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Phenotypic and genotypic characterization and compatibility among genotypes to select elite clones of cupuassu Acta Amazonica
ALVES,Rafael Moysés; SILVA,Carlos Rogério de Sousa; ALBUQUERQUE,Paulo Sérgio Bevilaqua de; SANTOS,Vinicius Silva dos.
ABSTRACT The expansion of the genetic base of cultivated materials is an ongoing activity of the cupuassu (Theobroma grandiflorum) breeding program. However, the parents involved need to be genotypically and phenotypically characterized to ensure compatibility of crossings, as well as to assist in the selection of more promising individuals for hybridization. This study aimed to identify and select T. grandiflorum clones that are compatible and genetically divergent using tools such as the estimates of genotypic, phenotypic, and combined distances, as well as the compatibility rates among clones. The genetic distance analysis of the clones was performed with 14 heterologous microsatellite primers of cocoa (Theobroma cacao) that amplify the DNA of cupuassu....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Tropical fruit; Diversity; Plant breeding; Theobroma grandiflorum.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000300175
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Genetic structure of Dicksonia sellowiana Hook. (Dicksoniaceae) reveals clinal distribution along the latitudinal gradient of the Atlantic Forest Acta Botanica
Fagundes,Bruna Saviatto; Poersch,Maria Augusta; Santos,Jaqueline dos; Gaglioti,André Luiz; Labiak,Paulo Henrique; Muschner,Valeria Cunha.
ABSTRACT Dicksonia sellowiana is the only species of the genus occurring in Brazil. Its distribution is restricted to humid areas of the Atlantic Forest biome. The distribution pattern of biodiversity in this biome is known to have been influenced by historical and environmental factors, although the pattern for ferns remains unknown. This study is first to describe the genetic structure of D. sellowiana along the latitudinal gradient of the Atlantic Forest biome. We use microsatellite markers to estimate genetic diversity and structure for 267 individuals representing 14 populations of D. sellowiana from the Atlantic Forest. The results (Ho, He, Fst, Fis, distance genetic) support the hypothesis of a pattern of biodiversity discontinuity. We found greater...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Neotropics; Gene flow; Genetic diversity; Microsatellites; Tree fern.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400712
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DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) Acta Botanica
Christ,Jheniffer Abeldt; Hollunder,Renan Köpp; Carvalho,Marina Santos; Ferreira,Marcia Flores da Silva; Garbin,Mário Luís; Carrijo,Tatiana Tavares.
ABSTRACT DNA fingerprinting based on SSR amplification profiles was applied to native species of Piper from the Atlantic Forest to compare the utility of this type of molecular marker with the morphological characters traditionally applied in Piper taxonomy and identification. Fifty-one SSR markers developed for four species of Piper native to Asia and Mesoamerica were applied to 16 species, together with 63 morphological characters, for species characterization. Molecular and morphological data were analysed by cluster analysis, followed by a cluster sharpness test and the construction of a heat map to visualize the association of characters with species groups. A multivariate regression tree determined the number of loci needed for species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heat map; Microsatellites; Morphology; Multivariate analyses; Taxonomy.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400511
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Genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors Agronomy
Andrade, Elisângela Knoblauch Viega de; Andrade júnior, Valter Carvalho de; Laia, Marcelo Luiz de; Fernandes, José Sebastião Cunha; Oliveira, Altino Junior Mendes; Azevedo, Alcinei Mistico.
 This study aimed to evaluate the genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors. The experiment was conducted in the Olericulture Sector at Federal University of Jequitinhonha and Mucuri Valleys (UFVJM) and evaluated 60 sweet potato genotypes. For morphological characterization, 24 descriptors were used. For molecular characterization, 11 microsatellite primers specific for sweet potatoes were used, obtaining 210 polymorphic bands. Morphological and molecular diversity was obtained by dissimilarity matrices based on the coefficient of simple matching and the Jaccard index for morphological and molecular data, respectively. From these matrices, dendrograms were built. There is a large amount of genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fitotecnia Ipomoea batatas (L.) Lam.; Morphological markers; Microsatellites; Genetic diversity; Germplasm bank. Melhoramento genético de plantas.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32847
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Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry Agronomy
Oñate, Felipe Alberto; Hasbún, Rodrigo; Mora, Freddy; Figueroa, Carlos Rodrigo.
