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Biological control of banana black Sigatoka disease with Trichoderma Ciência Rural
Cavero,Poholl Adan Sagratzki; Hanada,Rogério Eiji; Gasparotto,Luadir; Coelho Neto,Rosalee Albuquerque; Souza,Jorge Teodoro de.
Black Sigatoka disease caused by Mycosphaerella fijiensis is the most severe banana disease worldwide. The pathogen is in an invasive phase in Brazil and is already present in most States of the country. The potential of 29 isolates of Trichoderma spp. was studied for the control of black Sigatoka disease under field conditions. Four isolates were able to significantly reduce disease severity and were further tested in a second field experiment. Isolate 2.047 showed the best results in both field experiments and was selected for fungicide sensitivity tests and mass production. This isolate was identified as Trichoderma atroviride by sequencing fragments of the ITS region of the rDNA and tef-1α of the RNA polymerase. Trichoderma atroviride was as effective...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonists; Fungicide sensitivity; Mass production; Mycosphaerella fijiensis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000600951
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Production technology for entomopathogenic fungus using a biphasic culture system PAB
Machado,Ana Carolina Ribeiro; Monteiro,Antonio Carlos; Almeida,Aline Maria Belasco de; Martins,Maria Inez Espagnoli Geraldo.
The objective of this research was to evaluate combinations of liquid media obtained from agro-industrial residues and by-products, with solid media prepared with mixtures of grains and their derivatives, aiming to increase the production of JAB 02 and JAB 45 isolates of Lecanicillium lecanii. Sporulation, conidial viability and process yield were evaluated as well as the production costs using the JAB 45 isolate as a model system were analyzed. The production of JAB 02 was not increased using the biphasic culture. For JAB 45, some combinations provided an increase in yield, especially cheese whey with wheat bran and wheat grain, with lower production costs. Viability was not influenced by the production method, and the combinations showed no differences...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lecanicillium lecanii; Conidial viability; Liquid media; Mass production; Solid media.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001000015
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New method for rearing Spodoptera frugiperda in laboratory shows that larval cannibalism is not obligatory Rev. Bras. entomol.
Silva,Cherre Sade Bezerra Da; Parra,José Roberto Postali.
New method for rearing Spodoptera frugiperda in laboratory shows that larval cannibalism is not obligatory. Here we show, for the first time, that larvae of the fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera, Noctuidae), can be successfully reared in a cohort-based manner with virtually no cannibalism. FAW larvae were reared since the second instar to pupation in rectangular plastic containers containing 40 individuals with a surprisingly ca. 90% larval survivorship. Adult females from the cohort-based method showed fecundity similar to that already reported on literature for larvae reared individually, and fertility higher than 99%, with the advantage of combining economy of time, space and material resources. These findings suggest that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artificial diet; Cannibalistic behavior; Fall armyworm; Mass production; Rearing technique.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000300015
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