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| Registros recuperados: 149 | |
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| Yuan,Mei; Gong,Limin; Meng,Ronghua; Li,Shuangling; Dang,Phat; Guo,Baozhu; He,Guohao. |
| Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was... |
| Tipo: Journal article |
Palavras-chave: Cultivated peanut; Microsatellites; Polymorphism; Simple sequence repeat. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600005 |
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| Scali,Monica; Vignani,Rita; Bigliazzi,Jacopo; Paolucci,Elisa; Bernini,Andrea; Spiga,Ottavia; Niccolai,Neri; Cresti,Mauro. |
| Background: Cinta Senese (CS) is an autochthonous Tuscan breed, which risked extinction since the 60s. Results: Monitoring the genetic variability of the actual population by use of DNA molecular markers is essential to address a correct breeding policy, finalized to obtain the race preservation and its fitness in the future. 17 SSRs autosomal markers and 1 associated to the X chromosome were used to genotype 86 individuals belonging to the CS and 12 belonging to two main white races Landrace (L), Large White (LW) and crosses between LW and L and L and CS widespread in Tuscany and used in the recent past to obtain hybrids with the CS. Conclusions: A dendrogram of similarity measures the relative genetic distance between individuals in the population. Data... |
| Tipo: Journal article |
Palavras-chave: Cinta Senese; Genetic diversity; Genotyping; Microsatellites; Sus scrofa. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200001 |
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| Njung'e,Vincent; Deshpande,Santosh; Siambi,Moses; Jones,Richard; Silim,Said; De Villiers,Santie. |
| Background: Pigeonpea (Cajanus cajan (L.) Millsp.) is a drought tolerant legume of the Fabaceae family and the only cultivated species in the genus Cajanus. It is mainly cultivated in the semi-arid tropics of Asia and Oceania, Africa and America. In Malawi, it is grown as a source of food and income and for soil improvement in intercropping systems. However, varietal contamination due to natural outcrossing causes significant quality reduction and yield losses. In this study, 48 polymorphic SSR markers were used to assess the diversity among all pigeonpea varieties cultivated in Malawi to determine if a genetic fingerprint could be identified to distinguish the popular varieties. Results: A total of 212 alleles were observed with an average of 5.58 alleles... |
| Tipo: Journal article |
Palavras-chave: Genetic variation; Genotyping; Microsatellites; Seed purity. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300008 |
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| Dejean,Cristina B.; Crouau-Roy,Brigitte; Goicoechea,Alicia S.; Avena,Sergio A.; Carnese,Francisco R.. |
| The allelic variability of four dinucleotide microsatellites located in the HLA region (MOGc, D6S265, MIB, and TNFa) was analyzed in 67 individuals representing three Amerindian populations of the Argentine Gran Chaco: Toba, Wichi and Chorote. Genomic DNA was prepared from peripheral blood and DNA was extracted using the standard phenol-chloroform procedure. Alleles were identified by PCR, using an end-labelled reverse oligonucleotide primer (fluorescent 6 - Fam labeling). Despite the low number of samples studied, a high level of gene diversity was observed in each population and for each locus. Moreover, the mean number of alleles was 7.7, 5.3, 10.0, and 7.0 at loci MOGc, D6S265, MIB and TNFa, respectively. Differentiation tests between pairs of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aboriginal Argentinians; Microsatellites; Intra- and inter-populational variation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400004 |
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| Mäder,Geraldo; Castro,Luana; Bonatto,Sandro Luis; Freitas,Loreta Brandão de. |
| Abstract Non-indigenous plants exhibit different attributes that make them aggressive competitors with indigenous plants and serious threats to biodiversity.Senecio madagascariensis (fireweed, Asteraceae), a native from southern Africa, is a strong competitor in agricultural activities and has toxic alkaloids that may result in high cattle mortality. In Brazil, this weed was collected for the first time in 1995 and has since spread quickly throughout the Pampas region. To better understand the invasion of the fireweed in South America, we used a genetic characterization with internal transcribed spacer (ITS) and microsatellite markers. Based on the ITS data, the southern Brazil populations of S. madagascariensis shared genetic homology with samples taken... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; ITS; Pampas; Weed; Spread. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100135 |
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| Caparroz,Renato; Mantellatto,Aline M.B.; Bertioli,David J.; Figueiredo,Marina G.; Duarte,José Maurício B.. |
| The complete mitochondrial genome of the brown brocket deer Mazama gouazoubira and a set of polymorphic microsatellite markers were identified by 454-pyrosequencing. De novo genome assembly recovered 98% of the mitochondrial genome with a mean coverage of 9-fold. The mitogenome consisted of 16,356 base pairs that included 13 protein-coding genes, two ribosomal subunit genes, 22 transfer RNAs and the control region, as found in other deer. The genetic divergence between the mitogenome described here and a previously published report was ∼0.5%, with the control region and ND5 gene showing the highest intraspecific variation. Seven polymorphic loci were characterized using 15 unrelated individuals; there was moderate genetic variation across most loci (mean... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Microsatellites; Mitogenome; Population genetics; 454-pyrosequencing. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300338 |
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| Oliveira,Eder Jorge; Pádua,Juliano Gomes; Zucchi,Maria Imaculada; Vencovsky,Roland; Vieira,Maria Lúcia Carneiro. |
| Microsatellites, or simple sequence repeats (SSRs), have been the most widely applied class of molecular markers used in genetic studies, with applications in many fields of genetics including genetic conservation, population genetics, molecular breeding, and paternity testing. This range of applications is due to the fact that microsatellite markers are co-dominant and multi-allelic, are highly reproducible, have high-resolution and are based on the polymerase chain reaction (PCR). When first introduced, the development of microsatellite markers was expensive but now new and efficient methods of repetitive sequence isolation have been reported, which have led to reduced costs and microsatellite-technology has been increasingly applied to several species,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; Molecular genetics; Genetic structure of populations. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200018 |
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| John,Ana Laura de Wallau; Mäder,Geraldo; Fregonezi,Jeferson N.; Freitas,Loreta B.. |
| Abstract Calibrachoa is a South-American genus comprising 27 species, several considered endemic or rare; few were subjects in genetic studies. We attempted to generate new data about the phylogenetically related and rare species C. eglandulata, C. sendtneriana, C. serrulata, and C. spathulata concerning their genetic diversity and population structure, which, coupled with their known restricted distribution, could help access their conservation status and contribute to the study of the Brazilian biodiversity. We sequenced 88 individuals for plastid intergenic spacers and genotyped 186 individuals for five microsatellite loci. Compared among each other, C. sendtneriana and C. serrulata presented the highest values of genetic diversity [π% (sd) = 0.23... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plastid DNA; Microsatellites; Threatened species; Grasslands; Herbaceous species. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100108 |
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| Dorji,Nedup; Duangjinda,Monchai; Phasuk,Yupin. |
| The genetic diversity of Bhutanese chickens needs to be understood in order to develop a suitable conservation strategy for these birds in Bhutan. In this, work, we used microsatellite markers to examine the genetic diversity of Bhutanese chickens. Four Bhutanese chicken varieties (Black plumage, Frizzle, Naked neck and Red Junglefowllike, corresponding to Yuebjha Narp, Phulom, Khuilay and Seim, respectively), two subspecies of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), two varieties of Thai native chickens (Pradhu Hang Dam and Chee; Gallus gallus domesticus) representing the Southeast Asian domestic chicken, and two commercial lines (Broiler and Single Comb White Leghorn) were genotyped with 18 microsatellites that included 16... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation genetics; Genetic comparisons; Genetic variability; Microsatellites. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400010 |
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| Registros recuperados: 149 | |
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