 The Chilean strawberry [Fragaria chiloensis (L.) Mill.] is the maternal progenitor of the commercial strawberry (Fragaria ´ ananassa Duch.), which is characterized by fruits with high organoleptic quality and is well-suited to areas where drought and salinity represent a constraint on crop growth and productivity. We examined the patterns of linkage disequilibrium, genetic diversity and population structure among 54 accessions of F. chiloensis to understand the genetic basis of this species. We used a core microsatellite marker set (n = 95) from a consensus linkage map of strawberry. A transferability rate of 82.1% (78/95) was found, and 38 markers were selected for this study. The SSR primers produced a total of 259 alleles, which varied between 112 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Microsatellites; Chilean strawberry; Marker transferability..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/34966
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Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry Agronomy
Oñate, Felipe Alberto; Hasbún, Rodrigo; Mora, Freddy; Figueroa, Carlos Rodrigo.
 The Chilean strawberry [Fragaria chiloensis (L.) Mill.] is the maternal progenitor of the commercial strawberry (Fragaria ´ ananassa Duch.), which is characterized by fruits with high organoleptic quality and is well-suited to areas where drought and salinity represent a constraint on crop growth and productivity. We examined the patterns of linkage disequilibrium, genetic diversity and population structure among 54 accessions of F. chiloensis to understand the genetic basis of this species. We used a core microsatellite marker set (n = 95) from a consensus linkage map of strawberry. A transferability rate of 82.1% (78/95) was found, and 38 markers were selected for this study. The SSR primers produced a total of 259 alleles, which varied between 112 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Microsatellites; Chilean strawberry; Marker transferability..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/34966
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Diversidade genética em populações-núcleo de Eucalyptus grandis - doi: 10.4025/actasciagron.v32i4.3727 Agronomy
Souza, Helenize Gabriela de; UNESP; Doria, Karolina Marie Alix Benedictte Van Sebroech; UNESP; Basseto, Marco Antonio; UNESP; Rosa, Daniel Dias; UNESP-FCA, Programa de Pós-graduação em Proteção de Plantas; Furtado, Edson Luiz; UNESP; Marino, Celso Luis; UNESP - IBB, Depto de Genética.
No melhoramento genético de espécies florestais, uma população base ou indivíduos superiores pré-selecionados tem importância fundamental para a manutenção do programa. Indivíduos de melhores procedências e de ampla base genética propiciam a obtenção de ganhos de forma contínua. O objetivo deste trabalho foi avaliar a diversidade genética em duas populações-núcleo de Eucalyptus grandis. Foram avaliados 39 indivíduos, sendo 19 pertencentes à população 1 e 20, à população 2, utilizando-se 14 primers microssatélite. Os fragmentos foram identificados e analisados a partir dos programas GeneScan e Genotyper, utilizando-se um sequenciador automático ABI Prism 3100. O número de alelos encontrados para cada primer variou de cinco a 15 para a população 1 e, de 8 a...
Palavras-chave: 5.01.03.05-9 populações-núcleo; Eucalyptus; Melhoramento; Microssatélites Melhoramento genético Florestal nucleus populations; Eucalyptus; Improvement; Microsatellites.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3727
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Genetic variability in a population of Astyanax scabripinnis: recent bottleneck and the possible influence of individuals with B chromosomes Biological Sciences
Limeira, Daniel Meneguello; Santos, Mateus Henrique; Mateus, Rogério Pincela; Almeida, Mara Cristina de; Artoni, Roberto Ferreira.
