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	Provedor de dados ArchiMer (2) BJM (1) Autor Leroy, Celine (2) Chai,L.C. (1) Cheah,Y.K. (1) Combes, D (1) Compere, Chantal (1) Delbarre Ladrat, Christine (1) Ghillebaert, F (1) Lee,H.Y. (1) Mustafa,S. (1) Nishibuchi,M. (1) Pui,C.F. (1) Radu,S. (1) Rochet, Marie-joelle (1) Mais... Palavra-chave Microtiter plate (3) Adhesion (2) Antifouling (2) DAPI (2) Marine biofilm (2) Screening (2) Adhésion (1) Antisalissure (1) Biofilm (1) Biofilm marin (1) Criblage (1) EPS (1) Fouling (1) Hydrolases (1) Listeria monocytogenes (1) Microplaque (1) Pseudoalteromonas (1) Pseudoalteromonas sp. (1) Salissure marine (1) Sodium chloride (1) Mais... Tipo do documento Text (2) Info:eu-repo/semantics/article (1) Ano 2013 (1) 2007 (1) 2006 (1) País France (2) Brazil (1) Idioma Inglês (2) Francês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride BJM Lee,H.Y.; Chai,L.C.; Pui,C.F.; Mustafa,S.; Cheah,Y.K.; Nishibuchi,M.; Radu,S.. Biofilm formation can lead to various consequences in the food processing line such as contamination and equipment breakdowns. Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and its biofilm formation ability tested under various concentrations of sodium chloride and temperatures. Cultures of L. monocytogenes ATCC 19112 were placed in 96-well microtitre plate containing concentration of sodium chloride from 1-10% (w/v) and incubated at different temperature of 4 ºC, 30 ºC and 45 ºC for up to 60 h. Absorbance reading of crystal violet staining showed the density of biofilm formed in the 96-well microtitre plates was significantly higher when incubated in 4 ºC. The formation of biofilm... Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Biofilm; Sodium chloride; Temperature; Microtiter plate. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100007 Lutte contre les salissures marines : approche par procédés enzymatiques. ArchiMer Leroy, Celine. Fouling on marine underwater surfaces causes critical and economic problems such as important material biodamages and industrial performances reduction. We chose to test antifouling potential of enzymatic commercial preparations like hydrolases (proteases, glycosidases and lipases) in order to inhibit the first fouling adhesion step: bacterial biofilm formation. An evaluation test of antifouling properties onto marine bacterial adhesion was designed using a mono-incubation of Pseudoalteromonas sp. D41 in microtiter plate and in sterile natural sea water. This test was adapted to screen agents for bacterial adhesion removal or inhibition activities and allowed to test enzymatic preparations toxicity on non adhered bacteria. Inhibition rates according to... Tipo: Text Palavras-chave: Fouling; Adhesion; Antifouling; Hydrolases; EPS; DAPI; Microtiter plate; Screening; Pseudoalteromonas sp.; Marine biofilm; Salissure marine; Adhésion; Antisalissure; Hydrolases; EPS; DAPI; Microplaque; Criblage; Pseudoalteromonas sp.; Biofilm marin. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-1099.pdf A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents ArchiMer Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D. Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test. Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this... Tipo: Text Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf Registros recuperados: 3 Primeira ... 1 ... Última

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