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| Registros recuperados: 81 | |
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| Martínez Martínez, Susana. |
| Bremia lactucae Regel causa una de las enfermedades de mayor importancia en la lechuga a nivel mundial. Debido a que este pseudohongo presenta dos tipos de reproducción: sexual y asexual, su diversidad genética es amplia y puede dar origen a nuevas razas. Dicha situación se desconoce en México, por lo que es importante conocer este aspecto en las zonas productoras de esta hortaliza, así como también la influencia que ejercen los factores ambientales sobre la incidencia y severidad del patógeno. Por ello, fueron determinadas la incidencia y severidad de B. lactucae en 15 variedades de lechuga, además se registraron variables ambientales como la temperatura, humedad relativa, humedad de la hoja y precipitación en los municipios de Salamanca y San Miguel de... |
| Tipo: Tesis |
Palavras-chave: Bremia lactucae; Mildiu; Diversidad genética; Microsatélites; Maestría; Fitopatología; Mildew; Genetic diversity; Microsatellite. |
| Ano: 2008 |
URL: http://hdl.handle.net/10521/1675 |
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| Machado,Mariana B.B.; Alencar,Maurício M.; Pereira,Andréa P.; Oliveira,Henrique N.; Casas,Eduardo; Coutinho,Luis L.; Regitano,Luciana C.A.. |
| The objective of this work was to identify QTLs for liveweight in a candidate region of bovine chromosome 5. Half-sib families from two lines, one traditional and the other new, of Canchim beef cattle (5/8 Charolais + 3/8 Zebu) were genotyped for four microsatellite markers, including the microsatellite in the IGF-1 (insulin-like growth factor-1) promoter region. Significant differences in allele distribution between the two lines were found for three markers. Interval mapping analyses in this region indicated the presence of a QTL controlling birth weight (p < 0.05) and of a QTL influencing breeding value for yearling weight (p < 0.01) in the newer line of the breed. The previously identified interaction between the IGF-1 genotype and genetic group... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: QTL; Growth traits; Microsatellite; Bovine. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300008 |
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| Castilho,Camila S.; Marins-Sá,Luiz G.; Benedet,Rodrigo C.; Freitas,Thales R.O.. |
| The Brazilian Atlantic Rain Forest, one of the most endangered ecosystems worldwide, is also among the most important hotspots as regards biodiversity. Through intensive logging, the initial area has been reduced to around 12% of its original size. In this study we investigated the genetic variability and structure of the mountain lion, Puma concolor. Using 18 microsatellite loci we analyzed evidence of allele dropout, null alleles and stuttering, calculated the number of allele/locus, PIC, observed and expected heterozygosity, linkage disequilibrium, Hardy-Weinberg equilibrium, F IS, effective population size and genetic structure (MICROCHECKER, CERVUS, GENEPOP, FSTAT, ARLEQUIN, ONESAMP, LDNe, PCAGEN, GENECLASS software),we also determine whether there... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Araucaria Forest; Atlantic Rain Forest; Conservation; Genetic diversity; Microsatellite. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100009 |
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| Fernández,María E.; Goszczynski,Daniel E.; Lirón,Juan P.; Villegas-Castagnasso,Egle E.; Carino,Mónica H.; Ripoli,María V.; Rogberg-Muñoz,Andrés; Posik,Diego M.; Peral-García,Pilar; Giovambattista,Guillermo. |
| During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires a marker set with enough power to identify individuals and their parents. Genetic diagnostics generally include the analysis of related animals. In this work, the degree of information provided by SNPs for a consanguineous herd of cattle was compared with that provided by STRs. Thirty-six closely related Angus cattle were genotyped for 18 STRs and 116 SNPs. Cumulative SNPs exclusion power values (Q) for paternity and sample matching... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellite; Single nucleotide polymorphism; Exclusion probability; Genetic identification; Bovine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200008 |
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| Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. |
| Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005 |