Access the genetic variability of endangered and isolated populations has become an important conservation tool. Astyanax scabripinnis is a well-known fish model for genetic studies, forming very isolated populations in headwaters. Besides that, this species frequently presents supernumerary chromosomes, which elevates the interest on genetic studies. Genetic diversity of an Astyanax scabripinnis population from the Atlantic Forest (Serra da Mantiqueira region, Brazil) was assessed with microsatellite markers for the first time. Since microsatellite markers are not described for this species, we tested markers described for a related species for transferability to A. scabripinnis. Six polymorphic loci were sufficiently reliable for population genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allelic deficiency; Heterozygosity excess; Fixation index; Microsatellites; Supernumerary chromosomes.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47323
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Genetic diversity assessed by microsatellite markers in the amphicarpic species Trifolium polymorphum Poir. Anais da ABC (AABC)
CONTERATO,IONARA F.; SCHIFINO-WITTMANN,MARIA TERESA; GUERRA,DIVANILDE; BÜTTOW,MIRIAM V.; AGNOL,MIGUEL DALL’; DAHMER,NAIR.
ABSTRACT Trifolium polymorphum Poir. is an amphicarpic forage legume from southern Brazil, Uruguay, Argentina, Paraguay and Chile. Information on the genetic diversity of natural populations in natural grasslands in southern Brazil is limited. In order to increase the knowledge about this species, an analysis of the genetic diversity was carried out in 10 natural populations of T. polymorphum with the use of 20 microsatellite markers. The expected heterozygosity in T. polymorphum populations ranged from 0.40 to 0.43, with a mean of 0.42. A total of 193 alleles were detected with a mean of 9.3 alleles per locus and polymorphic information content (PIC) for these markers of 0.62 to 0.89 with a mean of 0.84.The grouping based on the Jaccard’s coefficient of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Microsatellites; Natural grasslands; Pampa biome.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401685
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Molecular marker assisted selection for increasing inbreeding in S1 populations of cassava Anais da ABC (AABC)
OLIVEIRA,PAULO HENRIQUE G.A. DE; BARBOSA,ANA CLAUDIA O.; DINIZ,RAFAEL P.; OLIVEIRA,EDER J. DE; FERREIRA,CLAUDIA F..
Abstract The objective of the present work was to use MAS in self-pollinated cassava populations for obtaining individuals with high inbreeding (f) aimed at rapid development of partial inbred lines. Three progenies (F0222, F1378 and F1662) were self-pollinated, generating a total of 233 S1 individuals. The progenies and the S1 individuals were evaluated in the seedlings and clonal evaluation trials (CETs). In the CET, plants were evaluated for the following traits: plant height (PH), root dry matter content (DMC), above ground yield (AGY), root (RY) and starch yield (SY). Twenty-seven microsatellites and five minisatellites were used to determine the level of inbreeding of the S1 individuals. Inbreeding (f) values varied from 0.15 to 0.89 within...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Partial inbred lines; Manihot esculenta Crantz; Microsatellites; Self-pollination.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703853
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Is the Murciano-Granadina a single goat breed? A molecular genetics approach Arq. Bras. Med. Vet. Zootec.
Martínez,A.M.; Vega-Pla,J.L.; León,J.M.; Camacho,M.E.; Delgado,J.V.; Ribeiro,M.N..
The population structure of the Murciano-Granadina breed was determined using 25 microsatellites from 266 goats of seven populations. The results of the genetic differentiation analysis showed that it is possible to differentiate the Murciana and Granadina populations even though a low F ST value (0.0432) had been obtained. Individuals could be assigned to their populations with a success rate of more than 80%. Bayesian-based clustering analysis of allele frequencies and multivariate analysis revealed that Murciana and Granadina populations were grouped in different clusters since K=3. The results demonstrate that Murciana and Granadina are still two different genetic groups included into Murciano-Granadina denomination. There is the opportunity to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dairy goat; Microsatellites; Genetic diversity; Conservation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000500023
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Discrepancy variation of dinucleotide microsatellite repeats in eukaryotic genomes Biol. Res.
GAO,HUAN; CAI,SHENGLI; YAN,BINLUN; CHEN,BAIYAO; YU,FEI.
