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| Razmkhah,Farnaz; Soleimani,Masoud; Ghasemi,Sorayya; Kafi-abad,Sedigheh Amini. |
| Abstract Microvesicles are able to induce the cell of origin’s phenotype in a target cell. MicroRNA-21, as an oncomir, is up-regulated in almost all cancer types such as leukemia which results in cell proliferation. In this study, we examine the ability of leukemia microvesicles to induce proliferation in hematopoietic stem progenitor cells (HSPCs) via microRNA-21 dysregulation. Herein, leukemia microvesicles were isolated from HL-60 and NB-4 cell lines by ultracentrifugation, and then their protein content was measured. Normal HSPCs were isolated from umbilical cord blood samples by a CD-34 antibody. These cells were treated with 20 and 40 μg/mL leukemia microvesicles for 5 and 10 days, respectively. Cell count, CD-34 analysis, and a microRNA-21 gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leukemia; Microvesicles; Hematopoietic stem cells; MicroRNA-21. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300465 |
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| Chen,B.; Huang,S.G.; Ju,L.; Li,M.; Nie,F.F.; Zhang,Y.; Zhang,Y.H.; Chen,X.; Gao,F.. |
| This study aims to explore the effect of microRNA-21 (miR-21) on the proliferation of human degenerated nucleus pulposus (NP) by targeting programmed cell death 4 (PDCD4) tumor suppressor. NP tissues were collected from 20 intervertebral disc degeneration (IDD) patients, and from 5 patients with traumatic spine fracture. MiR-21 expressions were tested. NP cells from IDD patients were collected and divided into blank control group, negative control group (transfected with miR-21 negative sequences), miR-21 inhibitor group (transfected with miR-21 inhibitors), miR-21 mimics group (transfected with miR-21 mimics) and PDCD4 siRNA group (transfected with PDCD4 siRNAs). Cell growth was estimated by Cell Counting Kit-8; PDCD4, MMP-2,MMP-9 mRNA expressions were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Intervertebral disc degeneration; MicroRNA-21; Human nucleus pulposus cells; Matrix metalloproteinase-2; Matrix metalloproteinase-9; Target gene. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600602 |
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