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	Provedor de dados Electron. J. Biotechnol. (2) Ciênc. Tecnol. Aliment. (1) Biological Sciences (1) Autor ALVES,Érika de Pádua (1) Busse,Nadine (1) Colognesi, Geyci de Oliveira (1) Czermak,Peter (1) KAKUNO,Amanda Sayuri Senoo (1) Lai,Daowan (1) Liu,Yang (1) Luo,Haiyu (1) MORIOKA,Luiz Rodrigo Ito (1) Morioka, Luiz Rodrigo Ito (1) Oppermann,Tobias (1) PAIÃO,Fernanda Gonzales (1) SUGUIMOTO,Hélio Hiroshi (1) Schwarz,Stefan (1) Suguimoto, Hélio Hiroshi (1) TAKIHARA,Anderson Massaro (1) VIANA,Caroline dos Santos (1) Wang,Jian (1) Xie,Rushan (1) Xu,Dan (1) Mais... Palavra-chave Microbial biotechnology (4) ATP assay (1) Bacterial pathogen (1) Beta-galactosidase (1) Bioactivities (1) Bovine respiratory disease (1) Cattle industry (1) Cell permeabilization (1) Central composite design (1) Ciência dos alimentos permeabilizing agent (1) Combination effects (1) Critical parameters (1) Enzyme activity (1) Enzyme extraction (1) Lactose hydrolysis (1) Lipopolysaccharides (1) Mannheimia haemolytica (1) Optimal conditions Biotecnologia (1) Polymyxin B (1) Process biotechnology (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Journal article (2) Ano 2019 (1) 2016 (3) País Brazil (2) Chile (2) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Permeabilization of Saccharomyces fragilis IZ 275 cells with ethanol to obtain a biocatalyst with lactose hydrolysis capacity Biological Sciences Morioka, Luiz Rodrigo Ito; Colognesi, Geyci de Oliveira; Suguimoto, Hélio Hiroshi. The permeabilization was used to transform microorganisms in cell biocatalysts with high enzymatic activity. The Saccharomyces fragilis IZ 275 yeast cells were permeabilized with ethanol, as permeabilizing agent. To optimize the permeabilization conditions were used the design of Box-Behnken 15 trials (3 central points). The independent variables and their levels were ethanol (29, 32 and 35%), temperature (15, 20 and 25°C) and time (15, 20 and 25 min). The answer (Y) function has beta-galactosidase activity (U mg-1). The optimum conditions for obtaining a high enzymatic activity were observed in 35% ethanol concentration, temperature 15ºC and 20 min. treatment time. The maximum activity of the enzyme beta-galactosidase obtained was 10.59 U mg-1. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciência dos alimentos permeabilizing agent; Beta-galactosidase; Microbial biotechnology; Optimal conditions Biotecnologia. Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/29220 Concentrated beta-galactosidase and cell permeabilization from Saccharomyces fragilis IZ 275 for beta-galactosidase activity in the hydrolysis of lactose Ciênc. Tecnol. Aliment. MORIOKA,Luiz Rodrigo Ito; VIANA,Caroline dos Santos; ALVES,Érika de Pádua; PAIÃO,Fernanda Gonzales; TAKIHARA,Anderson Massaro; KAKUNO,Amanda Sayuri Senoo; SUGUIMOTO,Hélio Hiroshi. Abstract The cheese whey shows an organic nutrient charge that can be used to obtain metabolites of interest by biotechnology of microorganisms. Thus, fermentative processes for enzyme production, in particular beta-galactosidase becomes feasible. The enzyme plays an important role in the biotech food industry to obtain milk and dairy products with low lactose content for consumption by intolerant individuals. The objective of this work was to determine the enzyme activity of the concentrated beta-galactosidase (CBG) and the permeabilized cells (PC) both obtained from Saccharomyces fragilis IZ 275. The enzyme beta-galactosidase obtained from the fermentation of Saccharomyces fragilis IZ 275 in cheese whey was used to determine the optimal conditions for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme activity; Microbial biotechnology; Cell permeabilization; Lactose hydrolysis; Enzyme extraction. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000300524 A fast and simple assay to quantify bacterial leukotoxin activity Electron. J. Biotechnol. Oppermann,Tobias; Schwarz,Stefan; Busse,Nadine; Czermak,Peter. Background: Mannheimia haemolytica is the primary bacterial pathogen in causing bovine respiratory disease with tremendous annual losses in the cattle industry. The leukotoxin from M. haemolytica is the predominant virulence factor. Several leukotoxin activity assays are available but not standardized regarding sample preparation and cell line. Furthermore, these assays suffer from a high standard error, a prolonged time consumption and often complex sample pretreatments, which is important from the bioprocess engineering point of view. Results: Within this study, an activity assay based on the continuous cell line BL3.1 combined with a commercial available adenosine triphosphate viability assay kit was established. The leukotoxin activity was found to be... Tipo: Journal article Palavras-chave: ATP assay; Bacterial pathogen; Bovine respiratory disease; Cattle industry; Critical parameters; Lipopolysaccharides; Mannheimia haemolytica; Microbial biotechnology; Polymyxin B; Quantification; Virulence. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600006 Enhancement of botrallin and TMC-264 production in liquid culture of endophytic fungus Hyalodendriella sp. Ponipodef12 after treatments with metal ions Electron. J. Biotechnol. Luo,Haiyu; Xu,Dan; Xie,Rushan; Zhang,Xuping; Wang,Jian; Xuejiao,Dong; Lai,Daowan; Zhou,Ligang; Liu,Yang. Background: Hyalodendriella sp. Ponipodef12, an endophytic fungus from a poplar hybrid, was a high producer of botrallin and TMC-264 with various bioactivities. In this study, the influences of eight metal ions (i.e.,Mn2+,Na+, Mg2+,Zn2+,Cu2+,Fe2+,Fe3+ and Al3+) on botrallin and TMC-264 production in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 were investigated. Results: Three most effective metal ions (Zn2+,Cu2+ and Mg2+) along with their optimum concentrations were screened. The optimum addition time and concentrations of Zn2+,Cu2+ and Mg2+ were further obtained respectively for improving botrallin and TMC-264 production. The combination effects of Zn2+,Cu2+ and Mg2+ on the production of botrallin and TMC-264 by employing... Tipo: Journal article Palavras-chave: Combination effects; Production; Microbial biotechnology; Process biotechnology; Bioactivities; Central composite design; Response surface methodology. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600002 Registros recuperados: 4 Primeira ... 1 ... Última

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