To address whether there are differences of variation among repeat motif types and among taxonomic groups, we present here an analysis of variation and correlation of dinucleotide microsatellite repeats in eukaryotic genomes. Ten taxonomic groups were compared, those being primates, mammalia (excluding primates and rodentia), rodentia, birds, fish, amphibians and reptiles, insects, molluscs, plants and fungi, respectively. The data used in the analysis is from the literature published in the Journal of Molecular Ecology Notes. Analysis of variation reveals that there are no significant differences between AC and AG repeat motif types. Moreover, the number of alleles correlates positively with the copy number in both AG and AC repeats. Similar conclusions...
Tipo: Journal article Palavras-chave: Microsatellites; Eukaryotic genomes; Variation of SSR; Copy number; Meta-analysis.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300011
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A comparative genetic diversity assessment of industrial and household Brazilian cassava varieties using SSR markers Bragantia
Siqueira,Marcos Vinicius Bohrer Monteiro; Borges,Aline; Valle,Teresa Losada; Veasey,Elizabeth Ann.
This study was carried out in order to assess the genetic diversity of 20 cassava (Manihot esculenta Crantz) genotypes with high phenotypic performance using microsatellite markers. Two groups were considered for this study: eleven varieties for industrial uses and nine for household consumption. By using nine microsatellite primers, high polymorphism was identified in all the loci analyzed, with values reaching 100%. On average, 3.4 alleles per locus were found, with 0.371 the value estimated for the observed heterozygosity and 0.555 for gene diversity for the entire set of varieties. The genetic variability found in both varieties, cultivated on a large-scale in the South Center region of Brazil, is wide enough to allow the choice of divergent parental...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Manihot esculenta; Genetic diversity; Germplasm; Microsatellites.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052011000400003
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Use of microsatellites for evaluation of genetic diversity in cherry tomato Bragantia
Aguirre,Nelson Ceballos; López,Walter; Orozco-Cárdenas,Martha; Coronado,Yacenia Morillo; Vallejo-Cabrera,Franco.
ABSTRACT Much of the diversity of tomato is found in wild forms, the most important being the Solanum lycopersicum L. var. cerasiforme and S. pimpinellifolium. The objective of this research was to assess the genetic diversity of 30 introductions of cherry tomato with 36 microsatellite molecular markers. The study was conducted at the Plant Transformation Research Center (PTRC) of the University of California. A dendrogram was built using the Dice-Nei and Li similarity index and the UPGMA clustering method, where introductions were differentiated without preserving a distribution pattern obeying the geographical area of origin. A coefficient of genetic differentiation was found (Fst = 0.3474), showing a high genetic differentiation of the introductions;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant genetic resources; Microsatellites; Real-time PCR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200220
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Genetic variability among the wild boars (Sus scrofa scrofa), crossbred animals and pigs using microsatellite markers (STRs) BABT
Silva,Paula Viana Correa da; Lui,Jeffrey Frederico; Band,Guilherme de Oliveira; Regitano,Luciana Correia de Almeida; Grossi,Selma de Fátima; Sollero,Bruna Pena; Nunes,Cleujosí da Silva.
The aim of this work was to study the genetic variability among the wild boars, crossbred animals and pigs using microsatellite markers. Five genetic groups were studied. The fragments of three microsatellites developed for Sus scrofa domestica - IGF1, ACTG2 and TNFB - were amplified through PCR technique to evaluate the expected intra populacion variability (He) and observed (Ho) heterozygosity, and endogamy coefficient (F IS ) within each population and inter population variability F IS , testing relationship among five genetic groups to establish the genetic distance among them. The high level of observed heterozygosity values varied between 0.537 and 0.7871. Generally, F IS was low, suggesting that the endogamy did not exist between the tested animals.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular genetics; Wild boars; Microsatellites; Fragments; Amplification.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200011
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Genetic Variability of Brycon hilarii in a Repopulation Program BABT
Bignardi,Annaiza Braga; Povh,Jayme Aparecido; Alves,Mariana Srucki; Goes,Elenice Souza dos Reis; Filho,Ruy Alberto Caetano Corrêa; Castro,Reinaldo José; Barrero,Nelson Mauricio Lopera.
