsis genes investigated herein, only GmaxRD29B-like was not regulated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Water deficit; Drought adaptation; Marker genes; Glycine max.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200003
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Positive, negative and marker-free strategies for transgenic plant selection Braz. J. Plant Physiol.
Aragão,Francisco José Lima; Brasileiro,Ana Cristina Miranda.
In this review, the use of the most common selection marker genes on plant transformation and the effects of their respective selective agents are discussed. These genes could be divided in two categories according their mode of action: genes for positive and negative selection. The retention of the marker gene flow through chloroplast transformation is also discussed. Further, strategies to recover marker-free transgenic plants, involving multi-auto-transformation (MAT), co-transformation, site-specific recombination and intragenomic relocation of transgenes through transposable elements are reviewed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibiotic resistance; Herbicide resistance; Marker genes; Plant transformation; Selection; Transgenic plant.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100001
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