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| Angelici,Carla M.L.C.D.; Hoshino,Andrea Akemi; Nóbile,Paula Macedo; Palmieri,Dario Abel; Valls,José F. Montenegro; Gimenes,Marcos A.; Lopes,Catalina Romero. |
| The genus Arachis (Fabaceae) native to South America, contains 80 species divided into nine sections, three of which contain species of special economic importance such as the cultivated peanut (Arachis hypogaea), belonging to the section Arachis, and some perennial forage species from sections Caulorrhizae and Rhizomatosae. We used microsatellite markers to assay genetic variability among 77 accessions of four species from section Rhizomatosae, the diploid Arachis burkartii (2n = 2x = 20) and the tetraploid Arachis glabrata, Arachis pseudovillosa and Arachis nitida (2n = 4x = 40). A total of 249 alleles were found in the fifteen loci analyzed and a high degree of intra and interspecific polymorphism was detected. The lowest intraspecific variation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arachis; Genetic diversity; Microsatellite; Peanut; Rhizomatosae. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100016 |
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| Arruda,Mauricio P.; Costa,William P.; Recco-Pimentel,Shirlei M.. |
| Abstract The Morato's Digger Toad, Proceratophrys moratoi, is a critically endangered toad species with a marked population decline in southern Brazilian Cerrado. Despite this, new populations are being discovered, primarily in the northern part of the distribution range, which raises a number of questions with regard to the conservation status of the species. The present study analyzed the genetic diversity of the species based on microsatellite markers. Our findings permitted the identification of two distinct management units. We found profound genetic structuring between the southern populations, on the left margin of the Tietê River, and all other populations. A marked reduction was observed in the contemporary gene flow among the central populations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proceratophrys moratoi; Microsatellite; Population structure; Management unit; Conservation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300502 |
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| Barbosa,Ana Angélica Leal; Sousa,Sandra Mara Bispo; Abé-Sandes,Kiyoko; Alonso,Carlos Alberto; Schneider,Vicente; Costa,Denise C.C.; Cavalli,Iglenir João; Azevêdo,Eliane Elisa Souza. |
| The African descent population of the Bananal community in the Brazilian state of Bahia (BA) was characterized as a genetic isolate and analyzed for some short tandem repeat (STR) microsatellite autosomic polymorphic loci (CSF1PO, TH01, TPOX, F13A1, FESFPS and vWA). These genetic variants were further compared to data obtained from an urban sample from the town of Jequié (BA) regarding demographic and anthropogenetic aspects. The Bananal sample comprised 32 unrelated individuals whereas Jequié was represented by 76 individuals. The Bananal Negroid Phenotypic Index (NPI) was 0.98 and the Negroid Cultural Index (NCI) 0.24. Consanguineous marriages occurred at a frequency of 34.61% and the F value was 0.0126. All six loci studied were in Hardy-Weinberg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellite; African-descendant isolate. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100004 |
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| Burgos-Paz,William; Cerón-Muñoz,Mario; Solarte-Portilla,Carlos. |
| The aim was to establish the genetic diversity and population structure of three guinea pig lines, from seven production zones located in Nariño, southwest Colombia. A total of 384 individuals were genotyped with six microsatellite markers. The measurement of intrapopulation diversity revealed allelic richness ranging from 3.0 to 6.56, and observed heterozygosity (Ho) from 0.33 to 0.60, with a deficit in heterozygous individuals. Although statistically significant (p < 0.05), genetic differentiation between population pairs was found to be low. Genetic distance, as well as clustering of guinea-pig lines and populations, coincided with the historical and geographical distribution of the populations. Likewise, high genetic identity between improved and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food security; Microsatellite; Population structure. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400027 |
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| Abreu,Marcia Matos de; Pereira,Luiz Henrique Garcia; Vila,Vilma Barretto; Foresti,Fausto; Oliveira,Claudio. |