ABSTRACT Brycon hilarii, popularly called piraputanga in Brazil, is a species distributed throughout the whole basin of the river Paraguay. In recent years, the species has been on a repopulation program due to its remarkable decline as a wild species in the region. Assessment of the genetic diversity of broodstock and fingerling stocks in repopulation programs is basic to avoid genetic impacts on wild populations. The genetic variability of the wild population and of the broodstock and fingerling stocks of B. hilarii in a repopulation program in the river Itiquira MT Brazil will be determined. Seven microsatellite loci produced 52 polymorphic alleles and heterozygosity revealed rates between 0.5794 and 0.7204. FIS did not register any endogamy in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation genetics; Microsatellites; Piraputanga.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100424
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Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by RAPD and SSR markers BABT
Souza,Silvia Graciele Hülse de; Carpentieri-Pípolo,Valéria; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Ruas,Paulo Maurício; Gerage,Antônio Carlos.
The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Corn; Distance genetic; Microsatellites; Molecular markers; Polymorphism; RAPD.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100022
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Evaluation of nine microsatellite loci and misidentification paternity frequency in a population of Gyr breed bovines Braz. J. Vet. Res. Anim. Sci.
Curi,Rogério Abdallah; Lopes,Catalina Romero.
Paternity misidentification is harmful due to the reduction in annual genetic earnings of the population and because it endangers an efficient genetic improvement program. The objectives of the present study was to evaluate nine microsatellites in Paternity Testing and to investigate misidentification paternity frequency in families of Gyr breed bovines population. In the present experiment blood samples from forty Gir breed families ( bull / cow / calf ), registered pure breed in the Zebu Breeders Brazilian Association (ABCZ) were used. The most part of the microsatellites used in this work were recommended by the International Society of Animal Genetics (ISAG). The genomic DNA extraction was performed from whole blood samples. The microsatellites...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA; Microsatellites; Paternity; Bovine; Gyr.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000300004
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Implementation of a Molecular System for Traceability of Beef Based on Microsatellite Markers Chilean J. Agric. Res.
Felmer D,Ricardo; Sagredo D,Boris; Chávez R,Renato; Iraira H,Sergio; Folch M,Carolina; Parra G,Lorena; Catrileo S,Adrián; Ortiz L,Manuel.
Animal products traceability has acquired considerable importance as a security measure in EEC member countries since the food crisis of the mid-nineties. This has led to reinforcing the capacity to manage cattle product quality, with traceability emerging as the main tool to prevent risks to product security and quality as demanded by consumers in developed countries. The practical application of a traceability system for beef, based on molecular markers requires the election of a panel of microsatellites, as well as the optimization of methods of sampling and DNA analysis. In this work, a traceability system for beef based on a panel of 10 microsatellites markers was implemented. Different biological samples were evaluated, such as hair, blood, tissue...
Tipo: Journal article Palavras-chave: Traceability; Microsatellites; Individual identification; Beef.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000400004
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Quantitative trait loci associated with resistance to gray leaf spot and grain yield in corn Ciência e Agrotecnologia
Veiga,Adriano Delly; Pinho,Renzo Garcia Von; Resende,Luciane Vilela; Pinho,Édila Vilela de Resende Von; Balestre,Márcio; Pereira,Laís Andrade.
The main objectives of hybrid development programs include incorporating genetic resistance to diseases and increasing grain yield. Identification of Quantitative Trait Loci (QTL) through the statistical analysis of molecular markers allows efficient selection of resistant and productive hybrids. The objective of this research was to identify QTL associated with resistance to gray leaf spot and for grain yield in the germplasm of tropical corn. We used two strains with different degrees of reaction to the disease; the genotypes are owned by GENESEEDS Ltda, their F1 hybrid and the F2 population. The plants were evaluated for gray leaf spot resistance, for grain yield and were genotyped with 94 microsatellite markers. Association of the markers with the QTL...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maximum likelihood; Microsatellites; Regression.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000100004
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