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| Registros recuperados: 208 | |
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| García-Nogales,Paula; Serrano,Alicia; Secchi,Sonia; Gutiérrez,Serafín; Arís,Anna. |
| Nucleic-acid based methods for bacterial identification are extremely useful in diagnostic applications due to their specificity and sensitivity. However, they require an optimal purification of the target molecules. As part of the development of a new diagnostic method for the detection of bacterial RNA in cow milk, we have compared four commercially available RNA extraction kits for the isolation of bacterial RNA from spiked UHT milk samples. The kits were compared in terms of extraction efficiency and RNA purity using two bacterial species, the Gram negative Escherichia coli and the Gram positive Staphylococcus aureus. Two kits are based in silica-matrix extraction, and the other two in the guanidinium thiocyanate-phenol-chloroform extraction. In our... |
| Tipo: Journal article |
Palavras-chave: Bacteria; Milk; Purification; RNA. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500019 |
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| Gigli,Isabel; Maizon,Daniel Omar. |
| MicroRNAs (miRNAs) have been identified in cells as well as in exosomes in biological fluids such as milk. In mammary gland, most of the miRNAs studied have functions related to immunity and show alterations in their pattern of expression during lactation. In mastitis, the inflammatory response caused by Streptococcus uberis alters the expression of miRNAs that may regulate the innate immune system. These small RNAs are stable at room temperature and are resistant to repeated freeze/thaw cycles, acidic conditions and degradation by RNAse, making them resistant to industrial procedures. These properties mean that miRNAs could have multiple applications in veterinary medicine and biotechnology. Indeed, lactoglobulin-free milk has been produced in transgenic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactation; Mastitis; Milk; MiRNA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400001 |
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| MARQUES, J. R. F.; NASCIMENTO, C. N. B. do; CARVALHO, L. O. D. de M.; LOURENÇO JUNIOR, J. de B.; COSTA, N. A. da; BATISTA, H. A. M.; RIBEIRO, M. J. P. da S. de S.; BARBOSA, C.; RAMOS, A. de A.; PIMENTEL, E. S.. |
| bitstream/item/41643/1/Boletim-Pesquisa-121-CPATU.pdf |
| Tipo: Séries anteriores (INFOTECA-E) |
Palavras-chave: Percentagem; Fonte de variação; Bubalino; Característica; Estatística descritiva; Melhoramento genético; Fat percentage; Genetic breeding; Management; Sources of variation; Buffalo; Characteristic.; Animal; Gordura; Leite; Lactação; Manejo.; Lactation; Milk; Statistics.. |
| Ano: 1991 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/383556 |
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| Registros recuperados: 208 | |
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