| Catfishes of the genus Pseudoplatystoma are very important species due to both their high commercial value and their ecological role as voracious predators. They undertake lengthy migratory movements during their life-cycle, this including reproductive migration which occurs from October to December in the rainy season. In the present study, seven microsatellite loci were analyzed to access genetic variability in two samples of P. reticulatum from the Upper Paraguay Basin. The loci were highly polymorphic (mean = 7.28). According to all analysis, the two samples of P. reticulatum revealed pronounced genetic differentiation. Fst value was 0.2290, Rst value 0.1067 and AMOVA 22.90% (Fst) and 10.67% (Rst), all being highly significant (p < 0.001). The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish; Population genetics; Microsatellite; Homing; Population structure. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400032 |
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| Kashiani,Pedram; Saleh,Ghizan; Panandam,Jothi Malar; Abdullah,Nur Ashikin Psyquay; Selamat,Ahmad. |
| A study of genetic variation among 10 pairs of chromosomes extracted from 13 tropical sweet corn inbred lines, using 99 microsatellite markers, revealed a wide range of genetic diversity. Allelic richness and the number of effective alleles per chromosome ranged from 2.78 to 4.33 and 1.96 to 3.47, respectively, with respective mean values of 3.62 and 2.73. According to the Shannon's information index (I) and Nei's gene diversity coefficient (Nei), Chromosome 10 was the most informative chromosome (I = 1.311 and Nei = 0.703), while Chromosome 2 possessed the least (I = 0.762 and Nei = 0.456). Based on linkage disequilibrium (LD) measurements for loci less than 50 cM apart on the same chromosome, all loci on Chromosomes 1, 6 and 7 were in equilibrium. Even... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellite; Inbred line; Chromosome; Genetic variation; Linkage disequilibrium. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400012 |
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| Presti,Flavia T.; Oliveira-Marques,Adriana R.; Caparroz,Renato; Biondo,Cibele; Miyaki,Cristina Y.. |
| Cross-amplification was tested and variability in microsatellite primers (designed for Neotropical parrots) compared, in five macaw species, viz., three endangered blue macaws (Cyanopsitta spixii [extinct in the wild], Anodorhynchus leari [endangered] and Anodorhynchus hyacinthinus [vulnerable]), and two unthreatened red macaws (Ara chloropterus and Ara macao). Among the primers tested, 84.6% successfully amplified products in C. spixii, 83.3% in A. leari, 76.4% in A. hyacinthinus, 78.6% in A. chloropterus and 71.4% in A. macao. The mean expected heterozygosity estimated for each species, and based on loci analyzed in all the five, ranged from 0.33 (A. hyacinthinus) to 0.85 (A. macao). As expected, the results revealed lower levels of genetic variability... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Cross-amplification; Macaw; Microsatellite; Psittaciformes. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200030 |
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| González-Pérez,Miguel A.; Newton,Craig; Sosa,Pedro A.; Rivero,Elizabeth; González-González,Edna A.. |
| Six novel polymorphic microsatellite markers were isolated from enriched libraries in Myrica faya Ait., recently renamed Morella faya , (fayatree, firetree, or firebush) in order to examine the genetic diversity in natural populations. Also, test cross-specific amplification and genetic diversity in Myrica rivas-martinezii, which is endemic on the Canary islands. Microsatellite loci were screened in 225 individuals of both species from different islands of the Canarian archipelago. All markers were successfully amplified from both Myrica species, with an average number of 6.5 and 9.3 alleles per locus in M. rivas-martinezii and M. faya , respectively. There was no evidence for linkage disequilibrium between loci, and the probability of null... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myrica rivas-martinezii; Myrica faya; Microsatellite; Canary Islands; Genetic diversity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100017 |
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| Lopes,Denilce Meneses; Campos,Lúcio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia. |
| Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melipona; Stingless bees; Microsatellite; Transferability. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200030 |
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| Registros recuperados: 81 | |
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