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Tailoring antimicrobials in febrile neutropenia: using faster diagnostic and communication tools to improve treatment in the era of extensively resistant pathogens BJID
Souza,Ingvar Ludwig Augusto de; Quiles,Milene Gonçalves; Boettger,Bruno Cruz; Pignatari,Antonio Carlos Campos; Cappellano,Paola.
ABSTRACT Febrile Neutropenia represents a medical emergency and the use of appropriate antimicrobial therapy is essential for a better outcome. Although being time-consuming, conventional cultures and antimicrobial susceptibility tests remain the golden standard practices for microbiology identification. Final reports are typically available within several days. Faster diagnostic tools, such as species identification trough Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) and molecular techniques might help to shorten time to diagnostic and also guide definitive therapy in this scenario. Here we present a case in which the use of a diagnostic molecular workflow combining MALDI-TOF and real-time PCR for relevant genes codifying...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Febrile Neutropenia; Molecular Diagnosis; Mass Spectrometry; Communication.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300239
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A Method for Detection of Trace Concentrations of Underivatized Amino Acid in Hydrothermal Fluids by Ion-Pairing Reversed-Phase UPLC-ESI-QTOF-MS ArchiMer
Konn, Cecile; Magner, Jörgen; Charlou, Jean-luc; Holm, Nils G; Alsberg, Tomas.
Investigation of amino acids in hydrothermal systems is of prime importance for the understanding of geochemistry and microbiology of hydrothermal vents and plumes, for carbon and metals global cycles, for metabolism of some hydrothermal microorganisms and for the origin of life issue. Extensive theoretical and experimental work on amino acids behaviour in hydrothermal fluids has been done, conversely only few data exist on natural samples. Because each hydrothermal vent is unique, the more data we collect the better we will be able to address each of these questions. Usually amino acids in hydrothermal fluids have been measured by HPLC-FLD. The chromatographic separation was at least 26 min and up to 135 min and the required derivatization step may be...
Tipo: Text Palavras-chave: Amino Acids; UPLC; Ion-Pairing; QTOF; Mass Spectrometry; Hydrothermal Fluids.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00271/38188/36339.pdf
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