|
|
|
|
|
| Menegat, Francieli Bortoluzzi; Baldo, Cristiani; Celligoi, Maria Antonia Pedrine Colabone; Buzato, João Batista. |
| Asparaginase is an enzyme used in clinical treatments as a chemotherapeutic agent and in food technology to prevent acrylamide formation in fried and baked foods. Asparaginase is industrially produced by microorganisms, mainly gram-negative bacteria. Zymomonas mobilis is a Gram-negative bacterium that utilizes glucose, fructose and sucrose as carbon source and has been known for its efficiency in producing ethanol, sorbitol, levan, gluconic acid and has recently aroused interest for asparaginase production. Current assay optimizes the production of Z. mobilis asparaginase by continuous fermentation using response surface experimental design and methodology. The studied variables comprised sucrose, yeast extract and asparagine. Optimized condition obtained... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sucrose; Asparagine; Yeast extract; Enzyme; Microbial production; Factorial design. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31057 |
| |
|
|
| Preichardt, Leidi Daiana; Haubert, Louise; Sawitzki, Maristela Cortez; Bertol, Teresinha Marisa; Vicenzi, Raul; Meinhart, Adriana Dillenburg; Silva, Wladimir Padilha da; Fiorentini, Ângela Maria. |
| Staphylococcus xylosus is a microorganism that has important physiological and technological characteristics that make it suitable for use as a starter culture in fermented meat products. For the development of these products in the food industry, it is necessary to produce biomass by the multiplication of starter cultures using low-cost media. This study developed a culture medium based on sugarcane molasses (SCM) supplemented with yeast extract (YE) and soybean meal (SM) to produce S. xylosus AD1 biomass employing a Box Behnken multivariate optimization design, using the best concentrations of the constituents of the culture medium for S. xylosus AD1 growth. By combining the mathematical models by the desirability function, it was possible to establish... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agro-industrial by products; Microbial production; Starter culture.. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47487 |
| |
|
|
| Cachumba,Jorge Javier Muso; Antunes,Felipe Antonio Fernandes; Peres,Guilherme Fernando Dias; Brumano,Larissa Pereira; Santos,Júlio César Dos; Silva,Silvio Silvério Da. |
| ABSTRACT L-asparaginase (EC 3.5.1.1) is an enzyme that catalysis mainly the asparagine hydrolysis in L-aspartic acid and ammonium. This enzyme is presented in different organisms, such as microorganisms, vegetal, and some animals, including certain rodent's serum, but not unveiled in humans. It can be used as important chemotherapeutic agent for the treatment of a variety of lymphoproliferative disorders and lymphomas (particularly acute lymphoblastic leukemia (ALL) and Hodgkin's lymphoma), and has been a pivotal agent in chemotherapy protocols from around 30 years. Also, other important application is in food industry, by using the properties of this enzyme to reduce acrylamide levels in commercial fried foods, maintaining their characteristics (color,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: L-asparaginase; Microbial production; Industrial production; Pharmaceutical application; Acrylamide. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500077 |
| |
|
|
|
